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The map label for this gene is mltD [H]

Identifier: 220903659

GI number: 220903659

Start: 456912

End: 458579

Strand: Direct

Name: mltD [H]

Synonym: Ddes_0380

Alternate gene names: 220903659

Gene position: 456912-458579 (Clockwise)

Preceding gene: 220903657

Following gene: 220903664

Centisome position: 15.9

GC content: 61.09

Gene sequence:

>1668_bases
ATGACGGAACATACTATGGGCACTGTCGCGCAAGGACGCAAGGGGGCAGGTGCCGGTATTCGCCTTTGTGTGCTGGCTGC
CCTGTGCTGCCTGCTGCTGGCCGGAGGCTGCGCCTCGCGCCAGGGCGGCTCGGGTGGCGGCGGTGCTTCTTTTTCCGTCC
CTGATGACGATCCCACGCCCTTGAGTCCCAAAGAGGTCGCTGCCCTCAATTCTACCGGGCATGTTGATAAAAGCCTGCCC
CCGGAAGCTATGGACGATGTGGCACGTCAATATAAGTATTTTTTGCGCAAGGGACGCAATTCCGTATGTGTGTTCTCGAA
ACGCGCGGAAAACTACCTGGCCTATGCCCGCAAGGTGTTTCGCTCGCGCGGCATGCCCGAAGAACTGGCTTACCTTGCCA
TTGTGGAAAGCGGGTACAGGGTGGACGCCCGTTCGCCTGTGGGCGCTACCGGAGCCTGGCAGTTCATGCCGCCTACGGGT
GAGCGCTTCGGGTTGACGCAGAACTGGTTTACTGACGAACGCCTTGACCCGTACGAATCCACGGAGGCTGCGGCGGACTA
TCTGCAGAAGCTGCACGAGTATTTCGGCGACTGGCCCACGGCCATCGCGGCCTATAACGCGGGTGAAGGCAAGATCGGCC
GTGCCAAGGCGGGTACAGGCGGCAAGGATTTTTTTGAGGTCAAGGCCCGCAATCACATGCTGGATGAAAAAGCGCAACTG
CGCGATGAGACCAAGCAGTATGTGCCGCGCTTTCTGGCAGTTACAAAAATCATGCGTAATCTGCCCGAACTGGGTTTCGA
GCCGATCAGTCCCGAAAGCGCGCCCGGCGTGCTGCGCCTTACGGCGCGCCCCGGAACCGATCTCAACGGCCTGGCTGATG
CCTGCGGCATGAGCTGGGGCGATTTCATTTCCTATAACCGTCATCATAAACGCCGTATTACTGATACGGACCGTTCCACC
TTTGTCTATGTTCCCGCCAGAATAGAGCGCCAGGCCACGGCCTTTCTTTCTTCGCCCCGTTGCACCGCCTATGCGGACTG
GGGCGCGGTGCGCGTGGCCAGCAATGCGGATTCGTGGGACAAGATCAGCCGCCGTTGCGGTATCCCTGCGACGCAGTTGC
GCGCCCTTAATCCCGGTGAAAGCCGCCTGCGCGCCGGGCAGACCGTGCTTGCGCCGCGCTCGGTGGACATGTCCTCCAGG
GCTGTGGCCGCGCTGGACAACAAGGTTCAGGCCCAGTCGGGCAACCGGGGGCGGGCTTCGGCACAGCCAGCAGGACGGCA
GACAGCGGCGCAGGGCGGCAGTCATACGCTTCAGGCTGGTGAAACCCTGTATGCCGTGGCCCGTCAGTATAACATCAGCG
TGAAGGACCTTCAGGACCATAATAATATAAGCAGTCCCAATATGATTCATGCCGGAACCGTGCTGCGCATCCCCGCCCGC
GCCGTGGCCCAGGCCGGGCCTGTTTCCGGCGGATCTGACGGCAGGGTGGGGCGCAAGGCCGGTAGCGGAGCGGCAAAGGC
CGCCAAAAGCACCTATCTTGTGCAGGCCAGAGACAGCCTGTGGAAGATCGCCCGTGAACATAATGTAAGTGTTGAAGACC
TCAAGCGTTGGAACGGTGTGGATGAAAAGACCCTGCGGGCCGGATCCGTGCTGGTGGTGGAGCAGTAG

Upstream 100 bases:

>100_bases
CTGCCGGGCTAAAAATATAGATACAACGGGCGTTCACGGCCATGCGGCTGTGTACGCACCTTCCCGGCGCATGGCAAGCC
GGGCGGACAAGGAGTGCATT

Downstream 100 bases:

>100_bases
CAAGCCTGTTTGCGGCAGCCGCCCGAACGCGACAGCAGGTTTTTTGCTGCGGGCCAGTCGATGCGAGAGCGTTGCAAGCT
GTTTATGTGATTGAAACTGT

Product: lytic transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTEHTMGTVAQGRKGAGAGIRLCVLAALCCLLLAGGCASRQGGSGGGGASFSVPDDDPTPLSPKEVAALNSTGHVDKSLP
PEAMDDVARQYKYFLRKGRNSVCVFSKRAENYLAYARKVFRSRGMPEELAYLAIVESGYRVDARSPVGATGAWQFMPPTG
ERFGLTQNWFTDERLDPYESTEAAADYLQKLHEYFGDWPTAIAAYNAGEGKIGRAKAGTGGKDFFEVKARNHMLDEKAQL
RDETKQYVPRFLAVTKIMRNLPELGFEPISPESAPGVLRLTARPGTDLNGLADACGMSWGDFISYNRHHKRRITDTDRST
FVYVPARIERQATAFLSSPRCTAYADWGAVRVASNADSWDKISRRCGIPATQLRALNPGESRLRAGQTVLAPRSVDMSSR
AVAALDNKVQAQSGNRGRASAQPAGRQTAAQGGSHTLQAGETLYAVARQYNISVKDLQDHNNISSPNMIHAGTVLRIPAR
AVAQAGPVSGGSDGRVGRKAGSGAAKAAKSTYLVQARDSLWKIAREHNVSVEDLKRWNGVDEKTLRAGSVLVVEQ

Sequences:

>Translated_555_residues
MTEHTMGTVAQGRKGAGAGIRLCVLAALCCLLLAGGCASRQGGSGGGGASFSVPDDDPTPLSPKEVAALNSTGHVDKSLP
PEAMDDVARQYKYFLRKGRNSVCVFSKRAENYLAYARKVFRSRGMPEELAYLAIVESGYRVDARSPVGATGAWQFMPPTG
ERFGLTQNWFTDERLDPYESTEAAADYLQKLHEYFGDWPTAIAAYNAGEGKIGRAKAGTGGKDFFEVKARNHMLDEKAQL
RDETKQYVPRFLAVTKIMRNLPELGFEPISPESAPGVLRLTARPGTDLNGLADACGMSWGDFISYNRHHKRRITDTDRST
FVYVPARIERQATAFLSSPRCTAYADWGAVRVASNADSWDKISRRCGIPATQLRALNPGESRLRAGQTVLAPRSVDMSSR
AVAALDNKVQAQSGNRGRASAQPAGRQTAAQGGSHTLQAGETLYAVARQYNISVKDLQDHNNISSPNMIHAGTVLRIPAR
AVAQAGPVSGGSDGRVGRKAGSGAAKAAKSTYLVQARDSLWKIAREHNVSVEDLKRWNGVDEKTLRAGSVLVVEQ
>Mature_554_residues
TEHTMGTVAQGRKGAGAGIRLCVLAALCCLLLAGGCASRQGGSGGGGASFSVPDDDPTPLSPKEVAALNSTGHVDKSLPP
EAMDDVARQYKYFLRKGRNSVCVFSKRAENYLAYARKVFRSRGMPEELAYLAIVESGYRVDARSPVGATGAWQFMPPTGE
RFGLTQNWFTDERLDPYESTEAAADYLQKLHEYFGDWPTAIAAYNAGEGKIGRAKAGTGGKDFFEVKARNHMLDEKAQLR
DETKQYVPRFLAVTKIMRNLPELGFEPISPESAPGVLRLTARPGTDLNGLADACGMSWGDFISYNRHHKRRITDTDRSTF
VYVPARIERQATAFLSSPRCTAYADWGAVRVASNADSWDKISRRCGIPATQLRALNPGESRLRAGQTVLAPRSVDMSSRA
VAALDNKVQAQSGNRGRASAQPAGRQTAAQGGSHTLQAGETLYAVARQYNISVKDLQDHNNISSPNMIHAGTVLRIPARA
VAQAGPVSGGSDGRVGRKAGSGAAKAAKSTYLVQARDSLWKIAREHNVSVEDLKRWNGVDEKTLRAGSVLVVEQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=320, Percent_Identity=29.375, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 59921; Mature: 59790

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTMGTVAQGRKGAGAGIRLCVLAALCCLLLAGGCASRQGGSGGGGASFSVPDDDPTP
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LSPKEVAALNSTGHVDKSLPPEAMDDVARQYKYFLRKGRNSVCVFSKRAENYLAYARKVF
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
RSRGMPEELAYLAIVESGYRVDARSPVGATGAWQFMPPTGERFGLTQNWFTDERLDPYES
HHCCCHHHHHHHEEECCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHH
TEAAADYLQKLHEYFGDWPTAIAAYNAGEGKIGRAKAGTGGKDFFEVKARNHMLDEKAQL
HHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHH
RDETKQYVPRFLAVTKIMRNLPELGFEPISPESAPGVLRLTARPGTDLNGLADACGMSWG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHH
DFISYNRHHKRRITDTDRSTFVYVPARIERQATAFLSSPRCTAYADWGAVRVASNADSWD
HHHHCCCHHHCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHH
KISRRCGIPATQLRALNPGESRLRAGQTVLAPRSVDMSSRAVAALDNKVQAQSGNRGRAS
HHHHHCCCCHHHHEECCCCHHHHHCCCEEECCCCCCCCHHHHHHHHCHHHCCCCCCCCCC
AQPAGRQTAAQGGSHTLQAGETLYAVARQYNISVKDLQDHNNISSPNMIHAGTVLRIPAR
CCCCCCHHHHCCCCCCHHCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEECCCEEECCHH
AVAQAGPVSGGSDGRVGRKAGSGAAKAAKSTYLVQARDSLWKIAREHNVSVEDLKRWNGV
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEHHHHHHHHHHHCCCCHHHHHHHCCC
DEKTLRAGSVLVVEQ
CHHHHCCCCEEEEEC
>Mature Secondary Structure 
TEHTMGTVAQGRKGAGAGIRLCVLAALCCLLLAGGCASRQGGSGGGGASFSVPDDDPTP
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LSPKEVAALNSTGHVDKSLPPEAMDDVARQYKYFLRKGRNSVCVFSKRAENYLAYARKVF
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
RSRGMPEELAYLAIVESGYRVDARSPVGATGAWQFMPPTGERFGLTQNWFTDERLDPYES
HHCCCHHHHHHHEEECCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHH
TEAAADYLQKLHEYFGDWPTAIAAYNAGEGKIGRAKAGTGGKDFFEVKARNHMLDEKAQL
HHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHH
RDETKQYVPRFLAVTKIMRNLPELGFEPISPESAPGVLRLTARPGTDLNGLADACGMSWG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHH
DFISYNRHHKRRITDTDRSTFVYVPARIERQATAFLSSPRCTAYADWGAVRVASNADSWD
HHHHCCCHHHCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHH
KISRRCGIPATQLRALNPGESRLRAGQTVLAPRSVDMSSRAVAALDNKVQAQSGNRGRAS
HHHHHCCCCHHHHEECCCCHHHHHCCCEEECCCCCCCCHHHHHHHHCHHHCCCCCCCCCC
AQPAGRQTAAQGGSHTLQAGETLYAVARQYNISVKDLQDHNNISSPNMIHAGTVLRIPAR
CCCCCCHHHHCCCCCCHHCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEECCCEEECCHH
AVAQAGPVSGGSDGRVGRKAGSGAAKAAKSTYLVQARDSLWKIAREHNVSVEDLKRWNGV
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEHHHHHHHHHHHCCCCHHHHHHHCCC
DEKTLRAGSVLVVEQ
CHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]