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The map label for this gene is pheS

Identifier: 220903764

GI number: 220903764

Start: 595642

End: 596679

Strand: Reverse

Name: pheS

Synonym: Ddes_0486

Alternate gene names: 220903764

Gene position: 596679-595642 (Counterclockwise)

Preceding gene: 220903765

Following gene: 220903763

Centisome position: 20.77

GC content: 61.56

Gene sequence:

>1038_bases
ATGGATCTGATTTCTGCACTGGAAGGCCTGGTCCCTGAGCTTGAAAAAGGTCTGGATCAGGCTTCTTCTTTGGAAGGGCT
TGAAGCCCTGCGGGTGGATTTTCTGGGCCGCAAGGGCAGGATTGCCCAGATCATGAGCCGCCTTCCCTCGCTCGCCCCGG
AGCAGCGCCCCGCCGTGGGGCAAACAGCCAACAGCGTCAAGGAACGCTGCAATGCGCTGTTTGAATCCCGCAAGGGCGCG
CTGAAGGCCGGGCGTGAAGCCGAAGCCCTCAAGCGCTTCGACCCCTCCGTACCCGGGCGCGCACCGTGGAGCGGCACCCT
GCACCCCACCACTCTGGTGATGGAGGAAATCTGCGGCGTTTTTCAGGGACTCGGCTTTGACGTCGCCTCCGGCCCCGAAG
TGGAGCTGGACTACTATAACTTTGAGGCCCTCAACATGCCGCCCGAGCACCCTGCCCGCGACATGCAGGATACGCTCTAT
ATCACCGACAAGGTGCTCATGCGCACCCACACCTCGCCCGTGCAGGCCCGCACCATGCTGCACCGCAAACCCCCGCTTGC
CGTTGTGGTTCCCGGCAAGGTCTACCGGCGCGACAGCGACCTTACCCATACTCCCATGTTTCATCAGATCGAAGGGCTGA
TGGTTGGTCAGGGCATCAATATGGCCCACCTGCGCGGCACACTCACGGCCTTTGTCCGCGCCATTTTCGGCAGCGACACC
CGTGTGCGCTTCCGGCCGAGCTTTTTTCCCTTTACCGAGCCTTCTGCCGAGGTGGACATGAGCTGCTGCATGTGCGGCGG
CCAGGGCCATATTGACGGCAGCCCCTGCCGTGTGTGCAAAACCACGGGCTGGGTTGAGATTCTCGGCTGCGGCATGGTGG
ACCCGGCAGTGTTTGAAGCCGTTGGTTATGATGCCGATGTCAGCGGCTTTGCCTTCGGTATGGGCGTGGAGCGCGTGACC
ATGCTCAAATACGGCATTGGCGACCTGCGCATGTTCTTTGAAAACGACGTACGCTTTCTGGGCCAATTCGCCCGCTGA

Upstream 100 bases:

>100_bases
GCTATAAGGGCGTGGGCGGTGCACATGTGCTGCCCACGCCCTGTTTTTGAGCCACACAAAATTCAACGTCAGCGCCGCGC
AGGCGGCATCGGGAATCCGT

Downstream 100 bases:

>100_bases
GGCCAAAGCCGTGTTGCGTTCCGCCAGCCGTCCCTGCCGTCCTCGTGCCGCCTCCGGCATGCTGCGTCACGCCCTTGCCT
GCGCAGTGACGGCAGCCCTG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDLISALEGLVPELEKGLDQASSLEGLEALRVDFLGRKGRIAQIMSRLPSLAPEQRPAVGQTANSVKERCNALFESRKGA
LKAGREAEALKRFDPSVPGRAPWSGTLHPTTLVMEEICGVFQGLGFDVASGPEVELDYYNFEALNMPPEHPARDMQDTLY
ITDKVLMRTHTSPVQARTMLHRKPPLAVVVPGKVYRRDSDLTHTPMFHQIEGLMVGQGINMAHLRGTLTAFVRAIFGSDT
RVRFRPSFFPFTEPSAEVDMSCCMCGGQGHIDGSPCRVCKTTGWVEILGCGMVDPAVFEAVGYDADVSGFAFGMGVERVT
MLKYGIGDLRMFFENDVRFLGQFAR

Sequences:

>Translated_345_residues
MDLISALEGLVPELEKGLDQASSLEGLEALRVDFLGRKGRIAQIMSRLPSLAPEQRPAVGQTANSVKERCNALFESRKGA
LKAGREAEALKRFDPSVPGRAPWSGTLHPTTLVMEEICGVFQGLGFDVASGPEVELDYYNFEALNMPPEHPARDMQDTLY
ITDKVLMRTHTSPVQARTMLHRKPPLAVVVPGKVYRRDSDLTHTPMFHQIEGLMVGQGINMAHLRGTLTAFVRAIFGSDT
RVRFRPSFFPFTEPSAEVDMSCCMCGGQGHIDGSPCRVCKTTGWVEILGCGMVDPAVFEAVGYDADVSGFAFGMGVERVT
MLKYGIGDLRMFFENDVRFLGQFAR
>Mature_345_residues
MDLISALEGLVPELEKGLDQASSLEGLEALRVDFLGRKGRIAQIMSRLPSLAPEQRPAVGQTANSVKERCNALFESRKGA
LKAGREAEALKRFDPSVPGRAPWSGTLHPTTLVMEEICGVFQGLGFDVASGPEVELDYYNFEALNMPPEHPARDMQDTLY
ITDKVLMRTHTSPVQARTMLHRKPPLAVVVPGKVYRRDSDLTHTPMFHQIEGLMVGQGINMAHLRGTLTAFVRAIFGSDT
RVRFRPSFFPFTEPSAEVDMSCCMCGGQGHIDGSPCRVCKTTGWVEILGCGMVDPAVFEAVGYDADVSGFAFGMGVERVT
MLKYGIGDLRMFFENDVRFLGQFAR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=278, Percent_Identity=30.5755395683453, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI5729820, Length=290, Percent_Identity=30.6896551724138, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788007, Length=343, Percent_Identity=47.8134110787172, Blast_Score=322, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32563657, Length=289, Percent_Identity=30.7958477508651, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17508957, Length=289, Percent_Identity=30.7958477508651, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566635, Length=257, Percent_Identity=33.4630350194552, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=275, Percent_Identity=30.9090909090909, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI18858079, Length=292, Percent_Identity=29.4520547945205, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17137424, Length=297, Percent_Identity=25.5892255892256, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_DESDA (B8J4E0)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479076.1
- GeneID:   7284151
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0486
- HOGENOM:   HBG284353
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37967; Mature: 37967

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLISALEGLVPELEKGLDQASSLEGLEALRVDFLGRKGRIAQIMSRLPSLAPEQRPAVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
QTANSVKERCNALFESRKGALKAGREAEALKRFDPSVPGRAPWSGTLHPTTLVMEEICGV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FQGLGFDVASGPEVELDYYNFEALNMPPEHPARDMQDTLYITDKVLMRTHTSPVQARTML
HHCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
HRKPPLAVVVPGKVYRRDSDLTHTPMFHQIEGLMVGQGINMAHLRGTLTAFVRAIFGSDT
HCCCCEEEEECCHHEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
RVRFRPSFFPFTEPSAEVDMSCCMCGGQGHIDGSPCRVCKTTGWVEILGCGMVDPAVFEA
EEEECCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCEEEECCCCEEEEECCCCCHHHHHH
VGYDADVSGFAFGMGVERVTMLKYGIGDLRMFFENDVRFLGQFAR
HCCCCCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLISALEGLVPELEKGLDQASSLEGLEALRVDFLGRKGRIAQIMSRLPSLAPEQRPAVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
QTANSVKERCNALFESRKGALKAGREAEALKRFDPSVPGRAPWSGTLHPTTLVMEEICGV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FQGLGFDVASGPEVELDYYNFEALNMPPEHPARDMQDTLYITDKVLMRTHTSPVQARTML
HHCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
HRKPPLAVVVPGKVYRRDSDLTHTPMFHQIEGLMVGQGINMAHLRGTLTAFVRAIFGSDT
HCCCCEEEEECCHHEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
RVRFRPSFFPFTEPSAEVDMSCCMCGGQGHIDGSPCRVCKTTGWVEILGCGMVDPAVFEA
EEEECCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCEEEECCCCEEEEECCCCCHHHHHH
VGYDADVSGFAFGMGVERVTMLKYGIGDLRMFFENDVRFLGQFAR
HCCCCCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA