Click here to switch to the map view.

The map label for this gene is queA [H]

Identifier: 220903425

GI number: 220903425

Start: 177275

End: 178372

Strand: Reverse

Name: queA [H]

Synonym: Ddes_0139

Alternate gene names: 220903425

Gene position: 178372-177275 (Counterclockwise)

Preceding gene: 220903432

Following gene: 220903424

Centisome position: 6.21

GC content: 62.11

Gene sequence:

>1098_bases
ATGCACATTGAGGAAGCTGATTTCTTACTGCAAAGCTATCGTTTCGACCTGCCGCAGGAGCAGGTCGCCCAGTTTCCACC
TGAAGAACGGGGCGGCTCGCGGCTTCTGGTCATGCCGCGCAACGGCGAGCTGACCCTGCGCCATGAAAATTTTTGTGACC
TGCCGGACTGCCTGCCCGAGGGAGCGTTGCTGATTGCCAACAATTCCCGAGTATTGCAGGCGCGTCTTCTTGGTACACGC
TCCACAGGCGGCAAGGTGGAATTTTTACTGCTCACTCCCCTGCCTCTTGTGATCGAACGGGCCAGAGCCGATAAAAACGG
CGGCCTGTATGCCGAGGCCGAGGGCCTTGTACGCTCCGGCGGCAGCATCCGCGAAGGCGAAAGTTTCGGCTTTGGCGCGG
GCATAGGCGTAACCGTGCTGGAATCCGGCCCCTTCGGGCAGCGGCGCGTGCGCCTGAGCTGGACGGGCGACCTTGCGGCG
GCCTTTGCGGCCACGGGCCATATACCCCTGCCGCCCTACATCAAGCGCCCCGATGCTGAAGACGACCTGAGCCGCTACCA
GACCGTCTATTCCCGCCAGGACAAGACCGGTTCTGTGGCCGCGCCCACGGCCGGGCTGCACTTTACGCCGCCCCTGCGCG
AGCGTCTGGCGGCAAAAGGCTTTGAATGGGCGGAAGTAACGCTGTACGTGGGCTACGGCACGTTCAGCCCTGTGCGCAGC
AGCGACATACGCAGCCACCGCATGCACCGCGAATATATAGAAGTGCCCGAAGCCACGGCCCTGGCAATCGCCAGGGCCAG
GGAAGAAAAAAGGCCGGTCATTGCCGTGGGTACCACAAGCCTGCGAACCCTTGAAGGTGTGGCGGAGCTTTGCGGCCGCG
TGCAGCCCTATACCGGCTGGACGGATATTTTCCTGTATCCCGGCAGGCCCTTCCGCGTGGCGGACGGGCTTGTGACCAAT
TTTCACCTGCCGGAATCCTCCCTGCTCATGCTGGTCTCGGCCTTTGCCGGGCGCAAGCGTGTGCTCGACGCCTATGCCGA
GGCCATTGCCAACAACTACAGATTCTTTTCATACGGGGATGCCATGCTGATCCGCTGA

Upstream 100 bases:

>100_bases
GCAAGGGGAGCAATCACGTCACCCTAGCCTCAACGCCCTGCCCTGACAAGAGCGGCAGGCACGTAAGGGGCGGGAACATC
ATAACCGGGACCTGTACGCC

Downstream 100 bases:

>100_bases
CGCCCCTGCCCGCGCCGTCCGTACTCCGCACGCCGCTTCCGGGCATGCGCAACCTGCGGGCGGCTCACGGCCGGCACACC
CTTGCGGGATGGTCCTGCAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MHIEEADFLLQSYRFDLPQEQVAQFPPEERGGSRLLVMPRNGELTLRHENFCDLPDCLPEGALLIANNSRVLQARLLGTR
STGGKVEFLLLTPLPLVIERARADKNGGLYAEAEGLVRSGGSIREGESFGFGAGIGVTVLESGPFGQRRVRLSWTGDLAA
AFAATGHIPLPPYIKRPDAEDDLSRYQTVYSRQDKTGSVAAPTAGLHFTPPLRERLAAKGFEWAEVTLYVGYGTFSPVRS
SDIRSHRMHREYIEVPEATALAIARAREEKRPVIAVGTTSLRTLEGVAELCGRVQPYTGWTDIFLYPGRPFRVADGLVTN
FHLPESSLLMLVSAFAGRKRVLDAYAEAIANNYRFFSYGDAMLIR

Sequences:

>Translated_365_residues
MHIEEADFLLQSYRFDLPQEQVAQFPPEERGGSRLLVMPRNGELTLRHENFCDLPDCLPEGALLIANNSRVLQARLLGTR
STGGKVEFLLLTPLPLVIERARADKNGGLYAEAEGLVRSGGSIREGESFGFGAGIGVTVLESGPFGQRRVRLSWTGDLAA
AFAATGHIPLPPYIKRPDAEDDLSRYQTVYSRQDKTGSVAAPTAGLHFTPPLRERLAAKGFEWAEVTLYVGYGTFSPVRS
SDIRSHRMHREYIEVPEATALAIARAREEKRPVIAVGTTSLRTLEGVAELCGRVQPYTGWTDIFLYPGRPFRVADGLVTN
FHLPESSLLMLVSAFAGRKRVLDAYAEAIANNYRFFSYGDAMLIR
>Mature_365_residues
MHIEEADFLLQSYRFDLPQEQVAQFPPEERGGSRLLVMPRNGELTLRHENFCDLPDCLPEGALLIANNSRVLQARLLGTR
STGGKVEFLLLTPLPLVIERARADKNGGLYAEAEGLVRSGGSIREGESFGFGAGIGVTVLESGPFGQRRVRLSWTGDLAA
AFAATGHIPLPPYIKRPDAEDDLSRYQTVYSRQDKTGSVAAPTAGLHFTPPLRERLAAKGFEWAEVTLYVGYGTFSPVRS
SDIRSHRMHREYIEVPEATALAIARAREEKRPVIAVGTTSLRTLEGVAELCGRVQPYTGWTDIFLYPGRPFRVADGLVTN
FHLPESSLLMLVSAFAGRKRVLDAYAEAIANNYRFFSYGDAMLIR

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=360, Percent_Identity=45, Blast_Score=277, Evalue=8e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40315; Mature: 40315

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIEEADFLLQSYRFDLPQEQVAQFPPEERGGSRLLVMPRNGELTLRHENFCDLPDCLPE
CCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCCC
GALLIANNSRVLQARLLGTRSTGGKVEFLLLTPLPLVIERARADKNGGLYAEAEGLVRSG
CEEEEECCCEEEEEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEECCHHHHCC
GSIREGESFGFGAGIGVTVLESGPFGQRRVRLSWTGDLAAAFAATGHIPLPPYIKRPDAE
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCH
DDLSRYQTVYSRQDKTGSVAAPTAGLHFTPPLRERLAAKGFEWAEVTLYVGYGTFSPVRS
HHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
SDIRSHRMHREYIEVPEATALAIARAREEKRPVIAVGTTSLRTLEGVAELCGRVQPYTGW
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCC
TDIFLYPGRPFRVADGLVTNFHLPESSLLMLVSAFAGRKRVLDAYAEAIANNYRFFSYGD
EEEEEECCCCEEECCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECC
AMLIR
EEEEC
>Mature Secondary Structure
MHIEEADFLLQSYRFDLPQEQVAQFPPEERGGSRLLVMPRNGELTLRHENFCDLPDCLPE
CCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCCC
GALLIANNSRVLQARLLGTRSTGGKVEFLLLTPLPLVIERARADKNGGLYAEAEGLVRSG
CEEEEECCCEEEEEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEECCHHHHCC
GSIREGESFGFGAGIGVTVLESGPFGQRRVRLSWTGDLAAAFAATGHIPLPPYIKRPDAE
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCH
DDLSRYQTVYSRQDKTGSVAAPTAGLHFTPPLRERLAAKGFEWAEVTLYVGYGTFSPVRS
HHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
SDIRSHRMHREYIEVPEATALAIARAREEKRPVIAVGTTSLRTLEGVAELCGRVQPYTGW
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCC
TDIFLYPGRPFRVADGLVTNFHLPESSLLMLVSAFAGRKRVLDAYAEAIANNYRFFSYGD
EEEEEECCCCEEECCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECC
AMLIR
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA