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The map label for this gene is tilS [H]

Identifier: 220904230

GI number: 220904230

Start: 1116118

End: 1117251

Strand: Reverse

Name: tilS [H]

Synonym: Ddes_0957

Alternate gene names: 220904230

Gene position: 1117251-1116118 (Counterclockwise)

Preceding gene: 220904231

Following gene: 220904222

Centisome position: 38.88

GC content: 63.93

Gene sequence:

>1134_bases
ATGAACCAGCCCCCACGCCTGCAGGACCTTCCCCGTGCCTCTGCCCTGCTCTGCCTTGAAGTAGAGCGCTTCTGCCTGCA
TCACCTGGCTCTGCCGCGCGGCTCAAGTCTTGTGCTGGCCCTTTCCGGTGGTGCGGATTCCACAGCGCTGGCCGCCATAC
TTTCCCTGCTTGTCCCCCGGCTGCACCTGAAGCTCCGGGGCTTGAGCATCAATCATGGCTTGAGGCCCGAAGCCAAGGCT
GATGCGGCACATGCGCAAAAAACATGCAGCATCCTGGGTATGCCCTGTGCCGTGCGCGAAGCGGATGTGCAGGGCCTGGC
CGCCCGCTGCCGCATGGGCGAAGAAGAGGCCGGGCGTACCTTGCGATATGCCCTGCTTGAAGAAGAGCGACAGACGAGTG
GCGCGCACTTTATCGCCCTCGGGCACCATCGTGAAGATCTTAGCGAAGATATCCTGCTGCGGCTTGTGCGCGGCACAGGC
TGGCCTGCCCTTGGCGGCATGCCCGCGCGGGACGACAAACGCCGCCTGCTGCGCCCCCTGCTGCATTGCGCCCCCTCGGC
CCTCAAAGACCTCTTACGGCACTGCGGCCTTGACTGGTGTGAAGATGCCAGCAACCAGAGCACCATATATCGCCGCAACC
GGTTGCGACTTCAGATACTGCCGCTGCTGCGGACGGAAAACCCCGCTCTGGACCGCAGCCTGGGCCACCTGTGGCAACTT
GCCGATATGGACAGGACCTATTGGCAGGAAACCCTGGATGCCGCACTGCGCGACAATCCCTGGCATATGGAAGAAGCTGC
GCATGACGCACAGGCTGGGCTGAACGGGCAGGCGCGCACGGCCCTCACCCTGCCCCGCGCCCTGCTACGGTCGCTGCCCG
CTGCGGCCCGTTTGCGCCTTTACATGCGGGCAGTGCAGCACCTGTGTTCCGCACCTGATGCGGAAGACGATGGCTTGCCA
GACAGCATGGCACAGCAAACAGCCACCACAGGGCAGGCCAGAGCGGCAACCTTGCTGGCTCTGGACCATGCGCTGGAACA
GGGGCGCGGCAATACGCTTTTTCAGTTGCCTGGGGGCATTGAGGCTCACCTCAAGGGTGGGAGTGTCCGCTTTTTGCGCT
CCGGCCCGCTATGA

Upstream 100 bases:

>100_bases
CCATCCCCCACAACATATTTCAAACGTTCTCTATTTTAAAAGGCTCCCCAGCTGTTGACGCCAAACAGGTACAGCCCGCA
AACAAGGCTCGAGCACAAGC

Downstream 100 bases:

>100_bases
GGCGGCTCCGCTGGAACACTTTGCTCTTGATAATATAAATCTTATAATTAATCAGAAATATTATGCGCATCAGGCATTCG
TGGATGGCGTTGCGCGCCGG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNQPPRLQDLPRASALLCLEVERFCLHHLALPRGSSLVLALSGGADSTALAAILSLLVPRLHLKLRGLSINHGLRPEAKA
DAAHAQKTCSILGMPCAVREADVQGLAARCRMGEEEAGRTLRYALLEEERQTSGAHFIALGHHREDLSEDILLRLVRGTG
WPALGGMPARDDKRRLLRPLLHCAPSALKDLLRHCGLDWCEDASNQSTIYRRNRLRLQILPLLRTENPALDRSLGHLWQL
ADMDRTYWQETLDAALRDNPWHMEEAAHDAQAGLNGQARTALTLPRALLRSLPAAARLRLYMRAVQHLCSAPDAEDDGLP
DSMAQQTATTGQARAATLLALDHALEQGRGNTLFQLPGGIEAHLKGGSVRFLRSGPL

Sequences:

>Translated_377_residues
MNQPPRLQDLPRASALLCLEVERFCLHHLALPRGSSLVLALSGGADSTALAAILSLLVPRLHLKLRGLSINHGLRPEAKA
DAAHAQKTCSILGMPCAVREADVQGLAARCRMGEEEAGRTLRYALLEEERQTSGAHFIALGHHREDLSEDILLRLVRGTG
WPALGGMPARDDKRRLLRPLLHCAPSALKDLLRHCGLDWCEDASNQSTIYRRNRLRLQILPLLRTENPALDRSLGHLWQL
ADMDRTYWQETLDAALRDNPWHMEEAAHDAQAGLNGQARTALTLPRALLRSLPAAARLRLYMRAVQHLCSAPDAEDDGLP
DSMAQQTATTGQARAATLLALDHALEQGRGNTLFQLPGGIEAHLKGGSVRFLRSGPL
>Mature_377_residues
MNQPPRLQDLPRASALLCLEVERFCLHHLALPRGSSLVLALSGGADSTALAAILSLLVPRLHLKLRGLSINHGLRPEAKA
DAAHAQKTCSILGMPCAVREADVQGLAARCRMGEEEAGRTLRYALLEEERQTSGAHFIALGHHREDLSEDILLRLVRGTG
WPALGGMPARDDKRRLLRPLLHCAPSALKDLLRHCGLDWCEDASNQSTIYRRNRLRLQILPLLRTENPALDRSLGHLWQL
ADMDRTYWQETLDAALRDNPWHMEEAAHDAQAGLNGQARTALTLPRALLRSLPAAARLRLYMRAVQHLCSAPDAEDDGLP
DSMAQQTATTGQARAATLLALDHALEQGRGNTLFQLPGGIEAHLKGGSVRFLRSGPL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=195, Percent_Identity=31.7948717948718, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 41372; Mature: 41372

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPPRLQDLPRASALLCLEVERFCLHHLALPRGSSLVLALSGGADSTALAAILSLLVPR
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LHLKLRGLSINHGLRPEAKADAAHAQKTCSILGMPCAVREADVQGLAARCRMGEEEAGRT
HHHEEECEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCHH
LRYALLEEERQTSGAHFIALGHHREDLSEDILLRLVRGTGWPALGGMPARDDKRRLLRPL
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LHCAPSALKDLLRHCGLDWCEDASNQSTIYRRNRLRLQILPLLRTENPALDRSLGHLWQL
HHHCHHHHHHHHHHCCCCHHHCCCCCCHHHHHCCCEEEEEEHHCCCCCHHHHHHHHHHHH
ADMDRTYWQETLDAALRDNPWHMEEAAHDAQAGLNGQARTALTLPRALLRSLPAAARLRL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
YMRAVQHLCSAPDAEDDGLPDSMAQQTATTGQARAATLLALDHALEQGRGNTLFQLPGGI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EAHLKGGSVRFLRSGPL
EEEECCCCEEEEECCCC
>Mature Secondary Structure
MNQPPRLQDLPRASALLCLEVERFCLHHLALPRGSSLVLALSGGADSTALAAILSLLVPR
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LHLKLRGLSINHGLRPEAKADAAHAQKTCSILGMPCAVREADVQGLAARCRMGEEEAGRT
HHHEEECEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCHH
LRYALLEEERQTSGAHFIALGHHREDLSEDILLRLVRGTGWPALGGMPARDDKRRLLRPL
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LHCAPSALKDLLRHCGLDWCEDASNQSTIYRRNRLRLQILPLLRTENPALDRSLGHLWQL
HHHCHHHHHHHHHHCCCCHHHCCCCCCHHHHHCCCEEEEEEHHCCCCCHHHHHHHHHHHH
ADMDRTYWQETLDAALRDNPWHMEEAAHDAQAGLNGQARTALTLPRALLRSLPAAARLRL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
YMRAVQHLCSAPDAEDDGLPDSMAQQTATTGQARAATLLALDHALEQGRGNTLFQLPGGI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EAHLKGGSVRFLRSGPL
EEEECCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA