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The map label for this gene is mdeA [H]

Identifier: 220904736

GI number: 220904736

Start: 1757941

End: 1759233

Strand: Direct

Name: mdeA [H]

Synonym: Ddes_1468

Alternate gene names: 220904736

Gene position: 1757941-1759233 (Clockwise)

Preceding gene: 220904735

Following gene: 220904737

Centisome position: 61.18

GC content: 59.24

Gene sequence:

>1293_bases
ATGACTGACAATAAAAACAGCCTGCGTTTTGAAACGCTGCAGGTACATGCAGGCCAGGAAAAACCGGATTCGGCCAGCGG
CGCACGGGCAGTACCCATCTATCAGACCACATCATATGTGTTCAACGACTGTGCCCATGCCGAGGCGCGCTTCAACCTGA
CCGACCCCGGCAACATATACAGCCGGCTGACCAATCCCACGCAAGACGCCCTGGAGCAGCGCGTGGCGGCCCTTGAAGGC
GGTGTGGCCGCCCTGGCAACGGCCAGCGGCGCGGCGGCTGTGAGCTATGCCCTGCAAAACCTGGCCCGGGCCGGAGACCA
TATTGTGGCGGCAAAAACCCTCTATGGCGGAACCTACAACCTGCTTGCCCATACCTTCAAGGACTCGGGCATTGAAACCA
CCTTTGTGGACCCGGGGCAGACAGACTTTTTCGAGCCAGCCATAACCCCCCGCACACGTGCGATCTTTGTGGAGAGCATG
GGCAACCCCCACAGCAATATCGTGGATATGAAGGCTCTGGCAAACCTTGCCCATCACCACTCCATCCCGCTGGTAGTGGA
CAATACCTTTGCCACGCCATGGCTCATGCGTCCCATTGAGCACGGCGCGGATATCGTTGTTCACTCGGCGACCAAGTTTA
TGGGCGGTCATGGCGCAGCTCTGGGCGGAATTATTGTGGATGGCGGCCACTTTGACTGGGCCTCTGCCGGAAAGTTTTCC
CACCTGAGTGAGCCGGACCCGAGCTATCACGGGCTGAGCTTCACAAAGGCCGTCGGGGCGGCGGCCTATATAGTGCGGGC
GCGGGCCATTCTGCTGCGCGATCTGGGCGCAGCTATGGCCCCCCTGCACGCCTTTCTCATCCTTCAGGGGCTTGAAACCC
TGTCTCTGCGTGTGGAACGCCACGTGCAAAACGCTCTGGCCGTGGTGCGCCATCTGCATAAGCATCCCAAGGTCGAAGCC
GTCAATCATCCGAGCCTGCCCCAAAGCCCAAGTCATGCTCTGTACAAACGCTACTTTCCGCACGGCGGAGGCAGCATCTT
TACCATAGAAATCAAAGGCGGCGCAGCAGAAGCCAGGGCCTTTATCGACAGGCTGCGCATATTCTCGCTGCTGGCCAACG
TGGCGGACGCAAAGTCGCTGGTCATACATCCCGCGTCCACCACGCATTCACAAATGACGGAACAGGAACTGGCAAGCACA
GGCATACGGCCCAATACCGTGCGCCTGTCCATCGGCATCGAGCATATTGACGATATTCTTGCCGATCTGGACCAGGCCCT
GGCTGCCCTGTAA

Upstream 100 bases:

>100_bases
CCACTGTTTCCGCCAACCTATTTCATACCATATTGATAAGAAATAGCTGGGACCTGTTGAATACCGAAAAAAACAATGCC
GGCCGCCAAGGGGACAGATC

Downstream 100 bases:

>100_bases
TCCCCATCGTCCGGCGGTGAAGGACGCAGTGCAGAAGACCTTTGTCTGCCCCGCGTGACGCCGCCGGGCATGGCGTTTCT
GCCCTGCCATATGTACACTG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTDNKNSLRFETLQVHAGQEKPDSASGARAVPIYQTTSYVFNDCAHAEARFNLTDPGNIYSRLTNPTQDALEQRVAALEG
GVAALATASGAAAVSYALQNLARAGDHIVAAKTLYGGTYNLLAHTFKDSGIETTFVDPGQTDFFEPAITPRTRAIFVESM
GNPHSNIVDMKALANLAHHHSIPLVVDNTFATPWLMRPIEHGADIVVHSATKFMGGHGAALGGIIVDGGHFDWASAGKFS
HLSEPDPSYHGLSFTKAVGAAAYIVRARAILLRDLGAAMAPLHAFLILQGLETLSLRVERHVQNALAVVRHLHKHPKVEA
VNHPSLPQSPSHALYKRYFPHGGGSIFTIEIKGGAAEARAFIDRLRIFSLLANVADAKSLVIHPASTTHSQMTEQELAST
GIRPNTVRLSIGIEHIDDILADLDQALAAL

Sequences:

>Translated_430_residues
MTDNKNSLRFETLQVHAGQEKPDSASGARAVPIYQTTSYVFNDCAHAEARFNLTDPGNIYSRLTNPTQDALEQRVAALEG
GVAALATASGAAAVSYALQNLARAGDHIVAAKTLYGGTYNLLAHTFKDSGIETTFVDPGQTDFFEPAITPRTRAIFVESM
GNPHSNIVDMKALANLAHHHSIPLVVDNTFATPWLMRPIEHGADIVVHSATKFMGGHGAALGGIIVDGGHFDWASAGKFS
HLSEPDPSYHGLSFTKAVGAAAYIVRARAILLRDLGAAMAPLHAFLILQGLETLSLRVERHVQNALAVVRHLHKHPKVEA
VNHPSLPQSPSHALYKRYFPHGGGSIFTIEIKGGAAEARAFIDRLRIFSLLANVADAKSLVIHPASTTHSQMTEQELAST
GIRPNTVRLSIGIEHIDDILADLDQALAAL
>Mature_429_residues
TDNKNSLRFETLQVHAGQEKPDSASGARAVPIYQTTSYVFNDCAHAEARFNLTDPGNIYSRLTNPTQDALEQRVAALEGG
VAALATASGAAAVSYALQNLARAGDHIVAAKTLYGGTYNLLAHTFKDSGIETTFVDPGQTDFFEPAITPRTRAIFVESMG
NPHSNIVDMKALANLAHHHSIPLVVDNTFATPWLMRPIEHGADIVVHSATKFMGGHGAALGGIIVDGGHFDWASAGKFSH
LSEPDPSYHGLSFTKAVGAAAYIVRARAILLRDLGAAMAPLHAFLILQGLETLSLRVERHVQNALAVVRHLHKHPKVEAV
NHPSLPQSPSHALYKRYFPHGGGSIFTIEIKGGAAEARAFIDRLRIFSLLANVADAKSLVIHPASTTHSQMTEQELASTG
IRPNTVRLSIGIEHIDDILADLDQALAAL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=34.6793349168646, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=31.8289786223278, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=30.952380952381, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1790375, Length=418, Percent_Identity=30.1435406698565, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=26.8691588785047, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=34.2789598108747, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI71980708, Length=433, Percent_Identity=29.7921478060046, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=34.1232227488152, Blast_Score=201, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=47.4654377880184, Blast_Score=355, Evalue=7e-99,
Organism=Saccharomyces cerevisiae, GI6319307, Length=429, Percent_Identity=31.2354312354312, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=377, Percent_Identity=29.7082228116711, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=24.2696629213483, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=25.4079254079254, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=34.9881796690307, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46128; Mature: 45997

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNKNSLRFETLQVHAGQEKPDSASGARAVPIYQTTSYVFNDCAHAEARFNLTDPGNIY
CCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCEEECCCCCHHHH
SRLTNPTQDALEQRVAALEGGVAALATASGAAAVSYALQNLARAGDHIVAAKTLYGGTYN
HHCCCCHHHHHHHHHHHHHCCHHEEHHCCCHHHHHHHHHHHHHCCCCEEEEHHHCCCHHH
LLAHTFKDSGIETTFVDPGQTDFFEPAITPRTRAIFVESMGNPHSNIVDMKALANLAHHH
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
SIPLVVDNTFATPWLMRPIEHGADIVVHSATKFMGGHGAALGGIIVDGGHFDWASAGKFS
CCCEEEECCCCCHHHHHHHHCCCCEEEECHHHHCCCCCCEECCEEEECCCCCCCCCCCCC
HLSEPDPSYHGLSFTKAVGAAAYIVRARAILLRDLGAAMAPLHAFLILQGLETLSLRVER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVQNALAVVRHLHKHPKVEAVNHPSLPQSPSHALYKRYFPHGGGSIFTIEIKGGAAEARA
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHH
FIDRLRIFSLLANVADAKSLVIHPASTTHSQMTEQELASTGIRPNTVRLSIGIEHIDDIL
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
ADLDQALAAL
HHHHHHHHCC
>Mature Secondary Structure 
TDNKNSLRFETLQVHAGQEKPDSASGARAVPIYQTTSYVFNDCAHAEARFNLTDPGNIY
CCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCEEECCCCCHHHH
SRLTNPTQDALEQRVAALEGGVAALATASGAAAVSYALQNLARAGDHIVAAKTLYGGTYN
HHCCCCHHHHHHHHHHHHHCCHHEEHHCCCHHHHHHHHHHHHHCCCCEEEEHHHCCCHHH
LLAHTFKDSGIETTFVDPGQTDFFEPAITPRTRAIFVESMGNPHSNIVDMKALANLAHHH
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
SIPLVVDNTFATPWLMRPIEHGADIVVHSATKFMGGHGAALGGIIVDGGHFDWASAGKFS
CCCEEEECCCCCHHHHHHHHCCCCEEEECHHHHCCCCCCEECCEEEECCCCCCCCCCCCC
HLSEPDPSYHGLSFTKAVGAAAYIVRARAILLRDLGAAMAPLHAFLILQGLETLSLRVER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVQNALAVVRHLHKHPKVEAVNHPSLPQSPSHALYKRYFPHGGGSIFTIEIKGGAAEARA
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHH
FIDRLRIFSLLANVADAKSLVIHPASTTHSQMTEQELASTGIRPNTVRLSIGIEHIDDIL
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
ADLDQALAAL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]