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The map label for this gene is braC [H]

Identifier: 220904746

GI number: 220904746

Start: 1768920

End: 1770050

Strand: Direct

Name: braC [H]

Synonym: Ddes_1478

Alternate gene names: 220904746

Gene position: 1768920-1770050 (Clockwise)

Preceding gene: 220904745

Following gene: 220904747

Centisome position: 61.56

GC content: 58.8

Gene sequence:

>1131_bases
ATGAAAAAAGGTATAACCTTGCTTGCGGCAATGGCACTCTGCGCGGTTCTGGCCGCCCCCGCTCAGGCGGAAGACACCAT
CAAAATCGGTGTCGCCGGTGCTCACTCCGGCGATCTGGCCTCCTACGGCGTACCCAGCCTAAACGCTGCCAAGGTGGTCA
TTGCCGATGTCAACGCCAAGGGCGGCATACTCGGCAAGCAGGTCGAACTGGTAGCCCAGGATGACCAGTGCAAACCCGAA
CTGGCTACCAACGCGGCCACCAAGCTCATCTCCGACAAGGTGGCTGTGGTGATGGGGCATATCTGCTCCGGCGCCACCAA
GGCCACACTGCCCCTGTATACCGATGCCAAAATCGTGAGCATGTCGCCCTCTGCCACCACTCCCGGTCTCACCGAAGACG
GCAACAACCCGTACTTCTTCCGCACCATCGCCAATGACAAGGCCCAGGCCAGGTTGACCAGCGACTTCATGCTCAACAAC
CTCAAGGTCAAAAAGGTTGCCTATCTGCATGATAACGGCGACTACGGCAAAGGCTTTGTGGACAATAACCGCGAGCACCT
CGAAAAGGCTGGCGTCAAAACAGTTCTGTTTGAAGCCATTACCCCCGATGCCGTGGACTTTTCCGCTGTTGTGCGCAAAC
TGAGACGCGAACAGCCCGACATCGTGGTTTTCGGCGGGTATCAGCCCACGGCCTCCAAGCTGTTGCAGCAGATGCGTCGC
GACCGCGTGACCACCGCCCTGCTCGGCCCTGACGGGCTTATGGACGAGGCCTTCATCAAGATGGCCGGCAAGGCCAGCGA
AGGCGTGTACTCTTCCTACCCCAAAGACACCAGCAATCTGCCCGCTTACAGGGCCGCCCGTGAAGGCCACGTAAAGATGT
TCGGCAAGGATCCCGGCTCGTTCTACTACAACGGCTATGCCGCCACCCAGGCTCTGGTCAATGCCATTGAAAAGGCCGGC
AGCACCGACACAGCCAAGGTTGTGGATGCCCTGCGCTCCGCACCTGTGGATACCCCGCTGGGCACCCTGACTTTCAGCAA
AACCGGCGATGCCGCCGGCATGGCTCTTTCCATCTATCAGGTCAAAGACGGAAAATTTGTGGAACTCGACCACAGCATCG
TCCTTGAGTAG

Upstream 100 bases:

>100_bases
CAACCCGCATTGACAGGCCGCCCGCAATAAGGCAATAGAGAAGCCTTCATCCATTTTGAAGCGCAAAATACGCGCGACAA
TTTTCTCGTAGGAGGGGCTG

Downstream 100 bases:

>100_bases
TTGATCAAGGGGGCGCGACCATGGTCGCGCCCCCGTTTTCGCACACACTTTCGGCTTCGGCCGACGGCAATAACGGAACT
GACGCCGGGAGCATGGCATT

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKKGITLLAAMALCAVLAAPAQAEDTIKIGVAGAHSGDLASYGVPSLNAAKVVIADVNAKGGILGKQVELVAQDDQCKPE
LATNAATKLISDKVAVVMGHICSGATKATLPLYTDAKIVSMSPSATTPGLTEDGNNPYFFRTIANDKAQARLTSDFMLNN
LKVKKVAYLHDNGDYGKGFVDNNREHLEKAGVKTVLFEAITPDAVDFSAVVRKLRREQPDIVVFGGYQPTASKLLQQMRR
DRVTTALLGPDGLMDEAFIKMAGKASEGVYSSYPKDTSNLPAYRAAREGHVKMFGKDPGSFYYNGYAATQALVNAIEKAG
STDTAKVVDALRSAPVDTPLGTLTFSKTGDAAGMALSIYQVKDGKFVELDHSIVLE

Sequences:

>Translated_376_residues
MKKGITLLAAMALCAVLAAPAQAEDTIKIGVAGAHSGDLASYGVPSLNAAKVVIADVNAKGGILGKQVELVAQDDQCKPE
LATNAATKLISDKVAVVMGHICSGATKATLPLYTDAKIVSMSPSATTPGLTEDGNNPYFFRTIANDKAQARLTSDFMLNN
LKVKKVAYLHDNGDYGKGFVDNNREHLEKAGVKTVLFEAITPDAVDFSAVVRKLRREQPDIVVFGGYQPTASKLLQQMRR
DRVTTALLGPDGLMDEAFIKMAGKASEGVYSSYPKDTSNLPAYRAAREGHVKMFGKDPGSFYYNGYAATQALVNAIEKAG
STDTAKVVDALRSAPVDTPLGTLTFSKTGDAAGMALSIYQVKDGKFVELDHSIVLE
>Mature_376_residues
MKKGITLLAAMALCAVLAAPAQAEDTIKIGVAGAHSGDLASYGVPSLNAAKVVIADVNAKGGILGKQVELVAQDDQCKPE
LATNAATKLISDKVAVVMGHICSGATKATLPLYTDAKIVSMSPSATTPGLTEDGNNPYFFRTIANDKAQARLTSDFMLNN
LKVKKVAYLHDNGDYGKGFVDNNREHLEKAGVKTVLFEAITPDAVDFSAVVRKLRREQPDIVVFGGYQPTASKLLQQMRR
DRVTTALLGPDGLMDEAFIKMAGKASEGVYSSYPKDTSNLPAYRAAREGHVKMFGKDPGSFYYNGYAATQALVNAIEKAG
STDTAKVVDALRSAPVDTPLGTLTFSKTGDAAGMALSIYQVKDGKFVELDHSIVLE

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=361, Percent_Identity=33.5180055401662, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI48994941, Length=360, Percent_Identity=34.7222222222222, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39899; Mature: 39899

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGITLLAAMALCAVLAAPAQAEDTIKIGVAGAHSGDLASYGVPSLNAAKVVIADVNAK
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCCEEEEEECCCC
GGILGKQVELVAQDDQCKPELATNAATKLISDKVAVVMGHICSGATKATLPLYTDAKIVS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
MSPSATTPGLTEDGNNPYFFRTIANDKAQARLTSDFMLNNLKVKKVAYLHDNGDYGKGFV
ECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCC
DNNREHLEKAGVKTVLFEAITPDAVDFSAVVRKLRREQPDIVVFGGYQPTASKLLQQMRR
CCCHHHHHHCCHHHEEHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
DRVTTALLGPDGLMDEAFIKMAGKASEGVYSSYPKDTSNLPAYRAAREGHVKMFGKDPGS
HHHEEEEECCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHCCCCCEEEEECCCCC
FYYNGYAATQALVNAIEKAGSTDTAKVVDALRSAPVDTPLGTLTFSKTGDAAGMALSIYQ
EEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEE
VKDGKFVELDHSIVLE
ECCCCEEEECCEEECC
>Mature Secondary Structure
MKKGITLLAAMALCAVLAAPAQAEDTIKIGVAGAHSGDLASYGVPSLNAAKVVIADVNAK
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCCEEEEEECCCC
GGILGKQVELVAQDDQCKPELATNAATKLISDKVAVVMGHICSGATKATLPLYTDAKIVS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
MSPSATTPGLTEDGNNPYFFRTIANDKAQARLTSDFMLNNLKVKKVAYLHDNGDYGKGFV
ECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCC
DNNREHLEKAGVKTVLFEAITPDAVDFSAVVRKLRREQPDIVVFGGYQPTASKLLQQMRR
CCCHHHHHHCCHHHEEHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
DRVTTALLGPDGLMDEAFIKMAGKASEGVYSSYPKDTSNLPAYRAAREGHVKMFGKDPGS
HHHEEEEECCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHCCCCCEEEEECCCCC
FYYNGYAATQALVNAIEKAGSTDTAKVVDALRSAPVDTPLGTLTFSKTGDAAGMALSIYQ
EEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEE
VKDGKFVELDHSIVLE
ECCCCEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]