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The map label for this gene is thiDE [H]

Identifier: 220903973

GI number: 220903973

Start: 829640

End: 830449

Strand: Reverse

Name: thiDE [H]

Synonym: Ddes_0699

Alternate gene names: 220903973

Gene position: 830449-829640 (Counterclockwise)

Preceding gene: 220903979

Following gene: 220903972

Centisome position: 28.9

GC content: 60.25

Gene sequence:

>810_bases
ATGCAGACTCCTCCCAATATCCTGACAATCGCCGGTTCCGATTCCGGCGGCGGCGCGGGCATTCAGGCCGACCTGAAAAC
CATTATGGCCTTGGGCGGCTACGGCATGAGTGTCATCGCCGCGCTCACTGCCCAGAACGGTCAGGGCGTCACGGGCATAC
ACGCCCCGGACCCGGACTTTGTAGCCCTGCAGCTCTGCACCGTGCTTGACGGTTTTCCTGTAGCGGCCGCGAAAACAGGC
ATGCTGTTTTCAGCCCCCATCATCAAGGCCATTGCCCCCATACTGCGCAAGCGCGACTTTCCGCTTGTGGTAGACCCGGT
TTCCATAAGCCAGAGCGGCAGCGTTCTTCTGCAGGAAGACGCGGTAAAAGCACTTGTACAGGAAATCCTGCCCGGCTGCG
ATCTGCTGACGCCCAACAGGCCCGAAGCGGAAATGCTCACCAACATGACCATTGATAATCTTGACGACGCGGCGGCGGCC
GGTGAAAAACTGCTGCAAATGGGCGCACGGGCCGTTCTTATCAAGGGCGGTCATATGGAAAGCAATGTGGTTGTCACCGA
CTGCCTGTGCATGGAGGGACAAGCTCCCAAACACCTGCCGCAGGCCAAGGTAGAAACCGTCAACAACCACGGCACGGGCT
GCACCCTTTCCGCCGCCATTGCCACCGGCCTTGGCAAGGGGCTGCCCCTGCAGGTGGCGGTAACCCGCGCACAGGAGTTT
CTCAATCTTGCCCTGCGCCGCAGCTACAGCCCCGGCAACGGATGCGGTCCTGTCAATCACTCGGCCGGGTTGAACCTTCA
GGGTATTTAA

Upstream 100 bases:

>100_bases
ATACGGCCATACGATGCGGACGCGGGCCGCACATTGCATCAGTCAACAGGGAAGGCTATAGTATACCCGGCTCACATAAA
ATACATACCTAGAGGGCAAG

Downstream 100 bases:

>100_bases
ACTTCCTTAAAGATAAAATGCCCTGAAACCGGCGCATGCCGCAAAAAAACAGTTGAAGGCGCTTGAACGCCCGCTGCGGC
AGACTGCACTTTTCGTGGCA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MQTPPNILTIAGSDSGGGAGIQADLKTIMALGGYGMSVIAALTAQNGQGVTGIHAPDPDFVALQLCTVLDGFPVAAAKTG
MLFSAPIIKAIAPILRKRDFPLVVDPVSISQSGSVLLQEDAVKALVQEILPGCDLLTPNRPEAEMLTNMTIDNLDDAAAA
GEKLLQMGARAVLIKGGHMESNVVVTDCLCMEGQAPKHLPQAKVETVNNHGTGCTLSAAIATGLGKGLPLQVAVTRAQEF
LNLALRRSYSPGNGCGPVNHSAGLNLQGI

Sequences:

>Translated_269_residues
MQTPPNILTIAGSDSGGGAGIQADLKTIMALGGYGMSVIAALTAQNGQGVTGIHAPDPDFVALQLCTVLDGFPVAAAKTG
MLFSAPIIKAIAPILRKRDFPLVVDPVSISQSGSVLLQEDAVKALVQEILPGCDLLTPNRPEAEMLTNMTIDNLDDAAAA
GEKLLQMGARAVLIKGGHMESNVVVTDCLCMEGQAPKHLPQAKVETVNNHGTGCTLSAAIATGLGKGLPLQVAVTRAQEF
LNLALRRSYSPGNGCGPVNHSAGLNLQGI
>Mature_269_residues
MQTPPNILTIAGSDSGGGAGIQADLKTIMALGGYGMSVIAALTAQNGQGVTGIHAPDPDFVALQLCTVLDGFPVAAAKTG
MLFSAPIIKAIAPILRKRDFPLVVDPVSISQSGSVLLQEDAVKALVQEILPGCDLLTPNRPEAEMLTNMTIDNLDDAAAA
GEKLLQMGARAVLIKGGHMESNVVVTDCLCMEGQAPKHLPQAKVETVNNHGTGCTLSAAIATGLGKGLPLQVAVTRAQEF
LNLALRRSYSPGNGCGPVNHSAGLNLQGI

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=41.3127413127413, Blast_Score=170, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325378, Length=286, Percent_Identity=32.5174825174825, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324517, Length=282, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324997, Length=284, Percent_Identity=33.8028169014084, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27634; Mature: 27634

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPPNILTIAGSDSGGGAGIQADLKTIMALGGYGMSVIAALTAQNGQGVTGIHAPDPDF
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCCCH
VALQLCTVLDGFPVAAAKTGMLFSAPIIKAIAPILRKRDFPLVVDPVSISQSGSVLLQED
HHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEHHH
AVKALVQEILPGCDLLTPNRPEAEMLTNMTIDNLDDAAAAGEKLLQMGARAVLIKGGHME
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
SNVVVTDCLCMEGQAPKHLPQAKVETVNNHGTGCTLSAAIATGLGKGLPLQVAVTRAQEF
CCEEEEEEEECCCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHCCCCCCEEHHHHHHHHH
LNLALRRSYSPGNGCGPVNHSAGLNLQGI
HHHHHHHCCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MQTPPNILTIAGSDSGGGAGIQADLKTIMALGGYGMSVIAALTAQNGQGVTGIHAPDPDF
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCCCH
VALQLCTVLDGFPVAAAKTGMLFSAPIIKAIAPILRKRDFPLVVDPVSISQSGSVLLQED
HHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEHHH
AVKALVQEILPGCDLLTPNRPEAEMLTNMTIDNLDDAAAAGEKLLQMGARAVLIKGGHME
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
SNVVVTDCLCMEGQAPKHLPQAKVETVNNHGTGCTLSAAIATGLGKGLPLQVAVTRAQEF
CCEEEEEEEECCCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHCCCCCCEEHHHHHHHHH
LNLALRRSYSPGNGCGPVNHSAGLNLQGI
HHHHHHHCCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA