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The map label for this gene is kinE [H]

Identifier: 220904087

GI number: 220904087

Start: 952510

End: 954273

Strand: Direct

Name: kinE [H]

Synonym: Ddes_0813

Alternate gene names: 220904087

Gene position: 952510-954273 (Clockwise)

Preceding gene: 220904086

Following gene: 220904088

Centisome position: 33.15

GC content: 60.32

Gene sequence:

>1764_bases
ATGTTCGCCGCCATAAAAAACGCCTTTGCCCACCTGCTGGAAGTGCCCGAGGCCGTGTCTCCGGCCCGGTACCGCTCTCT
GCGTCGGCTTATGACCATACTTATGGTGGCCGTATCGGTCACGCCTCTGCTTCTGTTGTCCGGCATAAGCCACATGCAGT
ACACACGGACCCTCGAGCGTGAAATGGAAAGCCCCGTTTACGCGCTGGCCCGCAAGTCGCAGGCGGCGCTGGAGCTTTAT
CTCGGTGAAAGGGAATCCACGGTGAGCTTTATCGCCCATGCCTATTCCTACAAGGACCTGGCGGACGAGCGCACCCTGAA
CCGCGTGTTTTTGTCGCTGCGCAGTGAGTTTCAGGGCTTTGTGGACATGGGGCTGGTCAACTCCGAGGGCCTGCAGATTT
CCTATGTGGGACCGTACAAGCTCAGGGGCGCGGACTATGCGGGCAAGCCCTGGCTGCGTGAAACGGAAATCAAAAGCCGG
TATATGAGCAACGTGTTTCTCGGTTATCGCGGCTATCCGCATATGGTCATAGCCGTTCACAGGATGGAGGAAAGCGGGCT
TTCATGGACCCTGCGCGTGGCCGTGGACACGCTGCGGCTGCAGCAGGTGCTGTCCTCCGTAGGCCCGGAGCAGGAAACCG
ATGTTTTTCTTGTGGACAGGGAAGGAGTGCTGCAAACAGATTCCCGCCTGTACGGCAAGGCGCTGGATTCCTGTCCGTTG
CCGCTGCCGCCGGCCACGGCGGAAACGCTCGTTCGCAACGTCACCGAGCCATCGGGACGCAAGCTTATGGTGGCCTCCTG
CGCACTGGCCGGTACGGATTTCATTCTGCTGGCAGTCAAGCCCGAGGCCGAGGCCTTTCGCCCCTGGACAGCCCTGCGCA
CCGAATTGCTGCTTGTGCTGTGCGGCGGTATCGCGCTTATTGTTCTGGTATCGCACCTGCTTATGAAGCAGCTTGTCAAC
AGGCTGCAGGCCAGCGATGAGCGCCGGGTAGCCATTTTCGCCCAGATGGAGCACAATCAGAAGCTCTCGTCCATCGGGCG
GCTGGCGGCGGGTGTGGCCCACGAGGTCAACAATCCCCTTGCCGTCATTTACGAAAAGGCGGGCCTTGCCCGCGACCTGC
TGAGCATGGGCAAGGTTTGCGGCGAAAGCAGGGACAGGGAGAGGCTGAACGGTCTGCTCGAAGGCATAGAAAGCACAGTG
GAGCGGGCACGCAGTATTACCCACCGCCTGCTGGGTTTCGCCCGCCGGATGGAAGCCAACCGCCAGAGCCTGCACATTGA
GGAGGTCATCACCGAGACCCTGTCTTTCCTTGAGCGAGAAGCCAAGAACCGGGGCGTAACGCTGGAGATGGATCTGGCCG
AGGACCTGCCCGAGATTGTTTCGGACAGGGGCCAGTTGCAGCAGGTATTTCTGAATATCGTGGGCAATGCCCTGGATGCC
GTGACCGGCAGCGGGCAGGCAGGCGCATCTGCCGACGGGCAGGGGCGCTTTGTAAAAATACGCTGTGTTCCATCGGGACA
GTGCGCGCTTGAAGTGACCGTGCGTGATAACGGCAAAGGCATGCCTCGAGAGGTGCTGCGGCACATTTTCGAGCCGTTTT
ATTCCACCAAGAAAGATAAGGGTACAGGGCTTGGCATGTTCATCACCTACGGCATCGTGCGTCGGCTCGGCGGCGATATC
CGTGTGGACAGTGAAGAAGGGCGCGGCAGTACCGTGCGCGTGACCCTGCCTCTGGCTCCGCCTGACGGCTCTGTGGAGGT
TTAG

