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The map label for this gene is pleD [H]

Identifier: 220904032

GI number: 220904032

Start: 891622

End: 892644

Strand: Direct

Name: pleD [H]

Synonym: Ddes_0758

Alternate gene names: 220904032

Gene position: 891622-892644 (Clockwise)

Preceding gene: 220904031

Following gene: 220904037

Centisome position: 31.03

GC content: 55.23

Gene sequence:

>1023_bases
ATGGATGATCCTGCGAATACCGGGGCCGCGCTCTCAGAGGACGTGGCCCTTGCGACCGAAGTTATAGGCCATGTGCATTC
CATCCTGACCGGTGTTTCCGTCAGTGAAGGCCCCCTGCCTGACAGGCTGCGGCAACTGGAAGGGATGGAGAACCTGCACA
ATCTGATATGGGGTATACGCTCCCTCGCCCACAATCTGGGCAAGGGCGACCTGCATTACATGTCCAAGCAGCGGGGCTTT
GTTATTGGTTCTCTCAAGGCCCTTCAGTCCGATCTGCGCCATCTTACGTGGCAGGCCCAGTGCATCGCCAAAGGCGAATA
CCAGCACAGGATCAACTTTTTGGGCGACTTTTCTACCGCATTCAACAAGATGGTGGAAGAACTGGACAAAAACGTTTCAG
AACTGACCCGTCTTTCTCATAAATACAAGCAGCTCTCTTTTATAGACCCTCTTACCGGGCTGCTTAACCGTCGTGCATTC
ATGGTCAAGGCCGAAAAAACGCTGGAACTCCTTCGTGAGGAAGGGTTGCCCGCCAGTGTTCTTCTTGCAGATATTGACCA
CTTCAAAGCCGTCAATGATACCTACGGGCATGCCTGCGGCGATGAGGTGCTCGTGCGCTTCAGCCGTTGCATCAGCTCTT
TTTTGCGTGAACAGGATTTGTGCTGCCGCTACGGCGGTGAAGAGTTTGTCATACTTCTTGCGGGTGAAGAACTGGCAGAA
GCATGCGAGCTGGGCGAAAAAATACGTTCGGCTGTGGAGCAGACCGTTATGGATGAAGCCGCCATGCTTTACTGCATCCA
GGCCCGCTCCAGAGAGGGGCAGGCGTCCATCCCGCATCCCGTGCCCCTGCGGATAACTGTGAGCCTTGGCGTCAGCAGGC
TGTGCCGGGACAATGTATCCTGCTCTCTCTTGCAACAGCTTGAAGCCGGCATCGCCGAGGCCGACAGATGCCTGTACCGG
GCCAAGTACAATGGACGCAACTCTGTGGTGTGCCAGGATGCGCCCTCTTTTCTTCAGGAATAG

Upstream 100 bases:

>100_bases
CCCGCCACTGGTTTGGCACTGCGCCTGTCCGCTGTGTCCGTGTTTCCGGTCCTGAGGGATATTCCCTCCGGCGGGTTCAA
GATAACCGGTAGTAAATACT

Downstream 100 bases:

>100_bases
CCTGCATATTGCCCGCCGTGTTACGGCGCGCTGCACCCTCGTGCAGCGTTAGGGGTATTTAAATTTTCTTAACGCTAGCA
ATTAACGCTTCAAATGATTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MDDPANTGAALSEDVALATEVIGHVHSILTGVSVSEGPLPDRLRQLEGMENLHNLIWGIRSLAHNLGKGDLHYMSKQRGF
VIGSLKALQSDLRHLTWQAQCIAKGEYQHRINFLGDFSTAFNKMVEELDKNVSELTRLSHKYKQLSFIDPLTGLLNRRAF
MVKAEKTLELLREEGLPASVLLADIDHFKAVNDTYGHACGDEVLVRFSRCISSFLREQDLCCRYGGEEFVILLAGEELAE
ACELGEKIRSAVEQTVMDEAAMLYCIQARSREGQASIPHPVPLRITVSLGVSRLCRDNVSCSLLQQLEAGIAEADRCLYR
AKYNGRNSVVCQDAPSFLQE

Sequences:

>Translated_340_residues
MDDPANTGAALSEDVALATEVIGHVHSILTGVSVSEGPLPDRLRQLEGMENLHNLIWGIRSLAHNLGKGDLHYMSKQRGF
VIGSLKALQSDLRHLTWQAQCIAKGEYQHRINFLGDFSTAFNKMVEELDKNVSELTRLSHKYKQLSFIDPLTGLLNRRAF
MVKAEKTLELLREEGLPASVLLADIDHFKAVNDTYGHACGDEVLVRFSRCISSFLREQDLCCRYGGEEFVILLAGEELAE
ACELGEKIRSAVEQTVMDEAAMLYCIQARSREGQASIPHPVPLRITVSLGVSRLCRDNVSCSLLQQLEAGIAEADRCLYR
AKYNGRNSVVCQDAPSFLQE
>Mature_340_residues
MDDPANTGAALSEDVALATEVIGHVHSILTGVSVSEGPLPDRLRQLEGMENLHNLIWGIRSLAHNLGKGDLHYMSKQRGF
VIGSLKALQSDLRHLTWQAQCIAKGEYQHRINFLGDFSTAFNKMVEELDKNVSELTRLSHKYKQLSFIDPLTGLLNRRAF
MVKAEKTLELLREEGLPASVLLADIDHFKAVNDTYGHACGDEVLVRFSRCISSFLREQDLCCRYGGEEFVILLAGEELAE
ACELGEKIRSAVEQTVMDEAAMLYCIQARSREGQASIPHPVPLRITVSLGVSRLCRDNVSCSLLQQLEAGIAEADRCLYR
AKYNGRNSVVCQDAPSFLQE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=202, Percent_Identity=38.1188118811881, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787262, Length=195, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081881, Length=230, Percent_Identity=32.1739130434783, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI145693134, Length=220, Percent_Identity=30.9090909090909, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=194, Percent_Identity=32.9896907216495, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=179, Percent_Identity=33.5195530726257, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786584, Length=200, Percent_Identity=29, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=209, Percent_Identity=30.622009569378, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788956, Length=126, Percent_Identity=32.5396825396825, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=112, Percent_Identity=32.1428571428571, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788381, Length=205, Percent_Identity=27.3170731707317, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=107, Percent_Identity=30.8411214953271, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787056, Length=201, Percent_Identity=25.8706467661692, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 37791; Mature: 37791

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDPANTGAALSEDVALATEVIGHVHSILTGVSVSEGPLPDRLRQLEGMENLHNLIWGIR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLAHNLGKGDLHYMSKQRGFVIGSLKALQSDLRHLTWQAQCIAKGEYQHRINFLGDFSTA
HHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FNKMVEELDKNVSELTRLSHKYKQLSFIDPLTGLLNRRAFMVKAEKTLELLREEGLPASV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHCCCCHHH
LLADIDHFKAVNDTYGHACGDEVLVRFSRCISSFLREQDLCCRYGGEEFVILLAGEELAE
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHH
ACELGEKIRSAVEQTVMDEAAMLYCIQARSREGQASIPHPVPLRITVSLGVSRLCRDNVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCH
CSLLQQLEAGIAEADRCLYRAKYNGRNSVVCQDAPSFLQE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHCC
>Mature Secondary Structure
MDDPANTGAALSEDVALATEVIGHVHSILTGVSVSEGPLPDRLRQLEGMENLHNLIWGIR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLAHNLGKGDLHYMSKQRGFVIGSLKALQSDLRHLTWQAQCIAKGEYQHRINFLGDFSTA
HHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FNKMVEELDKNVSELTRLSHKYKQLSFIDPLTGLLNRRAFMVKAEKTLELLREEGLPASV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHCCCCHHH
LLADIDHFKAVNDTYGHACGDEVLVRFSRCISSFLREQDLCCRYGGEEFVILLAGEELAE
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHH
ACELGEKIRSAVEQTVMDEAAMLYCIQARSREGQASIPHPVPLRITVSLGVSRLCRDNVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCH
CSLLQQLEAGIAEADRCLYRAKYNGRNSVVCQDAPSFLQE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA