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The map label for this gene is panB [H]

Identifier: 220903361

GI number: 220903361

Start: 97190

End: 98023

Strand: Direct

Name: panB [H]

Synonym: Ddes_0075

Alternate gene names: 220903361

Gene position: 97190-98023 (Clockwise)

Preceding gene: 220903360

Following gene: 220903369

Centisome position: 3.38

GC content: 60.31

Gene sequence:

>834_bases
ATGAAAAACACTGTGGTCACCTTTCGTGCCGCCAAGGGGCAGGACAAGCTTGTTGTGCTTACCGCCTATGACTACAGCAC
GGCCCGCGTCATGGATGCCGCCGGGGTCAACGCTCTGCTGGTAGGGGATTCTCTCGGCATGGTCATGCTGGGGCATGAGG
ATACGCTAGGCGTCAGCATGGACGACATGGTCCGTCATTGTGGCGCAGTGGCACGCGGCGCGGAGCAGGCCCTCGTTATC
TGCGATATGCCTTATATGAGTTACCACACAGGAATTGATGAGGCTGTGCGCAATGCCGGAAGGCTGGTGACGGAAGGGCG
CGCCCAGGCCGTCAAGCTGGAAGGCGGCGCGGAATTCTGCGATGTGGTGAGGGCCATTGTACGGGCTTCCATTCCTGTCA
TGGGGCATCTTGGCCTTACGCCCCAGTCTGTGAACGCCATGGGTGGCTTCAAGATGCAGGGTAAGACTCCGGCAGCGGCC
CAGAAACTGCTGGACGACGCCCGCGCCCTTCAGGAGGCCGGAGCCTTTGCGCTGGTGCTCGAATGCGTACCCGCCCCGCT
GGCTGCCCGTATTACAAAGGAACTTGCCATTCCTGTCATCGGCATCGGCGCAGGGGCCGACTGCGATGGCCAGGTACTGG
TCTGGCAGGACATGGCGGGCCTCAATGCCCGTCCGCTGCCACGCTTTGTAAAATATTTCGGCTCGGTGGACATGGCCCTG
CGCGATGCCATCGAAGCCTATACCCGTGAGGTGCGCGTGGGGGCTTTTCCCGGTGAGGAACATTGCTATCCCCTGCCCGA
AGGTATGGAAAAAGCCCTGAAAAAGCTTAAATAG

Upstream 100 bases:

>100_bases
CAAGGCGCTGGCGGGACGCTCGTGTCCGGCAGGGCGGCATTTCCGGCGTTCACGACGCCTTCGGCATCTCCGGCAGGATT
TTTTTACAAGGAGCAAAGAA

Downstream 100 bases:

>100_bases
GCCCGGCATGCGGCGGCCTGCCGTACAGGCGCCAACAGGCGCTATGCACCAACTGCATACACAGAACCCCGGCACTGCCG
GGGTTCTGTCATTTCTTTCG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKNTVVTFRAAKGQDKLVVLTAYDYSTARVMDAAGVNALLVGDSLGMVMLGHEDTLGVSMDDMVRHCGAVARGAEQALVI
CDMPYMSYHTGIDEAVRNAGRLVTEGRAQAVKLEGGAEFCDVVRAIVRASIPVMGHLGLTPQSVNAMGGFKMQGKTPAAA
QKLLDDARALQEAGAFALVLECVPAPLAARITKELAIPVIGIGAGADCDGQVLVWQDMAGLNARPLPRFVKYFGSVDMAL
RDAIEAYTREVRVGAFPGEEHCYPLPEGMEKALKKLK

Sequences:

>Translated_277_residues
MKNTVVTFRAAKGQDKLVVLTAYDYSTARVMDAAGVNALLVGDSLGMVMLGHEDTLGVSMDDMVRHCGAVARGAEQALVI
CDMPYMSYHTGIDEAVRNAGRLVTEGRAQAVKLEGGAEFCDVVRAIVRASIPVMGHLGLTPQSVNAMGGFKMQGKTPAAA
QKLLDDARALQEAGAFALVLECVPAPLAARITKELAIPVIGIGAGADCDGQVLVWQDMAGLNARPLPRFVKYFGSVDMAL
RDAIEAYTREVRVGAFPGEEHCYPLPEGMEKALKKLK
>Mature_277_residues
MKNTVVTFRAAKGQDKLVVLTAYDYSTARVMDAAGVNALLVGDSLGMVMLGHEDTLGVSMDDMVRHCGAVARGAEQALVI
CDMPYMSYHTGIDEAVRNAGRLVTEGRAQAVKLEGGAEFCDVVRAIVRASIPVMGHLGLTPQSVNAMGGFKMQGKTPAAA
QKLLDDARALQEAGAFALVLECVPAPLAARITKELAIPVIGIGAGADCDGQVLVWQDMAGLNARPLPRFVKYFGSVDMAL
RDAIEAYTREVRVGAFPGEEHCYPLPEGMEKALKKLK

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=265, Percent_Identity=46.7924528301887, Blast_Score=232, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6319653, Length=261, Percent_Identity=34.8659003831418, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 29464; Mature: 29464

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTVVTFRAAKGQDKLVVLTAYDYSTARVMDAAGVNALLVGDSLGMVMLGHEDTLGVSM
CCCCEEEEEECCCCCCEEEEEECCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCH
DDMVRHCGAVARGAEQALVICDMPYMSYHTGIDEAVRNAGRLVTEGRAQAVKLEGGAEFC
HHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHCCCEEECCCCEEEEECCCHHHH
DVVRAIVRASIPVMGHLGLTPQSVNAMGGFKMQGKTPAAAQKLLDDARALQEAGAFALVL
HHHHHHHHHCCCCEECCCCCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEEE
ECVPAPLAARITKELAIPVIGIGAGADCDGQVLVWQDMAGLNARPLPRFVKYFGSVDMAL
HHCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHH
RDAIEAYTREVRVGAFPGEEHCYPLPEGMEKALKKLK
HHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKNTVVTFRAAKGQDKLVVLTAYDYSTARVMDAAGVNALLVGDSLGMVMLGHEDTLGVSM
CCCCEEEEEECCCCCCEEEEEECCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCH
DDMVRHCGAVARGAEQALVICDMPYMSYHTGIDEAVRNAGRLVTEGRAQAVKLEGGAEFC
HHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHCCCEEECCCCEEEEECCCHHHH
DVVRAIVRASIPVMGHLGLTPQSVNAMGGFKMQGKTPAAAQKLLDDARALQEAGAFALVL
HHHHHHHHHCCCCEECCCCCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEEE
ECVPAPLAARITKELAIPVIGIGAGADCDGQVLVWQDMAGLNARPLPRFVKYFGSVDMAL
HHCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHH
RDAIEAYTREVRVGAFPGEEHCYPLPEGMEKALKKLK
HHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]