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The map label for this gene is glpK

Identifier: 220904615

GI number: 220904615

Start: 1617882

End: 1619378

Strand: Reverse

Name: glpK

Synonym: Ddes_1347

Alternate gene names: 220904615

Gene position: 1619378-1617882 (Counterclockwise)

Preceding gene: 220904616

Following gene: 220904614

Centisome position: 56.36

GC content: 56.38

Gene sequence:

>1497_bases
ATGGCCCCACGCTACATACTGGCCCTTGACCAGGGTACAACCAGTTCACGCGCGATCCTTTTTGATCGTGATGCCAACAT
AGTGCAGGTTGCGCAAAAAGAGTTTACCCAGATATATCCTGAGCCAGGGTGGGTCGAGCATTCGCCCAATGAAATTTTTG
ATTCGCAGGCGCAGGTTCTGCATGAATGCCTGCGGCTCGCACGAGTGAGCGGCAATGAAATTGCGGCCGTAGGTATAGCT
AATCAACGGGAAACTACCATCGTGTGGGAGAAGTCTACCGGGGCGCCTGTTTATAACGCCATTGTCTGGCAGGACCGGCG
TACAGCGGCCTTTTGTGATCAGTTGCGGGTTGACGGCAAAGCGCACATTATTCAGGAAAAGACAGGGCTTGTGCTGGATG
CATATTTTTCCGGCAGCAAGGTGCGCTGGATACTGGATAATGTACCGGGAGCCAGGGCAAAGGCCGATGCCGGAGAATTA
CTGTTCGGCACGGTGGATTCCTGGATTGTGTGGAACCTGAGCAAGCGGGGAACCCACGTCACGGACCCGTCCAATGCCAG
CCGGACGATGATGTTCAATATCCACACGGGGCAGTGGGACAGCGAACTGCTTGAGATTGTGGGGGTGCCGCCCTCCATGC
TGCCCGAGGTCGTCCCTTCATCCAGTCATCTGGCCGAGGTACATCCTGAATTTTTTGGCCGGGCGGTGCCTGTCGCTGGT
ATTGCCGGTGATCAGCAGGCCGCCACCTATGGCAACGCCTGCATGAGCCTGGGGATGGCAAAAAATACCTATGGCACGGG
CTGTTTCCTGTTGCTTAACACCGGGCATAGTCCGCGCGCCAGCTTGAACAATATGCTCACCACTGTGGCGTGGCAGACTG
CGGGGGGATATTCCTACGCGCTCGAAGGCAGTGTTTTTGTGGGCGGTGCGGTGGTGCAGTGGTTGCGTGACGGGTTGGGC
ATCATCAAAGATTCTTCAGAAATGGAAAGGCTGGCCGGTACTGTGCCGGATAATGGCGGGGTTTATATGGTTCCTGCCTT
TGTGGGCCTTGGCGCACCCTATTGGGATCAGTACGCCCGGGGGACCATCGTGGGCCTGACCAGGGGAACCGGCAGAGGAC
ATATCGCCCGAGCTGCAACTGAAGCCATTGCGTTGCAGACACTGGATATTATTGATGCCATGCAAAAAGACACGGGTATT
GCCCTTACGGCGCTTCGGGTGGACGGCGGCGCCAGCCGCAACAACATGCTCATGCAGTTTCAGGCGGACCTCACAGGGGT
TCTGGTTGAGCGGCCCACAGTAACAGAAACGACTGCCCTCGGGGTTGCCTATCTTGCCGGACTTGCCACAGGTTTTTGGG
AAAATGAAGAAGAGCTGGCAAGTTTGTGGAAACTTGATCGCTGTTTTTCTCCCTCAATGCCCGGAGACAGGAGAGACGAG
CTGCTGTATCAGTGGCATCGCGCGGTGCAAAGAGCTGCGCACTGGCTTGAGGAGTAG

Upstream 100 bases:

>100_bases
TGTTGTCCATGTGACCTGCTTCTGTTCAAACAGGACGGAGATGTGTACCATCGCTCAAGAGCGAAACGGCAGAGCCTCAC
GGCAAGAGCGGAGGAATACT

Downstream 100 bases:

>100_bases
GGCAGAACAGAAAATTTGCAGGACGGCTGCAAGCAGCATGATGAACCTGTGGCTTTTGTCTGTTTTTGTTCAGGGCAGCA
GAGGTTCTTGACTTATCCCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAPRYILALDQGTTSSRAILFDRDANIVQVAQKEFTQIYPEPGWVEHSPNEIFDSQAQVLHECLRLARVSGNEIAAVGIA
NQRETTIVWEKSTGAPVYNAIVWQDRRTAAFCDQLRVDGKAHIIQEKTGLVLDAYFSGSKVRWILDNVPGARAKADAGEL
LFGTVDSWIVWNLSKRGTHVTDPSNASRTMMFNIHTGQWDSELLEIVGVPPSMLPEVVPSSSHLAEVHPEFFGRAVPVAG
IAGDQQAATYGNACMSLGMAKNTYGTGCFLLLNTGHSPRASLNNMLTTVAWQTAGGYSYALEGSVFVGGAVVQWLRDGLG
IIKDSSEMERLAGTVPDNGGVYMVPAFVGLGAPYWDQYARGTIVGLTRGTGRGHIARAATEAIALQTLDIIDAMQKDTGI
ALTALRVDGGASRNNMLMQFQADLTGVLVERPTVTETTALGVAYLAGLATGFWENEEELASLWKLDRCFSPSMPGDRRDE
LLYQWHRAVQRAAHWLEE

Sequences:

>Translated_498_residues
MAPRYILALDQGTTSSRAILFDRDANIVQVAQKEFTQIYPEPGWVEHSPNEIFDSQAQVLHECLRLARVSGNEIAAVGIA
NQRETTIVWEKSTGAPVYNAIVWQDRRTAAFCDQLRVDGKAHIIQEKTGLVLDAYFSGSKVRWILDNVPGARAKADAGEL
LFGTVDSWIVWNLSKRGTHVTDPSNASRTMMFNIHTGQWDSELLEIVGVPPSMLPEVVPSSSHLAEVHPEFFGRAVPVAG
IAGDQQAATYGNACMSLGMAKNTYGTGCFLLLNTGHSPRASLNNMLTTVAWQTAGGYSYALEGSVFVGGAVVQWLRDGLG
IIKDSSEMERLAGTVPDNGGVYMVPAFVGLGAPYWDQYARGTIVGLTRGTGRGHIARAATEAIALQTLDIIDAMQKDTGI
ALTALRVDGGASRNNMLMQFQADLTGVLVERPTVTETTALGVAYLAGLATGFWENEEELASLWKLDRCFSPSMPGDRRDE
LLYQWHRAVQRAAHWLEE
>Mature_497_residues
APRYILALDQGTTSSRAILFDRDANIVQVAQKEFTQIYPEPGWVEHSPNEIFDSQAQVLHECLRLARVSGNEIAAVGIAN
QRETTIVWEKSTGAPVYNAIVWQDRRTAAFCDQLRVDGKAHIIQEKTGLVLDAYFSGSKVRWILDNVPGARAKADAGELL
FGTVDSWIVWNLSKRGTHVTDPSNASRTMMFNIHTGQWDSELLEIVGVPPSMLPEVVPSSSHLAEVHPEFFGRAVPVAGI
AGDQQAATYGNACMSLGMAKNTYGTGCFLLLNTGHSPRASLNNMLTTVAWQTAGGYSYALEGSVFVGGAVVQWLRDGLGI
IKDSSEMERLAGTVPDNGGVYMVPAFVGLGAPYWDQYARGTIVGLTRGTGRGHIARAATEAIALQTLDIIDAMQKDTGIA
LTALRVDGGASRNNMLMQFQADLTGVLVERPTVTETTALGVAYLAGLATGFWENEEELASLWKLDRCFSPSMPGDRRDEL
LYQWHRAVQRAAHWLEE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=44.8207171314741, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=44.8207171314741, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=44.3786982248521, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=43.0278884462151, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI88196792, Length=515, Percent_Identity=30.873786407767, Blast_Score=295, Evalue=8e-80,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=56.7676767676768, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1790005, Length=518, Percent_Identity=24.1312741312741, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787789, Length=512, Percent_Identity=25.1953125, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1789168, Length=501, Percent_Identity=24.5508982035928, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789987, Length=479, Percent_Identity=21.7118997912317, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=41.8650793650794, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=26.1950286806883, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI71986147, Length=296, Percent_Identity=31.0810810810811, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=561, Percent_Identity=37.7896613190731, Blast_Score=311, Evalue=2e-85,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=41.2109375, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=40.1197604790419, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=40.1197604790419, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=40.1197604790419, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=40.1197604790419, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=40.1197604790419, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24654573, Length=452, Percent_Identity=42.4778761061947, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=30.4511278195489, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24652871, Length=499, Percent_Identity=31.062124248497, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24656367, Length=521, Percent_Identity=30.3262955854127, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI21357867, Length=536, Percent_Identity=29.6641791044776, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24656372, Length=353, Percent_Identity=32.2946175637394, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=29.6551724137931, Blast_Score=131, Evalue=9e-31,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_DESDA (B8J0H2)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479927.1
- ProteinModelPortal:   B8J0H2
- GeneID:   7285041
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1347
- HOGENOM:   HBG511469
- ProtClustDB:   CLSK706192
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54258; Mature: 54127

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRYILALDQGTTSSRAILFDRDANIVQVAQKEFTQIYPEPGWVEHSPNEIFDSQAQVL
CCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
HECLRLARVSGNEIAAVGIANQRETTIVWEKSTGAPVYNAIVWQDRRTAAFCDQLRVDGK
HHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHCCCCC
AHIIQEKTGLVLDAYFSGSKVRWILDNVPGARAKADAGELLFGTVDSWIVWNLSKRGTHV
EEEEECCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCC
TDPSNASRTMMFNIHTGQWDSELLEIVGVPPSMLPEVVPSSSHLAEVHPEFFGRAVPVAG
CCCCCCCEEEEEEEECCCCHHHHHHHHCCCHHHHHHHCCCCCHHHHCCHHHCCCCCCEEE
IAGDQQAATYGNACMSLGMAKNTYGTGCFLLLNTGHSPRASLNNMLTTVAWQTAGGYSYA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEEEECCCCEEEE
LEGSVFVGGAVVQWLRDGLGIIKDSSEMERLAGTVPDNGGVYMVPAFVGLGAPYWDQYAR
ECCCEEEHHHHHHHHHCCCCEECCCHHHHHHHCCCCCCCCEEEEEHHHHCCCCHHHHHHC
GTIVGLTRGTGRGHIARAATEAIALQTLDIIDAMQKDTGIALTALRVDGGASRNNMLMQF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEE
QADLTGVLVERPTVTETTALGVAYLAGLATGFWENEEELASLWKLDRCFSPSMPGDRRDE
ECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LLYQWHRAVQRAAHWLEE
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APRYILALDQGTTSSRAILFDRDANIVQVAQKEFTQIYPEPGWVEHSPNEIFDSQAQVL
CCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
HECLRLARVSGNEIAAVGIANQRETTIVWEKSTGAPVYNAIVWQDRRTAAFCDQLRVDGK
HHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHCCCCC
AHIIQEKTGLVLDAYFSGSKVRWILDNVPGARAKADAGELLFGTVDSWIVWNLSKRGTHV
EEEEECCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCC
TDPSNASRTMMFNIHTGQWDSELLEIVGVPPSMLPEVVPSSSHLAEVHPEFFGRAVPVAG
CCCCCCCEEEEEEEECCCCHHHHHHHHCCCHHHHHHHCCCCCHHHHCCHHHCCCCCCEEE
IAGDQQAATYGNACMSLGMAKNTYGTGCFLLLNTGHSPRASLNNMLTTVAWQTAGGYSYA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEEEECCCCEEEE
LEGSVFVGGAVVQWLRDGLGIIKDSSEMERLAGTVPDNGGVYMVPAFVGLGAPYWDQYAR
ECCCEEEHHHHHHHHHCCCCEECCCHHHHHHHCCCCCCCCEEEEEHHHHCCCCHHHHHHC
GTIVGLTRGTGRGHIARAATEAIALQTLDIIDAMQKDTGIALTALRVDGGASRNNMLMQF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEE
QADLTGVLVERPTVTETTALGVAYLAGLATGFWENEEELASLWKLDRCFSPSMPGDRRDE
ECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LLYQWHRAVQRAAHWLEE
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA