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The map label for this gene is ddl

Identifier: 220904145

GI number: 220904145

Start: 1021124

End: 1022044

Strand: Direct

Name: ddl

Synonym: Ddes_0872

Alternate gene names: 220904145

Gene position: 1021124-1022044 (Clockwise)

Preceding gene: 220904144

Following gene: 220904146

Centisome position: 35.54

GC content: 60.04

Gene sequence:

>921_bases
ATGAAGATTCTTTTGATTGCGGGCGGCTGGTCTCCTGAACGACAGGTATCACTGAACGGCGCAAGGGCCATGGTTCCTGC
CCTGGAGGCGCGTGGTCATGAGGTGACATTTTTTGACCTTCTTGAAAATTTTAACGGTCTGCTTGCCGCCGCCGAGCAGC
ATGATTTCGCCCTCATCAACCTGCACGGCGCTCCGGGTGAAGATGGCCTTGTGCAGGCCATGCTGGATCAGGTGGGCTGT
CCCTACCAGGGGGCCGGTCCCGCAGGTTCTTTCCTGGCGCTCAACAAGTGCGCGGCGAAACAGATATTTCGCCGGGCGGG
GCTGCCCACGGCGGACTGGGACTTTGTGCCCGCGTTCCCCGGAAAAGACTGGCAACCCCGGCTGCCATATCCCCTGTTTG
TCAAGAGTAATACGGGTGGGTCCTCACTGCGCCTCGGCCGCGCGCGCAACCGTGCCGAGCTTGACGATATTATGGGCCAG
ATATTCGCTGCGGGCGAAGAAGTCATCATGGAACCCGTGCTGCCGGGCCGTGAAGTGACGTGCGGTATTCTGGGTGAAGA
TCCTCTGCCGCCCATCCTTATTGAGCCGGTAGCCGGGGATTTTTTCAATTACGAGAGCAAGTACGAACAGGACGGCGCGC
GCGAGCTATGCCCGGCCCCCATTGGTGACGAACTGACCGCACGTGTACAGGAACTGACCCTCGCCGCACACAGGGCGCTT
GGCCTCAGGGGGTACAGCAGGGCAGACTTTATCCTCGGGCCGGACGACAGCCTTACCCTGCTGGAGGTCAATACCTTGCC
CGGCATGACCGCCACCAGCCTTGTACCGCGCGAGGCCAGAACCATCGGCCTTGATTTCGGCCAGCTGCTTGAGCGCCTTA
TGGAACTGGGCCTCGCCGACTGTAAAAAAAAACAGATGTAG

Upstream 100 bases:

>100_bases
TATGGGCACAGTGAGGCGGCGCGTGAAGGTATTTGCGCCGCCTTCCGCCAGGGGAAAAATATTGAAAACGCTCTTTCGGA
GCAACTGGGATGGACACTGA

Downstream 100 bases:

>100_bases
CGCAGCAGTAAAGGGTCTTCGTGCGTGGTGCAAACCCGCACCGCCTCTCCATTCCACCGTGGTAAGCATGGCGGGCCGGA
GGGGTTTTTTATCAGCATGC

Product: D-alanine/D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKILLIAGGWSPERQVSLNGARAMVPALEARGHEVTFFDLLENFNGLLAAAEQHDFALINLHGAPGEDGLVQAMLDQVGC
PYQGAGPAGSFLALNKCAAKQIFRRAGLPTADWDFVPAFPGKDWQPRLPYPLFVKSNTGGSSLRLGRARNRAELDDIMGQ
IFAAGEEVIMEPVLPGREVTCGILGEDPLPPILIEPVAGDFFNYESKYEQDGARELCPAPIGDELTARVQELTLAAHRAL
GLRGYSRADFILGPDDSLTLLEVNTLPGMTATSLVPREARTIGLDFGQLLERLMELGLADCKKKQM

Sequences:

>Translated_306_residues
MKILLIAGGWSPERQVSLNGARAMVPALEARGHEVTFFDLLENFNGLLAAAEQHDFALINLHGAPGEDGLVQAMLDQVGC
PYQGAGPAGSFLALNKCAAKQIFRRAGLPTADWDFVPAFPGKDWQPRLPYPLFVKSNTGGSSLRLGRARNRAELDDIMGQ
IFAAGEEVIMEPVLPGREVTCGILGEDPLPPILIEPVAGDFFNYESKYEQDGARELCPAPIGDELTARVQELTLAAHRAL
GLRGYSRADFILGPDDSLTLLEVNTLPGMTATSLVPREARTIGLDFGQLLERLMELGLADCKKKQM
>Mature_306_residues
MKILLIAGGWSPERQVSLNGARAMVPALEARGHEVTFFDLLENFNGLLAAAEQHDFALINLHGAPGEDGLVQAMLDQVGC
PYQGAGPAGSFLALNKCAAKQIFRRAGLPTADWDFVPAFPGKDWQPRLPYPLFVKSNTGGSSLRLGRARNRAELDDIMGQ
IFAAGEEVIMEPVLPGREVTCGILGEDPLPPILIEPVAGDFFNYESKYEQDGARELCPAPIGDELTARVQELTLAAHRAL
GLRGYSRADFILGPDDSLTLLEVNTLPGMTATSLVPREARTIGLDFGQLLERLMELGLADCKKKQM

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=304, Percent_Identity=38.8157894736842, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=27.8735632183908, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_DESDA (B8IZ52)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479457.1
- GeneID:   7284548
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0872
- HOGENOM:   HBG427092
- ProtClustDB:   CLSK704556
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33138; Mature: 33138

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLIAGGWSPERQVSLNGARAMVPALEARGHEVTFFDLLENFNGLLAAAEQHDFALIN
CEEEEEECCCCCCCEEECCCCHHHHHHHHCCCCEEEHHHHHHCCCCEEEECCCCCEEEEE
LHGAPGEDGLVQAMLDQVGCPYQGAGPAGSFLALNKCAAKQIFRRAGLPTADWDFVPAFP
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCC
GKDWQPRLPYPLFVKSNTGGSSLRLGRARNRAELDDIMGQIFAAGEEVIMEPVLPGREVT
CCCCCCCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEE
CGILGEDPLPPILIEPVAGDFFNYESKYEQDGARELCPAPIGDELTARVQELTLAAHRAL
EEECCCCCCCCEEEECCCCCHHCCCHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHH
GLRGYSRADFILGPDDSLTLLEVNTLPGMTATSLVPREARTIGLDFGQLLERLMELGLAD
CCCCCCCCCEEECCCCCEEEEEEECCCCCCHHHCCCCCHHEECCCHHHHHHHHHHCCHHH
CKKKQM
HHHCCC
>Mature Secondary Structure
MKILLIAGGWSPERQVSLNGARAMVPALEARGHEVTFFDLLENFNGLLAAAEQHDFALIN
CEEEEEECCCCCCCEEECCCCHHHHHHHHCCCCEEEHHHHHHCCCCEEEECCCCCEEEEE
LHGAPGEDGLVQAMLDQVGCPYQGAGPAGSFLALNKCAAKQIFRRAGLPTADWDFVPAFP
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCC
GKDWQPRLPYPLFVKSNTGGSSLRLGRARNRAELDDIMGQIFAAGEEVIMEPVLPGREVT
CCCCCCCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEE
CGILGEDPLPPILIEPVAGDFFNYESKYEQDGARELCPAPIGDELTARVQELTLAAHRAL
EEECCCCCCCCEEEECCCCCHHCCCHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHH
GLRGYSRADFILGPDDSLTLLEVNTLPGMTATSLVPREARTIGLDFGQLLERLMELGLAD
CCCCCCCCCEEECCCCCEEEEEEECCCCCCHHHCCCCCHHEECCCHHHHHHHHHHCCHHH
CKKKQM
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA