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The map label for this gene is livK [C]

Identifier: 220904338

GI number: 220904338

Start: 1248228

End: 1249373

Strand: Direct

Name: livK [C]

Synonym: Ddes_1065

Alternate gene names: 220904338

Gene position: 1248228-1249373 (Clockwise)

Preceding gene: 220904337

Following gene: 220904339

Centisome position: 43.44

GC content: 57.24

Gene sequence:

>1146_bases
ATGAAAATGGGCAAATTTTTTGCGGCGCTGGCCGGGCTGGCGCTCACCTTCGGCTTTGCCGGGCAGGCTCTGGCCGCTGA
TGCCGCTCCTATCAAAATAGGCGTATACCTTCCCTTGACCGGGCAGAATGCCTTTGGCGGGCAGCTTGAGCTTGAAGGCG
TACGCCTGGCCCATAAGGAAATGCCCACCGTTCTCGGGCGTCCTGTTGATCTGGTGGTTGTGGACAACAAGTCCGACAAG
GTCGAGGCTGCCAACGCTGTAAAACGCCTTGTGGAACGTGACAAGGTTGTGGCGCTCATCGGTTCCTATGGTTCTTCTTT
GGCCATGTCTGGCGCAGAAGTGGCCGAAAAGGCCAAAGTTCCCGGCATCGGCACCTCCTGCACCAACCCCCTCGTGACCC
AGGGCAAGAAGTATTACTTCCGCGCCTGCTTCATCGACCCCTATCAGGGGGCTGCCGCGGCCACGTATGCCCGTGAAAAC
CTGGGCTTCAAAAAGGCCGCCGTGCTTATGGATATGACCAATGACTACGCTGTGGGCCTGGCCAGTTTCTTCTCCCGTTC
GTTCAAGAAGCAGGGAGGAGAAGTTGTCGGCACTCTCAAGTACAGCTCCGGCGACCAGGACTTCACGGCCCAGCTTACCG
AACTGATCTCCAAACAGCCCGACATCGTTTTCATGCCCGCCTACTTTGCTGAAGGCGCTATCATCATGAAGCAGGCCCGT
GAACTGGGTGCCAAGTTCCGCCTTATGGGCGCGGACGCCATGGATAACCCCGACACCCTCAAGCTCGGCGGCAAGGCTGC
TGAAGGCTTCCTGCACACCACCTTTCCCTATGATCCGGCTATGACCAATATGAGCGAAGCCGCCCAGCGGTTTACCGATG
CCTGGAAGGCCACCTATCCCGACAAGGAAACCAACGTTAACGGCGCTCTTGGATACAACAGCTACTTCATCATCATTGAC
GCCATCAAGCGCGCTAATTCCGCCGAGCCTCAGGCCATTGCCAAGGCTCTGGGCGAAACCAAGGACCTGTCCACGGCTCT
TGGCGTGTTGTCCATCAACGAATCTCACGATGCTGAAATGCCCGTGGGCATCATTGAGTACAAGGATGGCAAGCGCGTGT
ATGTGGGCGAAGTGACGCCCAAATAA

Upstream 100 bases:

>100_bases
TTTCTGAATTGCAGGCTATGCTCAAGTTGTTTATAGTGTGGGCCTGATGGCCGCGTCCTTGTGGCACGGCCCTGTGAAAA
CAAAAAACTGGAGGGAAAGG

Downstream 100 bases:

>100_bases
GTTGCCTTTTCCCTGCCCCGCAGCCTCTGTGGCTGCGGGGCGCTTTTCTCTTTTTTTGCCGCCGTCCGCGCGGAAAGGCC
CCTTGTGGCCTTTCTTTCGT

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKMGKFFAALAGLALTFGFAGQALAADAAPIKIGVYLPLTGQNAFGGQLELEGVRLAHKEMPTVLGRPVDLVVVDNKSDK
VEAANAVKRLVERDKVVALIGSYGSSLAMSGAEVAEKAKVPGIGTSCTNPLVTQGKKYYFRACFIDPYQGAAAATYAREN
LGFKKAAVLMDMTNDYAVGLASFFSRSFKKQGGEVVGTLKYSSGDQDFTAQLTELISKQPDIVFMPAYFAEGAIIMKQAR
ELGAKFRLMGADAMDNPDTLKLGGKAAEGFLHTTFPYDPAMTNMSEAAQRFTDAWKATYPDKETNVNGALGYNSYFIIID
AIKRANSAEPQAIAKALGETKDLSTALGVLSINESHDAEMPVGIIEYKDGKRVYVGEVTPK

Sequences:

>Translated_381_residues
MKMGKFFAALAGLALTFGFAGQALAADAAPIKIGVYLPLTGQNAFGGQLELEGVRLAHKEMPTVLGRPVDLVVVDNKSDK
VEAANAVKRLVERDKVVALIGSYGSSLAMSGAEVAEKAKVPGIGTSCTNPLVTQGKKYYFRACFIDPYQGAAAATYAREN
LGFKKAAVLMDMTNDYAVGLASFFSRSFKKQGGEVVGTLKYSSGDQDFTAQLTELISKQPDIVFMPAYFAEGAIIMKQAR
ELGAKFRLMGADAMDNPDTLKLGGKAAEGFLHTTFPYDPAMTNMSEAAQRFTDAWKATYPDKETNVNGALGYNSYFIIID
AIKRANSAEPQAIAKALGETKDLSTALGVLSINESHDAEMPVGIIEYKDGKRVYVGEVTPK
>Mature_381_residues
MKMGKFFAALAGLALTFGFAGQALAADAAPIKIGVYLPLTGQNAFGGQLELEGVRLAHKEMPTVLGRPVDLVVVDNKSDK
VEAANAVKRLVERDKVVALIGSYGSSLAMSGAEVAEKAKVPGIGTSCTNPLVTQGKKYYFRACFIDPYQGAAAATYAREN
LGFKKAAVLMDMTNDYAVGLASFFSRSFKKQGGEVVGTLKYSSGDQDFTAQLTELISKQPDIVFMPAYFAEGAIIMKQAR
ELGAKFRLMGADAMDNPDTLKLGGKAAEGFLHTTFPYDPAMTNMSEAAQRFTDAWKATYPDKETNVNGALGYNSYFIIID
AIKRANSAEPQAIAKALGETKDLSTALGVLSINESHDAEMPVGIIEYKDGKRVYVGEVTPK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=352, Percent_Identity=28.125, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI48994941, Length=355, Percent_Identity=28.169014084507, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40786; Mature: 40786

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMGKFFAALAGLALTFGFAGQALAADAAPIKIGVYLPLTGQNAFGGQLELEGVRLAHKE
CCHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEEEECCCCCCCCEEEECCCHHHHHH
MPTVLGRPVDLVVVDNKSDKVEAANAVKRLVERDKVVALIGSYGSSLAMSGAEVAEKAKV
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCHHHHHHCCC
PGIGTSCTNPLVTQGKKYYFRACFIDPYQGAAAATYARENLGFKKAAVLMDMTNDYAVGL
CCCCCCCCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHH
ASFFSRSFKKQGGEVVGTLKYSSGDQDFTAQLTELISKQPDIVFMPAYFAEGAIIMKQAR
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECHHHHCCHHHHHHHH
ELGAKFRLMGADAMDNPDTLKLGGKAAEGFLHTTFPYDPAMTNMSEAAQRFTDAWKATYP
HCCCEEEEECCCCCCCCCEEEECCCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHCCCC
DKETNVNGALGYNSYFIIIDAIKRANSAEPQAIAKALGETKDLSTALGVLSINESHDAEM
CCCCCCCEEECCCCEEEEEEEHHCCCCCCHHHHHHHHCCCHHHHHHHEEEEECCCCCCCC
PVGIIEYKDGKRVYVGEVTPK
CEEEEEECCCCEEEEECCCCC
>Mature Secondary Structure
MKMGKFFAALAGLALTFGFAGQALAADAAPIKIGVYLPLTGQNAFGGQLELEGVRLAHKE
CCHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEEEECCCCCCCCEEEECCCHHHHHH
MPTVLGRPVDLVVVDNKSDKVEAANAVKRLVERDKVVALIGSYGSSLAMSGAEVAEKAKV
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCHHHHHHCCC
PGIGTSCTNPLVTQGKKYYFRACFIDPYQGAAAATYARENLGFKKAAVLMDMTNDYAVGL
CCCCCCCCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHH
ASFFSRSFKKQGGEVVGTLKYSSGDQDFTAQLTELISKQPDIVFMPAYFAEGAIIMKQAR
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECHHHHCCHHHHHHHH
ELGAKFRLMGADAMDNPDTLKLGGKAAEGFLHTTFPYDPAMTNMSEAAQRFTDAWKATYP
HCCCEEEEECCCCCCCCCEEEECCCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHCCCC
DKETNVNGALGYNSYFIIIDAIKRANSAEPQAIAKALGETKDLSTALGVLSINESHDAEM
CCCCCCCEEECCCCEEEEEEEHHCCCCCCHHHHHHHHCCCHHHHHHHEEEEECCCCCCCC
PVGIIEYKDGKRVYVGEVTPK
CEEEEEECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]