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The map label for this gene is wcaL [C]

Identifier: 220903748

GI number: 220903748

Start: 575933

End: 577303

Strand: Reverse

Name: wcaL [C]

Synonym: Ddes_0470

Alternate gene names: 220903748

Gene position: 577303-575933 (Counterclockwise)

Preceding gene: 220903749

Following gene: 220903747

Centisome position: 20.09

GC content: 61.85

Gene sequence:

>1371_bases
ATGCGAGTTCTTATGTTCGGCTGGGAATTTCCTCCCCATATCAGCGGCGGCCTTGGCACCGCCTGCTTTGGCATGACCCA
GGCCCTGGCCCGTAAAGGGGCGGAAATCATCTTTGTACTGCCCCGTATCGACAGTGACGTGGAACAAGAGGGCTTTCTGC
GTTTACGCCCGGCTTCGGGCACACCCATTACCGAAGATCTGGCCCGGCGCATGGAGCCTACGCAGTATGAGCACAAGGTG
GTATGGCCCGGCGGCGTTCGCTGCATGCCAGTAGACAGCCCCCTCATGCCCTACCTGACGCCGCACGAGTATGTGCGTGC
CCTGGGCCGCCACGATGTGCATGAAAGCGCCGGGCCTGCCGGCCTTTCCGTTCCGCCCGCGCCTGCCGCCTCAAAAATGC
GCGGCCCACGCCGAAAAACCTTTACCTATACCCTGCACGGCGGCTACGGGCCTGACCTCATGCGCGAAGTGCTGCGCTAC
GGCCGCCTGGCCGCAGCCCTGGCCGCCCGTGAAAATTTTGACGTTATCCACGCCCACGACTGGATGACCTACCCCGCGGG
CATGCTGGCAAAAAACATCACGGGCAAGCCCCTTGTGGTTCACATCCATGCCACGGAATACGACCGCAGCGGAGACAGTA
TCAATGAACAGGTGGCGGGCGTTGAGCGCGCAGGCATGCTGGCCGCCGATGTTGTGGTAGCCGTGAGCCGCCTCACCCGC
AAGACCGTTATAGAGCGTTACGGCATCCCCCCGGAAAAGGTGCTGGTGGTGCACAATGCCGTAGCCCGGCACGAGGCGCG
GCGGCGCTATCCTGTTCCCCCGCGCATCCGGCATGAAAAACGCGTGCTCTTTCTGGGCCGTGTAACCTTTCAGAAAGGAC
CGGAATACTTCATGGAAGCAGCGCGCCTTGTGCTGCAAAAGATTCCTGAGGCCCGCTTTTTCATGGCGGGCAGCGGCGAC
ATGCTGCCCCGGCTCATCCGCCGGGCGGGCCAGTTGCGCATGGGTAGCCGCTTTCATTTCGCGGGCTTTCTGCGCGGCGA
AGAAGTGGACCGCATGTTTGCCCTGAGCGACCTGTACGTCATGCCCTCGGTTTCCGAACCGTTCGGCATCACGCCGCTTG
AGGCCATGCTTTATGATGTGCCCGTGCTGCTTTCGCGCCAGTCCGGCGTATCGGAAGTACTGGAGCACGCCCTCAAGGCC
GACTTCTGGGACACACGCGACATGGCCGACAAAATCTGCGCCGTGCTGCGCTACCCCTGCCTTGCCGCCGAACTGGTGAA
AAACTGCCACGAAGAAATGAAGTCCATCCGCTGGGAAAACGCCGCCGACCAGTTGCTGGTCATTTACCGCAACGTACAGG
GAGGTCACTGA

Upstream 100 bases:

>100_bases
GACCGCAAGTATTGACCACAGTGGAGGAAGAAACACATTTGAGGCAGCCCACAACAGCGGCTGCCGAACCTGGCGGCTGT
GCCGCCACGAGGGAGAATCC

Downstream 100 bases:

>100_bases
TGCCAGCCCTCTGCTTCTGCTATGAGGTTCACGAACCTTACCAGCTGCGCCGCTATACCGTCTTTGACATGGGTCAGAAC
TCCATCTACGAGGAAGACGA

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRVLMFGWEFPPHISGGLGTACFGMTQALARKGAEIIFVLPRIDSDVEQEGFLRLRPASGTPITEDLARRMEPTQYEHKV
VWPGGVRCMPVDSPLMPYLTPHEYVRALGRHDVHESAGPAGLSVPPAPAASKMRGPRRKTFTYTLHGGYGPDLMREVLRY
GRLAAALAARENFDVIHAHDWMTYPAGMLAKNITGKPLVVHIHATEYDRSGDSINEQVAGVERAGMLAADVVVAVSRLTR
KTVIERYGIPPEKVLVVHNAVARHEARRRYPVPPRIRHEKRVLFLGRVTFQKGPEYFMEAARLVLQKIPEARFFMAGSGD
MLPRLIRRAGQLRMGSRFHFAGFLRGEEVDRMFALSDLYVMPSVSEPFGITPLEAMLYDVPVLLSRQSGVSEVLEHALKA
DFWDTRDMADKICAVLRYPCLAAELVKNCHEEMKSIRWENAADQLLVIYRNVQGGH

Sequences:

>Translated_456_residues
MRVLMFGWEFPPHISGGLGTACFGMTQALARKGAEIIFVLPRIDSDVEQEGFLRLRPASGTPITEDLARRMEPTQYEHKV
VWPGGVRCMPVDSPLMPYLTPHEYVRALGRHDVHESAGPAGLSVPPAPAASKMRGPRRKTFTYTLHGGYGPDLMREVLRY
GRLAAALAARENFDVIHAHDWMTYPAGMLAKNITGKPLVVHIHATEYDRSGDSINEQVAGVERAGMLAADVVVAVSRLTR
KTVIERYGIPPEKVLVVHNAVARHEARRRYPVPPRIRHEKRVLFLGRVTFQKGPEYFMEAARLVLQKIPEARFFMAGSGD
MLPRLIRRAGQLRMGSRFHFAGFLRGEEVDRMFALSDLYVMPSVSEPFGITPLEAMLYDVPVLLSRQSGVSEVLEHALKA
DFWDTRDMADKICAVLRYPCLAAELVKNCHEEMKSIRWENAADQLLVIYRNVQGGH
>Mature_456_residues
MRVLMFGWEFPPHISGGLGTACFGMTQALARKGAEIIFVLPRIDSDVEQEGFLRLRPASGTPITEDLARRMEPTQYEHKV
VWPGGVRCMPVDSPLMPYLTPHEYVRALGRHDVHESAGPAGLSVPPAPAASKMRGPRRKTFTYTLHGGYGPDLMREVLRY
GRLAAALAARENFDVIHAHDWMTYPAGMLAKNITGKPLVVHIHATEYDRSGDSINEQVAGVERAGMLAADVVVAVSRLTR
KTVIERYGIPPEKVLVVHNAVARHEARRRYPVPPRIRHEKRVLFLGRVTFQKGPEYFMEAARLVLQKIPEARFFMAGSGD
MLPRLIRRAGQLRMGSRFHFAGFLRGEEVDRMFALSDLYVMPSVSEPFGITPLEAMLYDVPVLLSRQSGVSEVLEHALKA
DFWDTRDMADKICAVLRYPCLAAELVKNCHEEMKSIRWENAADQLLVIYRNVQGGH

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI9755344, Length=296, Percent_Identity=22.6351351351351, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 51185; Mature: 51185

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLMFGWEFPPHISGGLGTACFGMTQALARKGAEIIFVLPRIDSDVEQEGFLRLRPASG
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCC
TPITEDLARRMEPTQYEHKVVWPGGVRCMPVDSPLMPYLTPHEYVRALGRHDVHESAGPA
CCHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHCCCCC
GLSVPPAPAASKMRGPRRKTFTYTLHGGYGPDLMREVLRYGRLAAALAARENFDVIHAHD
CCCCCCCCCHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
WMTYPAGMLAKNITGKPLVVHIHATEYDRSGDSINEQVAGVERAGMLAADVVVAVSRLTR
CCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTVIERYGIPPEKVLVVHNAVARHEARRRYPVPPRIRHEKRVLFLGRVTFQKGPEYFMEA
HHHHHHHCCCHHHEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCHHHHHHH
ARLVLQKIPEARFFMAGSGDMLPRLIRRAGQLRMGSRFHFAGFLRGEEVDRMFALSDLYV
HHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHEE
MPSVSEPFGITPLEAMLYDVPVLLSRQSGVSEVLEHALKADFWDTRDMADKICAVLRYPC
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHH
LAAELVKNCHEEMKSIRWENAADQLLVIYRNVQGGH
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRVLMFGWEFPPHISGGLGTACFGMTQALARKGAEIIFVLPRIDSDVEQEGFLRLRPASG
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCC
TPITEDLARRMEPTQYEHKVVWPGGVRCMPVDSPLMPYLTPHEYVRALGRHDVHESAGPA
CCHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHCCCCC
GLSVPPAPAASKMRGPRRKTFTYTLHGGYGPDLMREVLRYGRLAAALAARENFDVIHAHD
CCCCCCCCCHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
WMTYPAGMLAKNITGKPLVVHIHATEYDRSGDSINEQVAGVERAGMLAADVVVAVSRLTR
CCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTVIERYGIPPEKVLVVHNAVARHEARRRYPVPPRIRHEKRVLFLGRVTFQKGPEYFMEA
HHHHHHHCCCHHHEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCHHHHHHH
ARLVLQKIPEARFFMAGSGDMLPRLIRRAGQLRMGSRFHFAGFLRGEEVDRMFALSDLYV
HHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHEE
MPSVSEPFGITPLEAMLYDVPVLLSRQSGVSEVLEHALKADFWDTRDMADKICAVLRYPC
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHH
LAAELVKNCHEEMKSIRWENAADQLLVIYRNVQGGH
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]