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The map label for this gene is mraW

Identifier: 220904376

GI number: 220904376

Start: 1296229

End: 1297212

Strand: Direct

Name: mraW

Synonym: Ddes_1105

Alternate gene names: 220904376

Gene position: 1296229-1297212 (Clockwise)

Preceding gene: 220904375

Following gene: 220904377

Centisome position: 45.11

GC content: 60.57

Gene sequence:

>984_bases
ATGATACAGACTATGAGCGATACTACGGAACCGACACGGCATGTGTCCGTTCTGCCTGTGGAAACCCTGGCGGCGCTCGA
TCCGCGCGCCGGGGGACGTTATCTGGACGGCACTCTTGGCATGGGCGGACATGCCAGCGCCATTTTGTCGTCTGCACCCG
GCATAGAGCTGTGCGGGCTTGACCGTGATGAAGAGGCGCTTGCCCTTGCCGGGCAGCGTTTGAGCAGTTTCGGCGGGCGA
GCGCACCTGTTTCACTGCCGCTACAGTGATTTTGCCGAAGCCCTCAACGAACTTGGCTGGGACAAGGTGGATGGAGCCTT
GTTGGATATCGGCGTTTCGTCATTGCAACTGGATGAGGCGGAACGTGGCTTCAGTTTTTATGGTGATGGCCCTCTGGACA
TGCGGATGGACCAGAATTCGGCGCAGCCTTCGGCGTGGCACTGGGTCAATCGCGAAAGCTTTGACAAGCTCAAAGACTGC
ATCGCCACCCTGGGTGAAGAGCCGCAAGCCGGGCGCATTGCCCGCGCCATTGTGGACGCCAGGCAGAAAAACACCATCGA
TACGACAGGCCAGCTGGCTGCGCTGGTTGAGAGGGCGTACCCGGCCGCCTGGCGGGCCAAAGCCCGTCGGCATCCAGCCA
CACGGACCTTTCAGGCCTTGCGCATGGCCGTTAACGACGAGCTTGGTGAGCTGCGCCGTTTTCTGGATCAAATCCTGACA
TGGCTGCCCATTGGCGGGCGGCTGGCCGTCATAACGTTCCACTCGCTTGAGGACCGTATGGTCAAGCAGGCCATGCGGCA
CTGGGTCGAAGGTTGCCGTTGTCCGCGCCATATTCCCCGTTGCATATGCGGTCATCAGCCCGAAGTGCGCATTCTGCATA
AAAAACCCGTCCAGGCCGGGCCGGAAGAACTGGCAGCAAATTCCCGGGCGGGCAGCGCCAAACTGCGCGCTGTAGAGAAA
ATTGCCGAGGACTCCGGCTCGTGA

Upstream 100 bases:

>100_bases
TGAAATCTGGGATCAGATCCGCTTTGACGCCCTGGAGCTTGAAGACGTTTCTGCGGAGCTTGCAGCCAGCGGTGTGGCGA
TCAGCCTTTAGGCGCTATTT

Downstream 100 bases:

>100_bases
AGCGCAATCTTTCGGTAAAGCCCACCGGGGGCAGGGGATGGCTGCTTTTTCTGGTGCTTGGTTTGCTGGCCTGCATGGTT
ATGGGTCTTGTGCTCGTGTG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MIQTMSDTTEPTRHVSVLPVETLAALDPRAGGRYLDGTLGMGGHASAILSSAPGIELCGLDRDEEALALAGQRLSSFGGR
AHLFHCRYSDFAEALNELGWDKVDGALLDIGVSSLQLDEAERGFSFYGDGPLDMRMDQNSAQPSAWHWVNRESFDKLKDC
IATLGEEPQAGRIARAIVDARQKNTIDTTGQLAALVERAYPAAWRAKARRHPATRTFQALRMAVNDELGELRRFLDQILT
WLPIGGRLAVITFHSLEDRMVKQAMRHWVEGCRCPRHIPRCICGHQPEVRILHKKPVQAGPEELAANSRAGSAKLRAVEK
IAEDSGS

