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The map label for this gene is lacA [C]

Identifier: 220903685

GI number: 220903685

Start: 495200

End: 495871

Strand: Reverse

Name: lacA [C]

Synonym: Ddes_0407

Alternate gene names: 220903685

Gene position: 495871-495200 (Counterclockwise)

Preceding gene: 220903686

Following gene: 220903684

Centisome position: 17.26

GC content: 46.88

Gene sequence:

>672_bases
ATGAATTTGAAAAATAAACTTATCAGTTTGTTGTTTAATACACTGAAAATGTGCAGGAATGTTTATATCTTTTTTTCAAC
ACAGTATAATACAATATTTTTTAAACTCTGGGCAATAGCCTCGCGGGTTCCGTTTGGCAGCAAATTGCAATGTTGTGGGA
GGGTTATCCTCGATTGCCTGCCCGCCACCGTCTCGCTTGGAAATAACATTATTTTTGTCTCAGATTCAGTGCGTTGCACA
GCAACAACCGTTTATGGGCCCTCACGCTTGCGGACATTCGCGCCCACTGCCAGAATTGAAATTGCCGACGGTGTCGGAAT
GAATGCCATCAGCATTACCGCCAGGAGCAGAACTATCCACATAGGTGAAAATACCATGTTTGCGCCTAACTGCACGGTTG
TTGATTCAGATTTTCATTCTCTCTGGCCGCCGGAAGGGCGGATATGTAATCCGGGGATGGAACAGGACGCCGATGTGCAT
ATAGGGAAAAACTGCTGGATCGGCATGCAATCCATTATTTTAAAAGGCGTAACAATTGGAGATGGCTCGGTAGTTGCCGC
TGGTTCGGTGGTGACTCGCGACATTCCCCCCAATGTATTGGCCGCTGGCGTTCCCGCCAGGGTGCTCCGTCCTCTTAATG
ATAACGAGGGTAATACATGCGTATTGGTGTAG

Upstream 100 bases:

>100_bases
GCAGCCTTTCTCAGGATGTATTATAATACCATTGTATATTTGGATATGCTCAGAATTTGAATAGTTAAATTTTAAGCACG
AAATAGCTATGAGGCAACAT

Downstream 100 bases:

>100_bases
CCGCAGAACTTCAACATGCAAATGCCGCAGATGTTTTGCTGTGGGACAGCTTCAGCGATACGGTTCCATGCGTCGAAAAA
CTCGTTCAGGAGTATTTTCC

Product: transferase hexapeptide repeat containing protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MNLKNKLISLLFNTLKMCRNVYIFFSTQYNTIFFKLWAIASRVPFGSKLQCCGRVILDCLPATVSLGNNIIFVSDSVRCT
ATTVYGPSRLRTFAPTARIEIADGVGMNAISITARSRTIHIGENTMFAPNCTVVDSDFHSLWPPEGRICNPGMEQDADVH
IGKNCWIGMQSIILKGVTIGDGSVVAAGSVVTRDIPPNVLAAGVPARVLRPLNDNEGNTCVLV

Sequences:

>Translated_223_residues
MNLKNKLISLLFNTLKMCRNVYIFFSTQYNTIFFKLWAIASRVPFGSKLQCCGRVILDCLPATVSLGNNIIFVSDSVRCT
ATTVYGPSRLRTFAPTARIEIADGVGMNAISITARSRTIHIGENTMFAPNCTVVDSDFHSLWPPEGRICNPGMEQDADVH
IGKNCWIGMQSIILKGVTIGDGSVVAAGSVVTRDIPPNVLAAGVPARVLRPLNDNEGNTCVLV
>Mature_223_residues
MNLKNKLISLLFNTLKMCRNVYIFFSTQYNTIFFKLWAIASRVPFGSKLQCCGRVILDCLPATVSLGNNIIFVSDSVRCT
ATTVYGPSRLRTFAPTARIEIADGVGMNAISITARSRTIHIGENTMFAPNCTVVDSDFHSLWPPEGRICNPGMEQDADVH
IGKNCWIGMQSIILKGVTIGDGSVVAAGSVVTRDIPPNVLAAGVPARVLRPLNDNEGNTCVLV

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=99, Percent_Identity=46.4646464646465, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786664, Length=94, Percent_Identity=43.6170212765958, Blast_Score=74, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322243, Length=123, Percent_Identity=37.3983739837398, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 24192; Mature: 24192

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKNKLISLLFNTLKMCRNVYIFFSTQYNTIFFKLWAIASRVPFGSKLQCCGRVILDCL
CCHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEHEEEHHHHCCCCCCHHHHHHHHHHHHH
PATVSLGNNIIFVSDSVRCTATTVYGPSRLRTFAPTARIEIADGVGMNAISITARSRTIH
HHHHHCCCEEEEEECCCEEEEEEEECHHHHHCCCCCEEEEEECCCCCCEEEEEEECEEEE
IGENTMFAPNCTVVDSDFHSLWPPEGRICNPGMEQDADVHIGKNCWIGMQSIILKGVTIG
ECCCEEECCCCEEEECCHHHCCCCCCCEECCCCCCCCCEEECCCCEECHHHHHEEEEEEC
DGSVVAAGSVVTRDIPPNVLAAGVPARVLRPLNDNEGNTCVLV
CCCEEEECCEEECCCCCHHHCCCCCHHHHCCCCCCCCCEEEEC
>Mature Secondary Structure
MNLKNKLISLLFNTLKMCRNVYIFFSTQYNTIFFKLWAIASRVPFGSKLQCCGRVILDCL
CCHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEHEEEHHHHCCCCCCHHHHHHHHHHHHH
PATVSLGNNIIFVSDSVRCTATTVYGPSRLRTFAPTARIEIADGVGMNAISITARSRTIH
HHHHHCCCEEEEEECCCEEEEEEEECHHHHHCCCCCEEEEEECCCCCCEEEEEEECEEEE
IGENTMFAPNCTVVDSDFHSLWPPEGRICNPGMEQDADVHIGKNCWIGMQSIILKGVTIG
ECCCEEECCCCEEEECCHHHCCCCCCCEECCCCCCCCCEEECCCCEECHHHHHEEEEEEC
DGSVVAAGSVVTRDIPPNVLAAGVPARVLRPLNDNEGNTCVLV
CCCEEEECCEEECCCCCHHHCCCCCHHHHCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]