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The map label for this gene is vipB [H]

Identifier: 220903395

GI number: 220903395

Start: 142663

End: 143685

Strand: Direct

Name: vipB [H]

Synonym: Ddes_0109

Alternate gene names: 220903395

Gene position: 142663-143685 (Clockwise)

Preceding gene: 220903392

Following gene: 220903397

Centisome position: 4.96

GC content: 58.94

Gene sequence:

>1023_bases
ATGAGCGCGTATTCCCAACTGTGTGCCACTATGCAGGCTTCTCCTTCCCGCTGGCTGATTACCGGAGTGGCGGGCTTTAT
CGGTTCCAACCTGCTTGAACGCCTTCTCGGGCTGGGCCAGACCGTTGTGGGGCTGGACAACTTCTTGACCGGGTATCAGA
AAAATCTTGATATGGTCCGTGATATTGTGGGGCCGGAAGCCTGGAGCCGCTTTACCTTCATCGAAGGTGATATTCGTGAT
GTAGACACCTGCCGCAAGGCCTGTGAAGGCGTGCAGCATGTGCTGCATGAAGCCGCGCTCGGTTCTGTGCCGCGCTCCAT
TGATGATCCCTTGCTGTCAAATAGCTGCAATATCACAGGGTTTTTGCACATGCTGGTGGCTGCCCGCGATGCCGGGGTAA
AAAGCTTTGTGTATGCCGCCTCTTCTTCCACCTACGGCGATTCGCCCGAACTGCCCAAGGTGGAAGACAAGATCGGCCGC
CCCCTGTCGCCTTATGCCGTCACCAAGTATGTGGACGAGCTGTACGCCGATGTTTTTACGCGCTGCTACGGTTTTTCCAG
TGTCGGGTTGCGCTACTTCAACGTGTTCGGCCAGCGCCAGGACCCTTACGGAGCTTACGCGGCGGTTATTCCCCAGTGGT
TTGCCAGCCTCATCAAAGGGGAAACCGTCTATGTGAACGGCGATGGCGAAACCAGCCGCGACTTCTGCTATATCGACAAC
GTGGTGGAGGCCAACCTGCTGGCGAGCTTTGCCCGGGGCGAGGCTGCCAATACCGTGTACAACGTGGCTTTTGGGCAACG
CACAACCCTTAACGAACTTTTTGCCCTCATCCGTGAAGAAGTGGCGCGCCACAAGCCCGAAGCCGCCAAGGCCGATGTCG
AGCACCGTGATTTTCGCGCGGGCGACGTGCGCCACAGCCTGGCTGACATCAGCCGCGCCGAGACGCGCCTTGGCTATGCC
CCCCGCTTTGACGTGCGGCAGGGGCTGCGGCTCGCAGGCGACTGGTACGCCGCCAATCTGTAG

Upstream 100 bases:

>100_bases
CGGCCCGGCAGCGTAGGATTATGGCCGCCCGAAAGCGTCCGTCAGCTCTTGCAACGACGGTGCGCGGGCAAAAAATCAAT
GCAAAAAACAGGACGCAAAC

Downstream 100 bases:

>100_bases
AGCCTGTTGCCTTCGGGAATGGACATCTGCGGGTGTGCGTTCCCAGAATTGTGCGCGCCTGCTGTGGCGCGCGGCAGCGT
CGCCGATAGCGTTCCGGTTT

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSAYSQLCATMQASPSRWLITGVAGFIGSNLLERLLGLGQTVVGLDNFLTGYQKNLDMVRDIVGPEAWSRFTFIEGDIRD
VDTCRKACEGVQHVLHEAALGSVPRSIDDPLLSNSCNITGFLHMLVAARDAGVKSFVYAASSSTYGDSPELPKVEDKIGR
PLSPYAVTKYVDELYADVFTRCYGFSSVGLRYFNVFGQRQDPYGAYAAVIPQWFASLIKGETVYVNGDGETSRDFCYIDN
VVEANLLASFARGEAANTVYNVAFGQRTTLNELFALIREEVARHKPEAAKADVEHRDFRAGDVRHSLADISRAETRLGYA
PRFDVRQGLRLAGDWYAANL

Sequences:

>Translated_340_residues
MSAYSQLCATMQASPSRWLITGVAGFIGSNLLERLLGLGQTVVGLDNFLTGYQKNLDMVRDIVGPEAWSRFTFIEGDIRD
VDTCRKACEGVQHVLHEAALGSVPRSIDDPLLSNSCNITGFLHMLVAARDAGVKSFVYAASSSTYGDSPELPKVEDKIGR
PLSPYAVTKYVDELYADVFTRCYGFSSVGLRYFNVFGQRQDPYGAYAAVIPQWFASLIKGETVYVNGDGETSRDFCYIDN
VVEANLLASFARGEAANTVYNVAFGQRTTLNELFALIREEVARHKPEAAKADVEHRDFRAGDVRHSLADISRAETRLGYA
PRFDVRQGLRLAGDWYAANL
>Mature_339_residues
SAYSQLCATMQASPSRWLITGVAGFIGSNLLERLLGLGQTVVGLDNFLTGYQKNLDMVRDIVGPEAWSRFTFIEGDIRDV
DTCRKACEGVQHVLHEAALGSVPRSIDDPLLSNSCNITGFLHMLVAARDAGVKSFVYAASSSTYGDSPELPKVEDKIGRP
LSPYAVTKYVDELYADVFTRCYGFSSVGLRYFNVFGQRQDPYGAYAAVIPQWFASLIKGETVYVNGDGETSRDFCYIDNV
VEANLLASFARGEAANTVYNVAFGQRTTLNELFALIREEVARHKPEAAKADVEHRDFRAGDVRHSLADISRAETRLGYAP
RFDVRQGLRLAGDWYAANL

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=28.0120481927711, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=27.5757575757576, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI56237023, Length=325, Percent_Identity=26.4615384615385, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI56118217, Length=325, Percent_Identity=26.4615384615385, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI189083684, Length=325, Percent_Identity=26.4615384615385, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786974, Length=322, Percent_Identity=27.0186335403727, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=28.7790697674419, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=26.2247838616715, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790049, Length=243, Percent_Identity=26.3374485596708, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=26.647564469914, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=349, Percent_Identity=26.647564469914, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=25.1497005988024, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=26.7062314540059, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=26.7062314540059, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=24.6458923512748, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19923002, Length=355, Percent_Identity=28.7323943661972, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356223, Length=335, Percent_Identity=25.9701492537313, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37504; Mature: 37372

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYSQLCATMQASPSRWLITGVAGFIGSNLLERLLGLGQTVVGLDNFLTGYQKNLDMVR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DIVGPEAWSRFTFIEGDIRDVDTCRKACEGVQHVLHEAALGSVPRSIDDPLLSNSCNITG
HHHCHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHH
FLHMLVAARDAGVKSFVYAASSSTYGDSPELPKVEDKIGRPLSPYAVTKYVDELYADVFT
HHHHHHHHHCCCHHHHHEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
RCYGFSSVGLRYFNVFGQRQDPYGAYAAVIPQWFASLIKGETVYVNGDGETSRDFCYIDN
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEHHH
VVEANLLASFARGEAANTVYNVAFGQRTTLNELFALIREEVARHKPEAAKADVEHRDFRA
HHHHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCH
GDVRHSLADISRAETRLGYAPRFDVRQGLRLAGDWYAANL
HHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHCCCCCCCCC
>Mature Secondary Structure 
SAYSQLCATMQASPSRWLITGVAGFIGSNLLERLLGLGQTVVGLDNFLTGYQKNLDMVR
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DIVGPEAWSRFTFIEGDIRDVDTCRKACEGVQHVLHEAALGSVPRSIDDPLLSNSCNITG
HHHCHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHH
FLHMLVAARDAGVKSFVYAASSSTYGDSPELPKVEDKIGRPLSPYAVTKYVDELYADVFT
HHHHHHHHHCCCHHHHHEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
RCYGFSSVGLRYFNVFGQRQDPYGAYAAVIPQWFASLIKGETVYVNGDGETSRDFCYIDN
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEHHH
VVEANLLASFARGEAANTVYNVAFGQRTTLNELFALIREEVARHKPEAAKADVEHRDFRA
HHHHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCH
GDVRHSLADISRAETRLGYAPRFDVRQGLRLAGDWYAANL
HHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]