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The map label for this gene is lacA [C]

Identifier: 220903364

GI number: 220903364

Start: 102489

End: 103223

Strand: Reverse

Name: lacA [C]

Synonym: Ddes_0078

Alternate gene names: 220903364

Gene position: 103223-102489 (Counterclockwise)

Preceding gene: 220903365

Following gene: 220903363

Centisome position: 3.59

GC content: 66.39

Gene sequence:

>735_bases
ATGAACATGTTGCGCAAAGCTCTGGAACTGGGCCTTACCCCGGCCAATATCATCAGCTACCTGTGCCTTCAGGCCATGAC
GCGCTGGGGCCGCTTCTGGGGGACCCTGCGCCTGCGCTGCAAGGCGGTGTTTGTGGGCGTGCCTGTAGGTCAGGGCGTCA
GCGCGCACGGGCCTGTGGGCCTTATGCGCTGGCCGGGCAGCCATATTTCCATCGGCGCGGGCGCAAGCCTCATATCCTCC
TGGCGGCGGGCCACGGCCGCTGCTCTGGCAAGCCCCGTGCGCCTGCGCACCTTTGGCCCCGGAGCGCGTATAGACGTGGG
TCCGGGCGCGCAGCTCAGCGGCACGTCCATAACGGCCCGCTCCACCACCATCAGCATCGGCCGCCAGGCTTTGCTGGCCC
CAAACTGCATTATCGTGGATTCGGACTTTCACGCTCACTGGCCGCCAGAAGCCCGTGCCACGGAACCGGGCATGGAAGGC
GACAGGCCCGTAACCATCGGCGACTACGCCTGGATCGGCCTGAACTGCATCATCCTCAAAGGCGTCACCATTGGTGAAGG
AGCCATTATCGGCGCGGGCAGCGTTGTAACACGCGATGTGCCGCCCCATTGCCTGGCCGCTGGCTCTCCTGCCCGCGTGC
TGCGGCATCTCGCCCCCGGCGAGGATCGCACTGCGGCCGCGGACCGGACTGTCCCGGCCGAAGACAAGAGCTTTCCCGAA
ACCGCACGGGCTTAG

Upstream 100 bases:

>100_bases
AACCCTTTTTCGGCTGCCTGAAAAAACCGCAGAACATATCCGCTGCCGCGCGGCGGCATGCAGCCGCAGACCGCAGGATA
ACCGCACAAAGGAACATGGC

Downstream 100 bases:

>100_bases
GACGTATCCGTATCATGTCTGTGGGTTCTTACATCGCCGCCTTGCTGGCCTCGGAAAAACTGGGTGGGCAGGTTACCTGC
CACCGTCTGCTGCCTGCGTC

Product: transferase hexapeptide repeat containing protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNMLRKALELGLTPANIISYLCLQAMTRWGRFWGTLRLRCKAVFVGVPVGQGVSAHGPVGLMRWPGSHISIGAGASLISS
WRRATAAALASPVRLRTFGPGARIDVGPGAQLSGTSITARSTTISIGRQALLAPNCIIVDSDFHAHWPPEARATEPGMEG
DRPVTIGDYAWIGLNCIILKGVTIGEGAIIGAGSVVTRDVPPHCLAAGSPARVLRHLAPGEDRTAAADRTVPAEDKSFPE
TARA

Sequences:

>Translated_244_residues
MNMLRKALELGLTPANIISYLCLQAMTRWGRFWGTLRLRCKAVFVGVPVGQGVSAHGPVGLMRWPGSHISIGAGASLISS
WRRATAAALASPVRLRTFGPGARIDVGPGAQLSGTSITARSTTISIGRQALLAPNCIIVDSDFHAHWPPEARATEPGMEG
DRPVTIGDYAWIGLNCIILKGVTIGEGAIIGAGSVVTRDVPPHCLAAGSPARVLRHLAPGEDRTAAADRTVPAEDKSFPE
TARA
>Mature_244_residues
MNMLRKALELGLTPANIISYLCLQAMTRWGRFWGTLRLRCKAVFVGVPVGQGVSAHGPVGLMRWPGSHISIGAGASLISS
WRRATAAALASPVRLRTFGPGARIDVGPGAQLSGTSITARSTTISIGRQALLAPNCIIVDSDFHAHWPPEARATEPGMEG
DRPVTIGDYAWIGLNCIILKGVTIGEGAIIGAGSVVTRDVPPHCLAAGSPARVLRHLAPGEDRTAAADRTVPAEDKSFPE
TARA

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=136, Percent_Identity=34.5588235294118, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786664, Length=144, Percent_Identity=32.6388888888889, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788345, Length=133, Percent_Identity=30.8270676691729, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=125, Percent_Identity=34.4, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 25611; Mature: 25611

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMLRKALELGLTPANIISYLCLQAMTRWGRFWGTLRLRCKAVFVGVPVGQGVSAHGPVG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEE
LMRWPGSHISIGAGASLISSWRRATAAALASPVRLRTFGPGARIDVGPGAQLSGTSITAR
EEECCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCEEEEC
STTISIGRQALLAPNCIIVDSDFHAHWPPEARATEPGMEGDRPVTIGDYAWIGLNCIILK
CEEEHHCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCEEEECEEEEEEE
GVTIGEGAIIGAGSVVTRDVPPHCLAAGSPARVLRHLAPGEDRTAAADRTVPAEDKSFPE
CEEECCCCEEECCCEEECCCCCHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
TARA
HHCC
>Mature Secondary Structure
MNMLRKALELGLTPANIISYLCLQAMTRWGRFWGTLRLRCKAVFVGVPVGQGVSAHGPVG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEE
LMRWPGSHISIGAGASLISSWRRATAAALASPVRLRTFGPGARIDVGPGAQLSGTSITAR
EEECCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCEEEEC
STTISIGRQALLAPNCIIVDSDFHAHWPPEARATEPGMEGDRPVTIGDYAWIGLNCIILK
CEEEHHCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCEEEECEEEEEEE
GVTIGEGAIIGAGSVVTRDVPPHCLAAGSPARVLRHLAPGEDRTAAADRTVPAEDKSFPE
CEEECCCCEEECCCEEECCCCCHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
TARA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]