Sequences:

>Translated_327_residues
MIQTMSDTTEPTRHVSVLPVETLAALDPRAGGRYLDGTLGMGGHASAILSSAPGIELCGLDRDEEALALAGQRLSSFGGR
AHLFHCRYSDFAEALNELGWDKVDGALLDIGVSSLQLDEAERGFSFYGDGPLDMRMDQNSAQPSAWHWVNRESFDKLKDC
IATLGEEPQAGRIARAIVDARQKNTIDTTGQLAALVERAYPAAWRAKARRHPATRTFQALRMAVNDELGELRRFLDQILT
WLPIGGRLAVITFHSLEDRMVKQAMRHWVEGCRCPRHIPRCICGHQPEVRILHKKPVQAGPEELAANSRAGSAKLRAVEK
IAEDSGS
>Mature_327_residues
MIQTMSDTTEPTRHVSVLPVETLAALDPRAGGRYLDGTLGMGGHASAILSSAPGIELCGLDRDEEALALAGQRLSSFGGR
AHLFHCRYSDFAEALNELGWDKVDGALLDIGVSSLQLDEAERGFSFYGDGPLDMRMDQNSAQPSAWHWVNRESFDKLKDC
IATLGEEPQAGRIARAIVDARQKNTIDTTGQLAALVERAYPAAWRAKARRHPATRTFQALRMAVNDELGELRRFLDQILT
WLPIGGRLAVITFHSLEDRMVKQAMRHWVEGCRCPRHIPRCICGHQPEVRILHKKPVQAGPEELAANSRAGSAKLRAVEK
IAEDSGS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=37.202380952381, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI165377202, Length=198, Percent_Identity=39.8989898989899, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786270, Length=320, Percent_Identity=43.125, Blast_Score=201, Evalue=5e-53,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=39.2857142857143, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=39.2857142857143, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_DESDA (B8IZT3)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479688.1
- ProteinModelPortal:   B8IZT3
- GeneID:   7284787
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1105
- HOGENOM:   HBG302779
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35967; Mature: 35967

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: BINDING 61-61 BINDING 95-95 BINDING 109-109 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTMSDTTEPTRHVSVLPVETLAALDPRAGGRYLDGTLGMGGHASAILSSAPGIELCGL
CCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECC
DRDEEALALAGQRLSSFGGRAHLFHCRYSDFAEALNELGWDKVDGALLDIGVSSLQLDEA
CCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCHHCCHHHCCCCHHHHHHHH
ERGFSFYGDGPLDMRMDQNSAQPSAWHWVNRESFDKLKDCIATLGEEPQAGRIARAIVDA
HCCCCEECCCCCCEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RQKNTIDTTGQLAALVERAYPAAWRAKARRHPATRTFQALRMAVNDELGELRRFLDQILT
HHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLPIGGRLAVITFHSLEDRMVKQAMRHWVEGCRCPRHIPRCICGHQPEVRILHKKPVQAG
HHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCC
PEELAANSRAGSAKLRAVEKIAEDSGS
HHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIQTMSDTTEPTRHVSVLPVETLAALDPRAGGRYLDGTLGMGGHASAILSSAPGIELCGL
CCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECC
DRDEEALALAGQRLSSFGGRAHLFHCRYSDFAEALNELGWDKVDGALLDIGVSSLQLDEA
CCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCHHCCHHHCCCCHHHHHHHH
ERGFSFYGDGPLDMRMDQNSAQPSAWHWVNRESFDKLKDCIATLGEEPQAGRIARAIVDA
HCCCCEECCCCCCEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RQKNTIDTTGQLAALVERAYPAAWRAKARRHPATRTFQALRMAVNDELGELRRFLDQILT
HHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLPIGGRLAVITFHSLEDRMVKQAMRHWVEGCRCPRHIPRCICGHQPEVRILHKKPVQAG
HHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCC
PEELAANSRAGSAKLRAVEKIAEDSGS
HHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA