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The map label for this gene is fdhB [H]

Identifier: 220903832

GI number: 220903832

Start: 672535

End: 673194

Strand: Direct

Name: fdhB [H]

Synonym: Ddes_0556

Alternate gene names: 220903832

Gene position: 672535-673194 (Clockwise)

Preceding gene: 220903831

Following gene: 220903833

Centisome position: 23.41

GC content: 56.82

Gene sequence:

>660_bases
ATGCCGAAAACATTCTTAGTTGACACCACGCGGTGCACGGCATGTCGTGGCTGCCAGTTGGCCTGTAAGGAGTGGCATGA
TCTGCCTGCCAACCACACCAAGCAGCGCGGCACGCACCAGAATCCGCCGGATCTGAATCCCAACAACCTCAAGATTGTAC
GCTTTCACGAATATATCAATGACGAAGGCAATGTGGTCTGGAACTTCTTTCCCGACCAGTGCCGCCATTGCCTCACGCCG
CCCTGTGTGGATGTGGCAGACATGGCGGTGCCGGGAGCCATGATACAGGACAAGAAAACAGGGGCCGTACTGGCTACGGA
AAAATCGGCCAAGCTCAGCGAGGACGACGCCCAGGCCATACAGGAGGCCTGCCCCTATAATATTCCCAGGCGCGATCCCG
AAAACGGCCGCCTGACCAAGTGCGATATGTGCATCGACCGTGTCAGCGCGGGTATGCAGCCTATCTGCGTCAAGACCTGC
CCGACAGGGGCCATGGTTTTCGGCGAGCGTGAAGAGGTGCTGCCCCTGGCCCAGAAACGTCTTGAAAACGCCAAAAAGCG
TTGGCCCAAGGCTTTTCTGGCCGATATGGAAGATGTCAGCGTGATCTACCTGCTGGCCGACACCAAGGATAAGTATTACG
AGTTCGCGGCCTTTATGTAG

Upstream 100 bases:

>100_bases
GCCTGCTGCGTAAACCTGCGCAAGGCTGATAAACTCACCGAAATAGGCTAACGAGAAGGGCGCGCCCTTACGGTAGGGCG
CGCCTGACAAGGAGCATACT

Downstream 100 bases:

>100_bases
AGTGCTTCAGTCAGGTTTCAGTTTGGCATTGCCATAATTGTCAACTGAAATTATGCTGATGCACAGCGTTGCCGTCAGGC
CTGTACCGTATTCAACGGCT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: FDH subunit beta; Formate dehydrogenase small subunit [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MPKTFLVDTTRCTACRGCQLACKEWHDLPANHTKQRGTHQNPPDLNPNNLKIVRFHEYINDEGNVVWNFFPDQCRHCLTP
PCVDVADMAVPGAMIQDKKTGAVLATEKSAKLSEDDAQAIQEACPYNIPRRDPENGRLTKCDMCIDRVSAGMQPICVKTC
PTGAMVFGEREEVLPLAQKRLENAKKRWPKAFLADMEDVSVIYLLADTKDKYYEFAAFM

Sequences:

>Translated_219_residues
MPKTFLVDTTRCTACRGCQLACKEWHDLPANHTKQRGTHQNPPDLNPNNLKIVRFHEYINDEGNVVWNFFPDQCRHCLTP
PCVDVADMAVPGAMIQDKKTGAVLATEKSAKLSEDDAQAIQEACPYNIPRRDPENGRLTKCDMCIDRVSAGMQPICVKTC
PTGAMVFGEREEVLPLAQKRLENAKKRWPKAFLADMEDVSVIYLLADTKDKYYEFAAFM
>Mature_218_residues
PKTFLVDTTRCTACRGCQLACKEWHDLPANHTKQRGTHQNPPDLNPNNLKIVRFHEYINDEGNVVWNFFPDQCRHCLTPP
CVDVADMAVPGAMIQDKKTGAVLATEKSAKLSEDDAQAIQEACPYNIPRRDPENGRLTKCDMCIDRVSAGMQPICVKTCP
TGAMVFGEREEVLPLAQKRLENAKKRWPKAFLADMEDVSVIYLLADTKDKYYEFAAFM

Specific function: Beta chain of the formate dehydrogenase (FDH) catalyze the reversible two-electron oxidation of formate to carbon dioxide. FDH loses activity in the presence of air, but this activity can be restored. This chain is an electron transfer unit [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=214, Percent_Identity=36.4485981308411, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790326, Length=214, Percent_Identity=35.5140186915888, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787872, Length=169, Percent_Identity=33.1360946745562, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787122, Length=169, Percent_Identity=31.9526627218935, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=30.6930693069307, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI226510944, Length=179, Percent_Identity=27.9329608938547, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367345, Length=166, Percent_Identity=29.5180722891566, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 24725; Mature: 24593

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTFLVDTTRCTACRGCQLACKEWHDLPANHTKQRGTHQNPPDLNPNNLKIVRFHEYIN
CCCCEEEECCCCCHHCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEEEEEHHHHC
DEGNVVWNFFPDQCRHCLTPPCVDVADMAVPGAMIQDKKTGAVLATEKSAKLSEDDAQAI
CCCCEEEEECHHHHHHHCCCCCCCHHHHCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHH
QEACPYNIPRRDPENGRLTKCDMCIDRVSAGMQPICVKTCPTGAMVFGEREEVLPLAQKR
HHHCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHHHHHH
LENAKKRWPKAFLADMEDVSVIYLLADTKDKYYEFAAFM
HHHHHHHCHHHHHCCCCCCEEEEEEECCCHHHHHHHHCC
>Mature Secondary Structure 
PKTFLVDTTRCTACRGCQLACKEWHDLPANHTKQRGTHQNPPDLNPNNLKIVRFHEYIN
CCCEEEECCCCCHHCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEEEEEHHHHC
DEGNVVWNFFPDQCRHCLTPPCVDVADMAVPGAMIQDKKTGAVLATEKSAKLSEDDAQAI
CCCCEEEEECHHHHHHHCCCCCCCHHHHCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHH
QEACPYNIPRRDPENGRLTKCDMCIDRVSAGMQPICVKTCPTGAMVFGEREEVLPLAQKR
HHHCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHHHHHH
LENAKKRWPKAFLADMEDVSVIYLLADTKDKYYEFAAFM
HHHHHHHCHHHHHCCCCCCEEEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA