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The map label for this gene is yfhQ [H]

Identifier: 220903350

GI number: 220903350

Start: 84087

End: 85394

Strand: Reverse

Name: yfhQ [H]

Synonym: Ddes_0064

Alternate gene names: 220903350

Gene position: 85394-84087 (Counterclockwise)

Preceding gene: 220903354

Following gene: 220903348

Centisome position: 2.97

GC content: 61.77

Gene sequence:

>1308_bases
ATGCCCAAAAAAGCCATAAAATTCCCCACAGATCCAAAAACAGGCAAAAAATCCCGCACCACTCCCAACCCGCTGCCCGG
CCTGGAAACGGCCGCCGCGGCGCAGGGCTGTTCGGGCCTACCGCCGCGGCATCTTCCGCCGGAAAAACACATTCCCGCCC
TGCAAAATGCCCTGCTGGACTGGTTTGCCGTGCATCAGCGCGCCCTGCCCTGGCGGAACAGCTACACACCCTATGAAGTC
TGGATTTCAGAGGTCATGCTGCAACAGACGCAGATGGAACGCGGGGTAAGGTATTTTATACGCTGGATGGAGCGCTTCCC
CGACATTGCCGCGCTGGCAGCCGCCCATGAGGAGGACGTGCTGCGCATGTGGGAAGGGCTGGGCTACTATTCGCGCGCGC
GCCACATTCTTGCGGCGGCCCGCAAAATCATGGCCGAGCACAACGGAATATTCCCCCGCGATCCTGCGGCCATACGCGCC
CTGCCCGGTGTCGGCCCCTATACGGCCGGAGCCATTGCCAGCATTGCCTTTGAAAAAAAGCTGCCCTGCGTTGATGCCAA
TGTGGAGCGTGTTGTTTCACGCATTTTTGATGTGGACAGCCCGGTCAAGCAGGAACCTGCCGCGGGCGTCATCCACCAGT
GGGCACTCAGGCTCGTTCCCGAGGGCAAGGCCCGCGCCCACAATCAGGCCATGATGGAGCTTGGCGCGCTTGTATGCCGT
AAAAAACCGTGCTGCGCGTCCTGTCCGCTGGGGGCGTTCTGCATAAGCCACCACCTGGGCATTGAGGATCAACGCCCGGT
TCCGGGCAAACGGGCCACGGTTACCCCTGTCAACGCCGTCACCGGCGTGCTGCGCCGCGGAGAAAAAATTTTTGTGCAAA
AGCGCCCGGCTTCGGGTGTGTGGGGCAATTTGTGGGAATTTCCCGGCGGCCGCGTAGAAGAAGGCGAAAGCCCTGAACAG
GCCGTTGTGCGTGAGTTTATGGAAGAAACAGGCTTTACCGTAAACGTGGCAGCCCGTCATGGCATCATCCGCCATGGCTA
TACCACCTACCGGCTCACCCTGCATTGCTTCGGCCTTGATCTGGCCGCCCCAGGCAAGGATTGTACGCCGGAGACATGCC
CGGCCCCGCCGGTGCTCACCGCTGCCACGCAATACCGCTGGGCAAGCCCGCAGGAACTGGAAGACCTCGCCATGCCTGCG
GCCCATCGCAAGCTGGCAGACAGTCTTTTCAGCCAGCCCGGGCAAACGGCGGGGGCATCAACAATGCCGCGCCAGGGCCG
CCTCACGGACGGAACGGACACAGACTGA

Upstream 100 bases:

>100_bases
GTGGCGGCGCGCTCCGGCAAAGGCCGGAATGCGCCCGATCCGGAATATAGGCCATGCCCCGGCAGATGACAATGCCCGCC
ACCGGGTATAGACTGCCCGC

Downstream 100 bases:

>100_bases
CCGGACCGCCCGCGAGGGACTGGCGATAAAAGATGCAGATCATTCCTGCAGACGCGCCGTCAGGTCGGCCAGCCGCTCAA
TGAGGGCCACGAGCCTGTCC

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPKKAIKFPTDPKTGKKSRTTPNPLPGLETAAAAQGCSGLPPRHLPPEKHIPALQNALLDWFAVHQRALPWRNSYTPYEV
WISEVMLQQTQMERGVRYFIRWMERFPDIAALAAAHEEDVLRMWEGLGYYSRARHILAAARKIMAEHNGIFPRDPAAIRA
LPGVGPYTAGAIASIAFEKKLPCVDANVERVVSRIFDVDSPVKQEPAAGVIHQWALRLVPEGKARAHNQAMMELGALVCR
KKPCCASCPLGAFCISHHLGIEDQRPVPGKRATVTPVNAVTGVLRRGEKIFVQKRPASGVWGNLWEFPGGRVEEGESPEQ
AVVREFMEETGFTVNVAARHGIIRHGYTTYRLTLHCFGLDLAAPGKDCTPETCPAPPVLTAATQYRWASPQELEDLAMPA
AHRKLADSLFSQPGQTAGASTMPRQGRLTDGTDTD

Sequences:

>Translated_435_residues
MPKKAIKFPTDPKTGKKSRTTPNPLPGLETAAAAQGCSGLPPRHLPPEKHIPALQNALLDWFAVHQRALPWRNSYTPYEV
WISEVMLQQTQMERGVRYFIRWMERFPDIAALAAAHEEDVLRMWEGLGYYSRARHILAAARKIMAEHNGIFPRDPAAIRA
LPGVGPYTAGAIASIAFEKKLPCVDANVERVVSRIFDVDSPVKQEPAAGVIHQWALRLVPEGKARAHNQAMMELGALVCR
KKPCCASCPLGAFCISHHLGIEDQRPVPGKRATVTPVNAVTGVLRRGEKIFVQKRPASGVWGNLWEFPGGRVEEGESPEQ
AVVREFMEETGFTVNVAARHGIIRHGYTTYRLTLHCFGLDLAAPGKDCTPETCPAPPVLTAATQYRWASPQELEDLAMPA
AHRKLADSLFSQPGQTAGASTMPRQGRLTDGTDTD
>Mature_434_residues
PKKAIKFPTDPKTGKKSRTTPNPLPGLETAAAAQGCSGLPPRHLPPEKHIPALQNALLDWFAVHQRALPWRNSYTPYEVW
ISEVMLQQTQMERGVRYFIRWMERFPDIAALAAAHEEDVLRMWEGLGYYSRARHILAAARKIMAEHNGIFPRDPAAIRAL
PGVGPYTAGAIASIAFEKKLPCVDANVERVVSRIFDVDSPVKQEPAAGVIHQWALRLVPEGKARAHNQAMMELGALVCRK
KPCCASCPLGAFCISHHLGIEDQRPVPGKRATVTPVNAVTGVLRRGEKIFVQKRPASGVWGNLWEFPGGRVEEGESPEQA
VVREFMEETGFTVNVAARHGIIRHGYTTYRLTLHCFGLDLAAPGKDCTPETCPAPPVLTAATQYRWASPQELEDLAMPAA
HRKLADSLFSQPGQTAGASTMPRQGRLTDGTDTD

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8- oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal effici

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HhH domain [H]

Homologues:

Organism=Homo sapiens, GI115298654, Length=417, Percent_Identity=32.1342925659472, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI115298652, Length=417, Percent_Identity=32.1342925659472, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI115298650, Length=417, Percent_Identity=32.1342925659472, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI115298648, Length=417, Percent_Identity=32.1342925659472, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI6912520, Length=417, Percent_Identity=32.1342925659472, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI190358497, Length=417, Percent_Identity=32.1342925659472, Blast_Score=185, Evalue=8e-47,
Organism=Escherichia coli, GI1789331, Length=302, Percent_Identity=36.7549668874172, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787920, Length=128, Percent_Identity=32.8125, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 47845; Mature: 47714

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00893 NUDIX ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKAIKFPTDPKTGKKSRTTPNPLPGLETAAAAQGCSGLPPRHLPPEKHIPALQNALLD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
WFAVHQRALPWRNSYTPYEVWISEVMLQQTQMERGVRYFIRWMERFPDIAALAAAHEEDV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LRMWEGLGYYSRARHILAAARKIMAEHNGIFPRDPAAIRALPGVGPYTAGAIASIAFEKK
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHC
LPCVDANVERVVSRIFDVDSPVKQEPAAGVIHQWALRLVPEGKARAHNQAMMELGALVCR
CCCCCCCHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KKPCCASCPLGAFCISHHLGIEDQRPVPGKRATVTPVNAVTGVLRRGEKIFVQKRPASGV
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEEECCCCCC
WGNLWEFPGGRVEEGESPEQAVVREFMEETGFTVNVAARHGIIRHGYTTYRLTLHCFGLD
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCHHHCCCEEEEEEEEEEECE
LAAPGKDCTPETCPAPPVLTAATQYRWASPQELEDLAMPAAHRKLADSLFSQPGQTAGAS
ECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
TMPRQGRLTDGTDTD
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKKAIKFPTDPKTGKKSRTTPNPLPGLETAAAAQGCSGLPPRHLPPEKHIPALQNALLD
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
WFAVHQRALPWRNSYTPYEVWISEVMLQQTQMERGVRYFIRWMERFPDIAALAAAHEEDV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LRMWEGLGYYSRARHILAAARKIMAEHNGIFPRDPAAIRALPGVGPYTAGAIASIAFEKK
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHC
LPCVDANVERVVSRIFDVDSPVKQEPAAGVIHQWALRLVPEGKARAHNQAMMELGALVCR
CCCCCCCHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KKPCCASCPLGAFCISHHLGIEDQRPVPGKRATVTPVNAVTGVLRRGEKIFVQKRPASGV
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEEECCCCCC
WGNLWEFPGGRVEEGESPEQAVVREFMEETGFTVNVAARHGIIRHGYTTYRLTLHCFGLD
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCHHHCCCEEEEEEEEEEECE
LAAPGKDCTPETCPAPPVLTAATQYRWASPQELEDLAMPAAHRKLADSLFSQPGQTAGAS
ECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
TMPRQGRLTDGTDTD
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]