Upstream 100 bases:

>100_bases
CCCTCATGACGGCGCATGCCGGCGCCTGATCAGGATATGAAGGGCTGCACCGTGAAAAAGCACGTTTGAAGCTTGCGCCA
GCGCGCAGCCCGGAGGAAGC

Downstream 100 bases:

>100_bases
TAATGGCTTTGCGCATACTGCTGGCTGATGACGAAAAGGATTTTGTGGAAACTCTGGCCGAAAGGCTTGAACTGCGCGGG
CACAGCGTGCGCGTGGTTTA

Product: histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MFAAIKNAFAHLLEVPEAVSPARYRSLRRLMTILMVAVSVTPLLLLSGISHMQYTRTLEREMESPVYALARKSQAALELY
LGERESTVSFIAHAYSYKDLADERTLNRVFLSLRSEFQGFVDMGLVNSEGLQISYVGPYKLRGADYAGKPWLRETEIKSR
YMSNVFLGYRGYPHMVIAVHRMEESGLSWTLRVAVDTLRLQQVLSSVGPEQETDVFLVDREGVLQTDSRLYGKALDSCPL
PLPPATAETLVRNVTEPSGRKLMVASCALAGTDFILLAVKPEAEAFRPWTALRTELLLVLCGGIALIVLVSHLLMKQLVN
RLQASDERRVAIFAQMEHNQKLSSIGRLAAGVAHEVNNPLAVIYEKAGLARDLLSMGKVCGESRDRERLNGLLEGIESTV
ERARSITHRLLGFARRMEANRQSLHIEEVITETLSFLEREAKNRGVTLEMDLAEDLPEIVSDRGQLQQVFLNIVGNALDA
VTGSGQAGASADGQGRFVKIRCVPSGQCALEVTVRDNGKGMPREVLRHIFEPFYSTKKDKGTGLGMFITYGIVRRLGGDI
RVDSEEGRGSTVRVTLPLAPPDGSVEV

Sequences:

>Translated_587_residues
MFAAIKNAFAHLLEVPEAVSPARYRSLRRLMTILMVAVSVTPLLLLSGISHMQYTRTLEREMESPVYALARKSQAALELY
LGERESTVSFIAHAYSYKDLADERTLNRVFLSLRSEFQGFVDMGLVNSEGLQISYVGPYKLRGADYAGKPWLRETEIKSR
YMSNVFLGYRGYPHMVIAVHRMEESGLSWTLRVAVDTLRLQQVLSSVGPEQETDVFLVDREGVLQTDSRLYGKALDSCPL
PLPPATAETLVRNVTEPSGRKLMVASCALAGTDFILLAVKPEAEAFRPWTALRTELLLVLCGGIALIVLVSHLLMKQLVN
RLQASDERRVAIFAQMEHNQKLSSIGRLAAGVAHEVNNPLAVIYEKAGLARDLLSMGKVCGESRDRERLNGLLEGIESTV
ERARSITHRLLGFARRMEANRQSLHIEEVITETLSFLEREAKNRGVTLEMDLAEDLPEIVSDRGQLQQVFLNIVGNALDA
VTGSGQAGASADGQGRFVKIRCVPSGQCALEVTVRDNGKGMPREVLRHIFEPFYSTKKDKGTGLGMFITYGIVRRLGGDI
RVDSEEGRGSTVRVTLPLAPPDGSVEV
>Mature_587_residues
MFAAIKNAFAHLLEVPEAVSPARYRSLRRLMTILMVAVSVTPLLLLSGISHMQYTRTLEREMESPVYALARKSQAALELY
LGERESTVSFIAHAYSYKDLADERTLNRVFLSLRSEFQGFVDMGLVNSEGLQISYVGPYKLRGADYAGKPWLRETEIKSR
YMSNVFLGYRGYPHMVIAVHRMEESGLSWTLRVAVDTLRLQQVLSSVGPEQETDVFLVDREGVLQTDSRLYGKALDSCPL
PLPPATAETLVRNVTEPSGRKLMVASCALAGTDFILLAVKPEAEAFRPWTALRTELLLVLCGGIALIVLVSHLLMKQLVN
RLQASDERRVAIFAQMEHNQKLSSIGRLAAGVAHEVNNPLAVIYEKAGLARDLLSMGKVCGESRDRERLNGLLEGIESTV
ERARSITHRLLGFARRMEANRQSLHIEEVITETLSFLEREAKNRGVTLEMDLAEDLPEIVSDRGQLQQVFLNIVGNALDA
VTGSGQAGASADGQGRFVKIRCVPSGQCALEVTVRDNGKGMPREVLRHIFEPFYSTKKDKGTGLGMFITYGIVRRLGGDI
RVDSEEGRGSTVRVTLPLAPPDGSVEV

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=244, Percent_Identity=32.7868852459016, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=30.4878048780488, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789149, Length=244, Percent_Identity=27.4590163934426, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790300, Length=268, Percent_Identity=28.7313432835821, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=24.1525423728814, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790346, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=130, Percent_Identity=33.0769230769231, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64930; Mature: 64930

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAAIKNAFAHLLEVPEAVSPARYRSLRRLMTILMVAVSVTPLLLLSGISHMQYTRTLER
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMESPVYALARKSQAALELYLGERESTVSFIAHAYSYKDLADERTLNRVFLSLRSEFQGF
HHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDMGLVNSEGLQISYVGPYKLRGADYAGKPWLRETEIKSRYMSNVFLGYRGYPHMVIAVH
HHHCEECCCCEEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RMEESGLSWTLRVAVDTLRLQQVLSSVGPEQETDVFLVDREGVLQTDSRLYGKALDSCPL
EHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCC
PLPPATAETLVRNVTEPSGRKLMVASCALAGTDFILLAVKPEAEAFRPWTALRTELLLVL
CCCCCHHHHHHHHCCCCCCCEEEEEEHHHCCCCEEEEEECCCCHHHCCHHHHHHHHHHHH
CGGIALIVLVSHLLMKQLVNRLQASDERRVAIFAQMEHNQKLSSIGRLAAGVAHEVNNPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHCHHHHHHHHHHHHHHHHCCCCE
AVIYEKAGLARDLLSMGKVCGESRDRERLNGLLEGIESTVERARSITHRLLGFARRMEAN
EEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RQSLHIEEVITETLSFLEREAKNRGVTLEMDLAEDLPEIVSDRGQLQQVFLNIVGNALDA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
VTGSGQAGASADGQGRFVKIRCVPSGQCALEVTVRDNGKGMPREVLRHIFEPFYSTKKDK
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
GTGLGMFITYGIVRRLGGDIRVDSEEGRGSTVRVTLPLAPPDGSVEV
CCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MFAAIKNAFAHLLEVPEAVSPARYRSLRRLMTILMVAVSVTPLLLLSGISHMQYTRTLER
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMESPVYALARKSQAALELYLGERESTVSFIAHAYSYKDLADERTLNRVFLSLRSEFQGF
HHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDMGLVNSEGLQISYVGPYKLRGADYAGKPWLRETEIKSRYMSNVFLGYRGYPHMVIAVH
HHHCEECCCCEEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RMEESGLSWTLRVAVDTLRLQQVLSSVGPEQETDVFLVDREGVLQTDSRLYGKALDSCPL
EHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCC
PLPPATAETLVRNVTEPSGRKLMVASCALAGTDFILLAVKPEAEAFRPWTALRTELLLVL
CCCCCHHHHHHHHCCCCCCCEEEEEEHHHCCCCEEEEEECCCCHHHCCHHHHHHHHHHHH
CGGIALIVLVSHLLMKQLVNRLQASDERRVAIFAQMEHNQKLSSIGRLAAGVAHEVNNPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHCHHHHHHHHHHHHHHHHCCCCE
AVIYEKAGLARDLLSMGKVCGESRDRERLNGLLEGIESTVERARSITHRLLGFARRMEAN
EEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RQSLHIEEVITETLSFLEREAKNRGVTLEMDLAEDLPEIVSDRGQLQQVFLNIVGNALDA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
VTGSGQAGASADGQGRFVKIRCVPSGQCALEVTVRDNGKGMPREVLRHIFEPFYSTKKDK
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
GTGLGMFITYGIVRRLGGDIRVDSEEGRGSTVRVTLPLAPPDGSVEV
CCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]