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The map label for this gene is acrA [H]

Identifier: 220903588

GI number: 220903588

Start: 374918

End: 376033

Strand: Direct

Name: acrA [H]

Synonym: Ddes_0307

Alternate gene names: 220903588

Gene position: 374918-376033 (Clockwise)

Preceding gene: 220903587

Following gene: 220903589

Centisome position: 13.05

GC content: 58.6

Gene sequence:

>1116_bases
ATGACAAGCAGACAGCTGTTCTCTCTTCCTCTTGCCCTGGCGCTTTGCCTCGCGCTGGTCGCCTGCGATAGTGAAAAAAA
AGCCCAGCAGGGGGCCATGCACCTGCCCGTGGCAATTTTTGATGTTGTAGCTCGCGATGAACCGTGGCCTGCCGAATATC
AGGCGCAGGCATCCGGCTCGCGGGCTGTTGAAGTGCGCGCGCGGGTTGAAGCCATTATTGAAAAGCGGCTGTATGAAGAA
GGCGATTATGTGGAGCAGGGCCAACTGCTTTTCCAGTTGGAACGCGACCATTACGAAGCGCTCATGCAGCAGGCTCAGGC
CCAGTTCACCAATGCCGAACGGGAATGGCGGCGGGTGCGCCCGCTGTATGAAAAGAATGCCGTGTCGCAGAAAGAGCGCG
ACAATGCCCTGGCCGCCTATGAAAGCGCCAAGGCAGCCATGCGCCAGGCCAAGATCAATCTGGATTACTGTCAGGTGACG
GCCCCGGTCTCCGGCTACAGCAGCAAGGAAAACTACACCCCGGGCAACCTCGTGGGCAACAATTCCCTGCTTACCTACGT
GAACCAGACCGACCCGATGTACATCGATTTTTCCATTGCCGCTCCCGAGCGCATGACACGCCAGAACCTGGCCGCCGAAG
GCCGCCTCGCGCTGCCTGCCGAAGGCCGTTACAAGGCGCGGCTGCGGCTGCTTGACGGCAGCATGTACAAGGGCGAGGGC
GAAGTGACTTTTATTGACAGCCAGGTACAGCCAAGCACCGGCGTCATCAAGGCCCGTGCGGTTTTTGCCAATGCTGACCG
CAGCATCATGCCCGGTCAGTATGTCCGGCTGTATATGGATGGCGATATCCTTAAAAACGCCGTGCTTGTTCCGCAAAAGT
GCGTACTGCTGACCCAGAAGGGTTCGCTGGTTATGGGCGTGGACAAGGACGACAAGGTGTATCCCATTCCCGTGGTCGTT
GGTGTGTCCGTTGGCGACAAATATCTTGTGCTTGAGGGTCTCAAGGGCGGCGAACGCATTATCAGCGAAGGCATCATCAA
GGCCCGTCCCGGCACACAAGTGAGCATAATGCCCTCCGGGGGCGAACAGCCCCGGGATGGCGCGGCTAAGAAGTAG

Upstream 100 bases:

>100_bases
TTTTGCAATGAACTGTGTTTTTTACATTGATGCTTCGCCCGATTTTGTATGCCGGGCGGTTTTTTCAGCGTGCCGAGTCA
CGCGTCTTATGGAGTAGTTC

Downstream 100 bases:

>100_bases
GTAAGTATGGCTGTTTCCACAAAGCCGAATTTCTTTTTACGCAGACCAATTCTTTCGGCGGTCATATCCATTGTCATCAC
TCTGGTGGGGGCGCTGGCCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTSRQLFSLPLALALCLALVACDSEKKAQQGAMHLPVAIFDVVARDEPWPAEYQAQASGSRAVEVRARVEAIIEKRLYEE
GDYVEQGQLLFQLERDHYEALMQQAQAQFTNAEREWRRVRPLYEKNAVSQKERDNALAAYESAKAAMRQAKINLDYCQVT
APVSGYSSKENYTPGNLVGNNSLLTYVNQTDPMYIDFSIAAPERMTRQNLAAEGRLALPAEGRYKARLRLLDGSMYKGEG
EVTFIDSQVQPSTGVIKARAVFANADRSIMPGQYVRLYMDGDILKNAVLVPQKCVLLTQKGSLVMGVDKDDKVYPIPVVV
GVSVGDKYLVLEGLKGGERIISEGIIKARPGTQVSIMPSGGEQPRDGAAKK

Sequences:

>Translated_371_residues
MTSRQLFSLPLALALCLALVACDSEKKAQQGAMHLPVAIFDVVARDEPWPAEYQAQASGSRAVEVRARVEAIIEKRLYEE
GDYVEQGQLLFQLERDHYEALMQQAQAQFTNAEREWRRVRPLYEKNAVSQKERDNALAAYESAKAAMRQAKINLDYCQVT
APVSGYSSKENYTPGNLVGNNSLLTYVNQTDPMYIDFSIAAPERMTRQNLAAEGRLALPAEGRYKARLRLLDGSMYKGEG
EVTFIDSQVQPSTGVIKARAVFANADRSIMPGQYVRLYMDGDILKNAVLVPQKCVLLTQKGSLVMGVDKDDKVYPIPVVV
GVSVGDKYLVLEGLKGGERIISEGIIKARPGTQVSIMPSGGEQPRDGAAKK
>Mature_370_residues
TSRQLFSLPLALALCLALVACDSEKKAQQGAMHLPVAIFDVVARDEPWPAEYQAQASGSRAVEVRARVEAIIEKRLYEEG
DYVEQGQLLFQLERDHYEALMQQAQAQFTNAEREWRRVRPLYEKNAVSQKERDNALAAYESAKAAMRQAKINLDYCQVTA
PVSGYSSKENYTPGNLVGNNSLLTYVNQTDPMYIDFSIAAPERMTRQNLAAEGRLALPAEGRYKARLRLLDGSMYKGEGE
VTFIDSQVQPSTGVIKARAVFANADRSIMPGQYVRLYMDGDILKNAVLVPQKCVLLTQKGSLVMGVDKDDKVYPIPVVVG
VSVGDKYLVLEGLKGGERIISEGIIKARPGTQVSIMPSGGEQPRDGAAKK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=36.6847826086957, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789665, Length=386, Percent_Identity=33.160621761658, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789929, Length=380, Percent_Identity=30.5263157894737, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI87082036, Length=317, Percent_Identity=25.8675078864353, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40954; Mature: 40823

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRQLFSLPLALALCLALVACDSEKKAQQGAMHLPVAIFDVVARDEPWPAEYQAQASGS
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
RAVEVRARVEAIIEKRLYEEGDYVEQGQLLFQLERDHYEALMQQAQAQFTNAEREWRRVR
CEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHC
PLYEKNAVSQKERDNALAAYESAKAAMRQAKINLDYCQVTAPVSGYSSKENYTPGNLVGN
CHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEECC
NSLLTYVNQTDPMYIDFSIAAPERMTRQNLAAEGRLALPAEGRYKARLRLLDGSMYKGEG
CCEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCEECCCCCCCEEEEEEECCCEECCCC
EVTFIDSQVQPSTGVIKARAVFANADRSIMPGQYVRLYMDGDILKNAVLVPQKCVLLTQK
CEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCHHHHCEEECCCEEEEEEEC
GSLVMGVDKDDKVYPIPVVVGVSVGDKYLVLEGLKGGERIISEGIIKARPGTQVSIMPSG
CCEEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCHHHHHHCCCEECCCCCEEEEECCC
GEQPRDGAAKK
CCCCCCCCCCC
>Mature Secondary Structure 
TSRQLFSLPLALALCLALVACDSEKKAQQGAMHLPVAIFDVVARDEPWPAEYQAQASGS
CCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
RAVEVRARVEAIIEKRLYEEGDYVEQGQLLFQLERDHYEALMQQAQAQFTNAEREWRRVR
CEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHC
PLYEKNAVSQKERDNALAAYESAKAAMRQAKINLDYCQVTAPVSGYSSKENYTPGNLVGN
CHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEECC
NSLLTYVNQTDPMYIDFSIAAPERMTRQNLAAEGRLALPAEGRYKARLRLLDGSMYKGEG
CCEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCEECCCCCCCEEEEEEECCCEECCCC
EVTFIDSQVQPSTGVIKARAVFANADRSIMPGQYVRLYMDGDILKNAVLVPQKCVLLTQK
CEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCHHHHCEEECCCEEEEEEEC
GSLVMGVDKDDKVYPIPVVVGVSVGDKYLVLEGLKGGERIISEGIIKARPGTQVSIMPSG
CCEEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCHHHHHHCCCEECCCCCEEEEECCC
GEQPRDGAAKK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]