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The map label for this gene is uvrD [H]

Identifier: 220903357

GI number: 220903357

Start: 91530

End: 93836

Strand: Reverse

Name: uvrD [H]

Synonym: Ddes_0071

Alternate gene names: 220903357

Gene position: 93836-91530 (Counterclockwise)

Preceding gene: 220903362

Following gene: 220903356

Centisome position: 3.27

GC content: 62.55

Gene sequence:

>2307_bases
ATGATTGATTACGCTCAAGCGCTTAACGAAGCCCAATATGAAGCCGCCACCAGCGGCGACGGCCCAGTGCTGGTGGTAGC
CGGGGCAGGCAGCGGCAAAACACGCACCATTGTTTACCGTCTGGCATGGCTGGCGGAAAACGGTGTTTCGCCCGAATCCA
TGCTGCTGCTTACCTTTACCCGCAAGGCCGCGCAGGAAATGCTGCACCGCGCGGGACTGCTGCTGGACCACGGCCTTTCC
GGCGTGCAGGGGGGAACCTTTCACGCCTTTGCCTTCAGCGTGCTGCGCCGCTGGAAACCCGCATGGCTGGCCGACCGCCC
CTTTACGGTCATGGATGCGGCAGACATTACTTCAGCCGTAAAGCACTGCCGCGACGACCTGAAGCTCGGCAAGGGCGACA
AGTCCTTTCCTAAAACACAGACCGTGGTGGGGCTGCTCAGCAAGGCCCGCAACAAGGAGCTGCCGCTGGACGAGGTGCTC
AGGCGCGAAGCCTTTCACCTGCTGCCCCACGCCGAAAGCCTGAGCAGGCTGGGCGAGGCCTACAATACCTACCGACGCGA
AAAAGGCCTCATGGACTATGACGACCTGCTCTTTGAGCTTGAGGCCCTGCTGCGGCAGAATGAAGTTGCCGCCGCCAGTC
TGCGGCAGCGCTTCAGCCATATCCTTGTGGACGAATATCAGGACACAAACCTGGTGCAGGCCCGTATTGTGCGGTTGCTG
GCCGGGCCGGAGGACGCCCCCCCCGGCAATGTGATGGCCGTGGGCGACGAGGCGCAGTCCATCTATGCGTTTCGTGGGGC
CAACGTGCGTAATATTCTTGATTTTCCCAAGCTTTTTCCCGGTGCGCGCGTCATCCGCCTGGAGGAGAACTACCGCTCCA
CCAGGCCCGTACTTGAGGTCGCCAACAGCCTGCTGGCCCACGCGGCGGAGTCATTCCGCAAAAAACTGTTCACCCGCAAG
GAAGGAGGCGAACCGGTCCGGCTGGTAACCCCCCTGAGCGATGCCAGCCAGGCGCGCCTTGTGGTACGCCGCATTGAAGA
ACTGCTGCGCGACCACATGCCTCATGAAATCGCGGTACTTTTTCGCGCGGGCTTTCATTCGTACAACCTTGAAATGGCGC
TCAACCAGGCCGGCATAGGCTTTCGCAAATACGGCGGCCTGCGCTATACCGAGGCGGCCCACGTCAAGGACGTGATGGCC
TATGCACGGCTGCTGCTCAACCCGCTGGACCTGCCCGCCTTTGCCCGTGTGGCCGCCCAGCACAGCGGCATTGGCCCAAA
GACGGTAGAAAAGCTGTACAACGTGATCCGCAGCGGCGACGCTGCCGCCACTGAAAAGGCCCTGGCCCGCCACACGGGCT
TTCTGGAAGATGTACGCTTCATCAATGACCTGCGCACACGCCCCATGGCGCCGTCTTCCACCCTGGCCGCCGTGCTGGAG
CATTATCGCCCGCGCCTGGAGAGCCTGTACCCCGAAGACTGGCCGCGCCGCCAGCAGGGGCTTGAAGAAATCATCCAGAT
GGCTTCGGGCTATGTTCACCTGGACCTTTTTCTGGCCGACCTGGCACTGGAATCACCCGAAGAGGATGAAAACGACGCCG
AGGGCAAGATCACCCTTTCCACCGTACACTCGGCCAAAGGCCTTGAATGGAACGCTGTGTGCATCATTGACCTTGTGGAA
GACCGTTTTCCCTCGCGCCATGCCCTTGCCCGGCCCGAAGACTTTGAAGAAGAACGCCGCCTCATGTACGTGGCCTGCAC
CCGCGCCCGCCAGTGCCTCGACCTCTACGCTCCGGCCTCGCTGTACAACAGGGCCGAGCGCGGCAGCCAGCATGTGAACC
AGAGCCCCTTTGTGCGCGAACTGGCCCCAGGCCTGGTGGAAGAATGGGTAGAAGGCTTCGGCGGCGGCATTTCACGCCGG
GCTGCCGGTGGATTCGGCTCGCCGGGCGCGTTCCAGCCCCGGCCCGCCACGCGGCCGCAAGGCATGCAGCCCCCACAGGA
GCATGCAGCCTATGACGACTGCCAGTTACCGCCGGAGCATGCCGCAGGCGGGCGCCCCGTTGCCGCCGGCGGGCCTGCCG
GGAATGGCGGGTATGGCGGATACAGCAGGCCCGAAGTACCGCCCCCAGCCGCAACGTCCTCACAGGGCGGGGACACTCCC
GGTCAGGGGCATCTGTGCTACTGCCAGCACCGCATTTTTGGCCGGGGCAAAATAATCAGGCACATCTCGCCCGAAAAGGT
GCAGGTGAATTTTCCCGGTTTTGGACTCAAGATCATTCTGAGCGAATATCTGCTCATGGAGAACTGA

Upstream 100 bases:

>100_bases
CACAGAAGTTCACGAACGCCAGGCAGTGCTGCTTCAGCCGCGCATGGCCGCGCTTTCGCCGCACCCCGGTCCGGCTGCCG
ACTTACTCCAAGGTTGTACC

Downstream 100 bases:

>100_bases
TATGGTCCCGTCCCCTGTTCCGGGCGGCTCTGCCGCCACCAGCGCGGTCCCACTTTCAGAAGATCGCATCCTGGCCTGCC
TGGCGGGATACTTTCCGCAG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 768; Mature: 768

Protein sequence:

>768_residues
MIDYAQALNEAQYEAATSGDGPVLVVAGAGSGKTRTIVYRLAWLAENGVSPESMLLLTFTRKAAQEMLHRAGLLLDHGLS
GVQGGTFHAFAFSVLRRWKPAWLADRPFTVMDAADITSAVKHCRDDLKLGKGDKSFPKTQTVVGLLSKARNKELPLDEVL
RREAFHLLPHAESLSRLGEAYNTYRREKGLMDYDDLLFELEALLRQNEVAAASLRQRFSHILVDEYQDTNLVQARIVRLL
AGPEDAPPGNVMAVGDEAQSIYAFRGANVRNILDFPKLFPGARVIRLEENYRSTRPVLEVANSLLAHAAESFRKKLFTRK
EGGEPVRLVTPLSDASQARLVVRRIEELLRDHMPHEIAVLFRAGFHSYNLEMALNQAGIGFRKYGGLRYTEAAHVKDVMA
YARLLLNPLDLPAFARVAAQHSGIGPKTVEKLYNVIRSGDAAATEKALARHTGFLEDVRFINDLRTRPMAPSSTLAAVLE
HYRPRLESLYPEDWPRRQQGLEEIIQMASGYVHLDLFLADLALESPEEDENDAEGKITLSTVHSAKGLEWNAVCIIDLVE
DRFPSRHALARPEDFEEERRLMYVACTRARQCLDLYAPASLYNRAERGSQHVNQSPFVRELAPGLVEEWVEGFGGGISRR
AAGGFGSPGAFQPRPATRPQGMQPPQEHAAYDDCQLPPEHAAGGRPVAAGGPAGNGGYGGYSRPEVPPPAATSSQGGDTP
GQGHLCYCQHRIFGRGKIIRHISPEKVQVNFPGFGLKIILSEYLLMEN

Sequences:

>Translated_768_residues
MIDYAQALNEAQYEAATSGDGPVLVVAGAGSGKTRTIVYRLAWLAENGVSPESMLLLTFTRKAAQEMLHRAGLLLDHGLS
GVQGGTFHAFAFSVLRRWKPAWLADRPFTVMDAADITSAVKHCRDDLKLGKGDKSFPKTQTVVGLLSKARNKELPLDEVL
RREAFHLLPHAESLSRLGEAYNTYRREKGLMDYDDLLFELEALLRQNEVAAASLRQRFSHILVDEYQDTNLVQARIVRLL
AGPEDAPPGNVMAVGDEAQSIYAFRGANVRNILDFPKLFPGARVIRLEENYRSTRPVLEVANSLLAHAAESFRKKLFTRK
EGGEPVRLVTPLSDASQARLVVRRIEELLRDHMPHEIAVLFRAGFHSYNLEMALNQAGIGFRKYGGLRYTEAAHVKDVMA
YARLLLNPLDLPAFARVAAQHSGIGPKTVEKLYNVIRSGDAAATEKALARHTGFLEDVRFINDLRTRPMAPSSTLAAVLE
HYRPRLESLYPEDWPRRQQGLEEIIQMASGYVHLDLFLADLALESPEEDENDAEGKITLSTVHSAKGLEWNAVCIIDLVE
DRFPSRHALARPEDFEEERRLMYVACTRARQCLDLYAPASLYNRAERGSQHVNQSPFVRELAPGLVEEWVEGFGGGISRR
AAGGFGSPGAFQPRPATRPQGMQPPQEHAAYDDCQLPPEHAAGGRPVAAGGPAGNGGYGGYSRPEVPPPAATSSQGGDTP
GQGHLCYCQHRIFGRGKIIRHISPEKVQVNFPGFGLKIILSEYLLMEN
>Mature_768_residues
MIDYAQALNEAQYEAATSGDGPVLVVAGAGSGKTRTIVYRLAWLAENGVSPESMLLLTFTRKAAQEMLHRAGLLLDHGLS
GVQGGTFHAFAFSVLRRWKPAWLADRPFTVMDAADITSAVKHCRDDLKLGKGDKSFPKTQTVVGLLSKARNKELPLDEVL
RREAFHLLPHAESLSRLGEAYNTYRREKGLMDYDDLLFELEALLRQNEVAAASLRQRFSHILVDEYQDTNLVQARIVRLL
AGPEDAPPGNVMAVGDEAQSIYAFRGANVRNILDFPKLFPGARVIRLEENYRSTRPVLEVANSLLAHAAESFRKKLFTRK
EGGEPVRLVTPLSDASQARLVVRRIEELLRDHMPHEIAVLFRAGFHSYNLEMALNQAGIGFRKYGGLRYTEAAHVKDVMA
YARLLLNPLDLPAFARVAAQHSGIGPKTVEKLYNVIRSGDAAATEKALARHTGFLEDVRFINDLRTRPMAPSSTLAAVLE
HYRPRLESLYPEDWPRRQQGLEEIIQMASGYVHLDLFLADLALESPEEDENDAEGKITLSTVHSAKGLEWNAVCIIDLVE
DRFPSRHALARPEDFEEERRLMYVACTRARQCLDLYAPASLYNRAERGSQHVNQSPFVRELAPGLVEEWVEGFGGGISRR
AAGGFGSPGAFQPRPATRPQGMQPPQEHAAYDDCQLPPEHAAGGRPVAAGGPAGNGGYGGYSRPEVPPPAATSSQGGDTP
GQGHLCYCQHRIFGRGKIIRHISPEKVQVNFPGFGLKIILSEYLLMEN

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=658, Percent_Identity=34.8024316109423, Blast_Score=296, Evalue=5e-81,
Organism=Escherichia coli, GI48994965, Length=635, Percent_Identity=28.8188976377953, Blast_Score=202, Evalue=8e-53,
Organism=Escherichia coli, GI1787196, Length=370, Percent_Identity=25.9459459459459, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789183, Length=145, Percent_Identity=31.7241379310345, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322369, Length=505, Percent_Identity=25.7425742574257, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84927; Mature: 84927

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYAQALNEAQYEAATSGDGPVLVVAGAGSGKTRTIVYRLAWLAENGVSPESMLLLTFT
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHEEEHHHH
RKAAQEMLHRAGLLLDHGLSGVQGGTFHAFAFSVLRRWKPAWLADRPFTVMDAADITSAV
HHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHH
KHCRDDLKLGKGDKSFPKTQTVVGLLSKARNKELPLDEVLRREAFHLLPHAESLSRLGEA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
YNTYRREKGLMDYDDLLFELEALLRQNEVAAASLRQRFSHILVDEYQDTNLVQARIVRLL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGPEDAPPGNVMAVGDEAQSIYAFRGANVRNILDFPKLFPGARVIRLEENYRSTRPVLEV
CCCCCCCCCCEEEECCCHHHEEHCCCCCHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHH
ANSLLAHAAESFRKKLFTRKEGGEPVRLVTPLSDASQARLVVRRIEELLRDHMPHEIAVL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
FRAGFHSYNLEMALNQAGIGFRKYGGLRYTEAAHVKDVMAYARLLLNPLDLPAFARVAAQ
HHHCCCCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HSGIGPKTVEKLYNVIRSGDAAATEKALARHTGFLEDVRFINDLRTRPMAPSSTLAAVLE
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHH
HYRPRLESLYPEDWPRRQQGLEEIIQMASGYVHLDLFLADLALESPEEDENDAEGKITLS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEEE
TVHSAKGLEWNAVCIIDLVEDRFPSRHALARPEDFEEERRLMYVACTRARQCLDLYAPAS
EECCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHCCHH
LYNRAERGSQHVNQSPFVRELAPGLVEEWVEGFGGGISRRAAGGFGSPGAFQPRPATRPQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCC
GMQPPQEHAAYDDCQLPPEHAAGGRPVAAGGPAGNGGYGGYSRPEVPPPAATSSQGGDTP
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GQGHLCYCQHRIFGRGKIIRHISPEKVQVNFPGFGLKIILSEYLLMEN
CCCCEEEEEHHHHCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDYAQALNEAQYEAATSGDGPVLVVAGAGSGKTRTIVYRLAWLAENGVSPESMLLLTFT
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHEEEHHHH
RKAAQEMLHRAGLLLDHGLSGVQGGTFHAFAFSVLRRWKPAWLADRPFTVMDAADITSAV
HHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHH
KHCRDDLKLGKGDKSFPKTQTVVGLLSKARNKELPLDEVLRREAFHLLPHAESLSRLGEA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
YNTYRREKGLMDYDDLLFELEALLRQNEVAAASLRQRFSHILVDEYQDTNLVQARIVRLL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGPEDAPPGNVMAVGDEAQSIYAFRGANVRNILDFPKLFPGARVIRLEENYRSTRPVLEV
CCCCCCCCCCEEEECCCHHHEEHCCCCCHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHH
ANSLLAHAAESFRKKLFTRKEGGEPVRLVTPLSDASQARLVVRRIEELLRDHMPHEIAVL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
FRAGFHSYNLEMALNQAGIGFRKYGGLRYTEAAHVKDVMAYARLLLNPLDLPAFARVAAQ
HHHCCCCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HSGIGPKTVEKLYNVIRSGDAAATEKALARHTGFLEDVRFINDLRTRPMAPSSTLAAVLE
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHH
HYRPRLESLYPEDWPRRQQGLEEIIQMASGYVHLDLFLADLALESPEEDENDAEGKITLS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEEE
TVHSAKGLEWNAVCIIDLVEDRFPSRHALARPEDFEEERRLMYVACTRARQCLDLYAPAS
EECCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHCCHH
LYNRAERGSQHVNQSPFVRELAPGLVEEWVEGFGGGISRRAAGGFGSPGAFQPRPATRPQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCC
GMQPPQEHAAYDDCQLPPEHAAGGRPVAAGGPAGNGGYGGYSRPEVPPPAATSSQGGDTP
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GQGHLCYCQHRIFGRGKIIRHISPEKVQVNFPGFGLKIILSEYLLMEN
CCCCEEEEEHHHHCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]