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The map label for this gene is gadA [H]

Identifier: 220903331

GI number: 220903331

Start: 59454

End: 60860

Strand: Reverse

Name: gadA [H]

Synonym: Ddes_0045

Alternate gene names: 220903331

Gene position: 60860-59454 (Counterclockwise)

Preceding gene: 220903332

Following gene: 220903329

Centisome position: 2.12

GC content: 59.99

Gene sequence:

>1407_bases
ATGACTGCGAAAAACACCTTGCGCAATACGGTACTTGATGACACCTATGCCGCGTCGGATATGGCAAATGCCATGCCGCG
CCATGAGATGCCCCAACAGGAAAGCCGCCCCCGCGACGTATACCAGGCCATTCATGATGAGCTTATGCTGGACGGCAATT
CTCGCCAGAACCTGGCGACGTTCTGCCAGACCTGGGTAGACCCCGAGATCAACCAGATCATGGCCGAGTGCGTTGACAAA
AACATGATCGACAAGGACGAATACCCGCAGACTGCCGAAACAGAAACACGCTGCGTGCACATGCTGGCCGACCTGTGGCA
TTCGCCGGACCCCAAGGGAACCCTAGGCTGCTCCACCACCGGTTCCAGCGAAGCCGCCATGCTTGGCGGGCTTGCCATGA
AGCGCCGCTGGGTCAACATGCGCAAGGCCGCCGGAAAGCCCTATGACAAACCCAACATGGTGTGCGGCCCCGTGCAGGTC
TGCTGGGAAAAATTCGCCCGCTACTGGGACGTGGAACTGCGCGAAATTCCTATGGAAAAAGACCGCCTGATCATGACCGC
CGAAGAGGTCATCAAGCGTTGCGATGAAAATACCATCGGCGTTGTGCCCACCCTTGGCGTGACCTTTACCGGCCAGTATG
AGCCGGTGGAAGAAGTAAGCAAAGCCCTCGACAAACTGCAGAAGGACAAGGGCTGGGACATTCCCATTCATGTGGACGGC
GCCAGCGGCGGCTTTCTTGCCCCGTTCATCGAGCCGGACCTGCTCTGGGATTTCCGCCTGCCCCGCGTCAAGTCCATCAA
CTCTTCCGGTCACAAATTCGGCCTTGCCCCGCTTGGCGTGGGCTGGGTAGTGTGGCGCGAAAAAAGCGACCTGCCCGAAG
ACCTGATTTTCAATGTGAACTACCTGGGCGGCAACATGCCCACCTTTGCGCTCAACTTCTCCCGCCCCGGCGGCCAGATC
ATCGCCCAGTACTACAACTTCCTGCGTCTGGGACGCGAAGGCTACCGCCGCATTCACCAGAACTGCTACGATACGGCCCG
CTTCCTGGGCGACGAAATCGCCAAGCTCGGTCCCTTTGAAGTGCTGTACAACGGGCGCGGCGGCATCCCGGCCCTGTGCT
GGACCTTCAAGGCCAATGCCAAGACCAGCTACAGCCTCTATGACCTCTCGGACCGTCTGCGCTCGCGCGGCTGGCAGGTG
CCTGCCTACTCCATGCCCGCTCACCGCGAAGACCTCGTGGTCATGCGCGTACTGGTACGCCACGGCTTCAGCCGTGACCT
CGGCTCCCTGCTTGTCGATGACCTCAAGCGGGCCATGGCCCACTTTGATGCACACCCCGTCAGCAAACCCCTGAGCGAAA
CCGAAGCCGGCAGCAACAGCCACGCCGGACGCGCTGCAAAAAAATAA

Upstream 100 bases:

>100_bases
CATTCCGCTCATCATCCAGGCCTTCAAAAAGCCTCAATGGAAACGCACAACAAGTGCGGAAAACAATTTTTCAATTAACG
GCAACAATGGAGATTAAAGC

Downstream 100 bases:

>100_bases
CCTCCTGCGCACAATGATAAGGGCCGCCCCCGTGCTTACGGGGGCGGCCCTTCAGTATTGGCAAAACCGCAATGCGCTTT
GCGCAACGTCTATTTCAGGT

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTAKNTLRNTVLDDTYAASDMANAMPRHEMPQQESRPRDVYQAIHDELMLDGNSRQNLATFCQTWVDPEINQIMAECVDK
NMIDKDEYPQTAETETRCVHMLADLWHSPDPKGTLGCSTTGSSEAAMLGGLAMKRRWVNMRKAAGKPYDKPNMVCGPVQV
CWEKFARYWDVELREIPMEKDRLIMTAEEVIKRCDENTIGVVPTLGVTFTGQYEPVEEVSKALDKLQKDKGWDIPIHVDG
ASGGFLAPFIEPDLLWDFRLPRVKSINSSGHKFGLAPLGVGWVVWREKSDLPEDLIFNVNYLGGNMPTFALNFSRPGGQI
IAQYYNFLRLGREGYRRIHQNCYDTARFLGDEIAKLGPFEVLYNGRGGIPALCWTFKANAKTSYSLYDLSDRLRSRGWQV
PAYSMPAHREDLVVMRVLVRHGFSRDLGSLLVDDLKRAMAHFDAHPVSKPLSETEAGSNSHAGRAAKK

Sequences:

>Translated_468_residues
MTAKNTLRNTVLDDTYAASDMANAMPRHEMPQQESRPRDVYQAIHDELMLDGNSRQNLATFCQTWVDPEINQIMAECVDK
NMIDKDEYPQTAETETRCVHMLADLWHSPDPKGTLGCSTTGSSEAAMLGGLAMKRRWVNMRKAAGKPYDKPNMVCGPVQV
CWEKFARYWDVELREIPMEKDRLIMTAEEVIKRCDENTIGVVPTLGVTFTGQYEPVEEVSKALDKLQKDKGWDIPIHVDG
ASGGFLAPFIEPDLLWDFRLPRVKSINSSGHKFGLAPLGVGWVVWREKSDLPEDLIFNVNYLGGNMPTFALNFSRPGGQI
IAQYYNFLRLGREGYRRIHQNCYDTARFLGDEIAKLGPFEVLYNGRGGIPALCWTFKANAKTSYSLYDLSDRLRSRGWQV
PAYSMPAHREDLVVMRVLVRHGFSRDLGSLLVDDLKRAMAHFDAHPVSKPLSETEAGSNSHAGRAAKK
>Mature_467_residues
TAKNTLRNTVLDDTYAASDMANAMPRHEMPQQESRPRDVYQAIHDELMLDGNSRQNLATFCQTWVDPEINQIMAECVDKN
MIDKDEYPQTAETETRCVHMLADLWHSPDPKGTLGCSTTGSSEAAMLGGLAMKRRWVNMRKAAGKPYDKPNMVCGPVQVC
WEKFARYWDVELREIPMEKDRLIMTAEEVIKRCDENTIGVVPTLGVTFTGQYEPVEEVSKALDKLQKDKGWDIPIHVDGA
SGGFLAPFIEPDLLWDFRLPRVKSINSSGHKFGLAPLGVGWVVWREKSDLPEDLIFNVNYLGGNMPTFALNFSRPGGQII
AQYYNFLRLGREGYRRIHQNCYDTARFLGDEIAKLGPFEVLYNGRGGIPALCWTFKANAKTSYSLYDLSDRLRSRGWQVP
AYSMPAHREDLVVMRVLVRHGFSRDLGSLLVDDLKRAMAHFDAHPVSKPLSETEAGSNSHAGRAAKK

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=333, Percent_Identity=25.5255255255255, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1787769, Length=454, Percent_Identity=56.1674008810573, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1789934, Length=454, Percent_Identity=55.9471365638767, Blast_Score=551, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI17557272, Length=376, Percent_Identity=25.531914893617, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17543922, Length=336, Percent_Identity=25, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25148342, Length=326, Percent_Identity=25.1533742331288, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323905, Length=485, Percent_Identity=39.5876288659794, Blast_Score=321, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6320500, Length=409, Percent_Identity=24.9388753056235, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355963, Length=349, Percent_Identity=23.7822349570201, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24654344, Length=349, Percent_Identity=23.7822349570201, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52797; Mature: 52666

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKNTLRNTVLDDTYAASDMANAMPRHEMPQQESRPRDVYQAIHDELMLDGNSRQNLAT
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHH
FCQTWVDPEINQIMAECVDKNMIDKDEYPQTAETETRCVHMLADLWHSPDPKGTLGCSTT
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GSSEAAMLGGLAMKRRWVNMRKAAGKPYDKPNMVCGPVQVCWEKFARYWDVELREIPMEK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHCCCCC
DRLIMTAEEVIKRCDENTIGVVPTLGVTFTGQYEPVEEVSKALDKLQKDKGWDIPIHVDG
CCEEECHHHHHHHCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
ASGGFLAPFIEPDLLWDFRLPRVKSINSSGHKFGLAPLGVGWVVWREKSDLPEDLIFNVN
CCCCEECCCCCCCCEEECCCCCHHCCCCCCCEECCCCCCCCEEEEECCCCCCHHHEEEEE
YLGGNMPTFALNFSRPGGQIIAQYYNFLRLGREGYRRIHQNCYDTARFLGDEIAKLGPFE
ECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VLYNGRGGIPALCWTFKANAKTSYSLYDLSDRLRSRGWQVPAYSMPAHREDLVVMRVLVR
EEECCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
HGFSRDLGSLLVDDLKRAMAHFDAHPVSKPLSETEAGSNSHAGRAAKK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAKNTLRNTVLDDTYAASDMANAMPRHEMPQQESRPRDVYQAIHDELMLDGNSRQNLAT
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHH
FCQTWVDPEINQIMAECVDKNMIDKDEYPQTAETETRCVHMLADLWHSPDPKGTLGCSTT
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GSSEAAMLGGLAMKRRWVNMRKAAGKPYDKPNMVCGPVQVCWEKFARYWDVELREIPMEK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHCCCCC
DRLIMTAEEVIKRCDENTIGVVPTLGVTFTGQYEPVEEVSKALDKLQKDKGWDIPIHVDG
CCEEECHHHHHHHCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
ASGGFLAPFIEPDLLWDFRLPRVKSINSSGHKFGLAPLGVGWVVWREKSDLPEDLIFNVN
CCCCEECCCCCCCCEEECCCCCHHCCCCCCCEECCCCCCCCEEEEECCCCCCHHHEEEEE
YLGGNMPTFALNFSRPGGQIIAQYYNFLRLGREGYRRIHQNCYDTARFLGDEIAKLGPFE
ECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VLYNGRGGIPALCWTFKANAKTSYSLYDLSDRLRSRGWQVPAYSMPAHREDLVVMRVLVR
EEECCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
HGFSRDLGSLLVDDLKRAMAHFDAHPVSKPLSETEAGSNSHAGRAAKK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]