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The map label for this gene is rsmB [H]

Identifier: 220903300

GI number: 220903300

Start: 19079

End: 20659

Strand: Direct

Name: rsmB [H]

Synonym: Ddes_0014

Alternate gene names: 220903300

Gene position: 19079-20659 (Clockwise)

Preceding gene: 220903299

Following gene: 220903302

Centisome position: 0.66

GC content: 59.14

Gene sequence:

>1581_bases
ATGAGCAGGAACAGGAACATATCGTTGCGTAAGCTGTCGTGTAACGGCCGCACGGCTGCCCTGCGGGCGCTGTTGCTGGT
TGATGACGGGCTGAGCGCCCAGCAGGCCCTTGCATCTGTTCTGGACGCCCCTGTGGCGCACGGGGCTTCAGGCAACTCGC
GCGAAAAGAGCCACACCGCAAATGCCGGCAGATGCCAGGGGCTTTCAGCGCAGGACAGGGCGCTGTGCACCGAGCTTGTA
TATGGCTGCCTGCGTACCGAACTGCGCCTGAATTTTCTGTTGGGCAGGGTTTTGCCACGGCCCCAGGGTCTGCCGCGGCC
CATGCAGATACTGCTGGCGCTGGCAGTATACGGCCTGCTGTTTCAGGACAAGGTTCCGTCCCATGCTGTGGTTTATGAAG
CCGTGGAACAGGCAAGAGCACTTTTTGGCCAGGGGCTGGCCCGGGTCGCCAACGGCGCATTGCGATCTTTGCAGCGCATG
GGGGAAGAACCGGCGCAACCATCCTTTTATGCGGGGCAGTCGGGTGTAAAAAAATACCGGCATGGCGCGGGCGGCACAGA
CAGTTTTTTGGGAAAATGTATCTTTTATTCCATCCCCTTGTGGATTGGTGAGCTTTGGCGTTCCGCCTATGGTGACGAGG
CGGCCTTGAGGCTTATGCAACGCTCTTTTGAACGTCCCTGGGCCGCATTGCGGATCAATGCCCGCCATACCGATGGTGCA
GCTCTGCACGCGGTTTTGGCGGCTCCGCAGGCGGAAGGGCGCGCATCTGCGATAGCCGGATTATCCGGCGCGGCCACAGA
AAGCGGTGCTACAGAACAAGATTCTTTACAATATCATATTGATATTGCAGATAGAGTAGATAAGAATGAAAATAATGAGG
GTCAGGGCAACGGCCATAAGCCCGTTGCCATTGGGCAGTGGGGTCTTGCCTTCGCACCCGGAACCATGCCGCGCGAGGCC
CTTGGCCTTGACCTGAGGCAATGGCTGGCACGGGGCGCATTAAGCTGGCAATCAGCCGGGTCTCAGCAGGCTTTGCTCGC
TCTTGGGCTGGACCGCTGGCATGAACCTGTGTGGGATGCCTGCGCCGGGCATGGCGGCAAAAGCACGGCCCTGATGGAGT
GGGGTGTGCCGGTGAGTTTGTGCACAGACCGTTCTTTTGCGCGTTTGCGGGGGCTGCCCACCCAGTGCACAACACTGGGA
TTGCCAGTGCCGCCGCACTGCCTGGCTGATGCCATCCGACCTCCGGTAGGGCAATGGCCCGGCCATATCCTGGTGGACGC
GCCCTGTTCCGGTCTGGGAGTGCTGGGCCGAAGGCCGGATATCCGCAGGCGGGAACCGCAACATCTTGCTGAACTTGAAG
TCCTGCAACAAAACATGCTGCGCGTGCTTGCCGACATATTGCAGCCCGGCCGGGAACTGGCCTATATAACCTGTACCCTC
AATCCGGCTGAAAACGAACGTGCTGTGGACCAACTGCTTGAAAATAATTCTGATCTGACAATTGAACGCCAGTGGCAGAC
AGGGCACAGCCATCCCTGGCTTGAGGGAATGTTTGGCGCTGTACTGCGCAAAAAAGGCTGA

Upstream 100 bases:

>100_bases
TGCGGCTGTTTCTTGGGCTTGAACATATGGCGGCACACGGACAGGCAGGCGAAAAATGCCCGTCTGTCGATGCGGTAATA
CCTGATGGAGAGGGCCGAGC

Downstream 100 bases:

>100_bases
AAGGTAAAAATGGGGCAGGGCATTCGGGCTGTTACAAAAAACGTAAGGCGGATTGTGCTATAAGCCATAACTGGCGTAGG
TATATGGCAAAAAACAGGCT

Product: NusB/RsmB/TIM44

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSRNRNISLRKLSCNGRTAALRALLLVDDGLSAQQALASVLDAPVAHGASGNSREKSHTANAGRCQGLSAQDRALCTELV
YGCLRTELRLNFLLGRVLPRPQGLPRPMQILLALAVYGLLFQDKVPSHAVVYEAVEQARALFGQGLARVANGALRSLQRM
GEEPAQPSFYAGQSGVKKYRHGAGGTDSFLGKCIFYSIPLWIGELWRSAYGDEAALRLMQRSFERPWAALRINARHTDGA
ALHAVLAAPQAEGRASAIAGLSGAATESGATEQDSLQYHIDIADRVDKNENNEGQGNGHKPVAIGQWGLAFAPGTMPREA
LGLDLRQWLARGALSWQSAGSQQALLALGLDRWHEPVWDACAGHGGKSTALMEWGVPVSLCTDRSFARLRGLPTQCTTLG
LPVPPHCLADAIRPPVGQWPGHILVDAPCSGLGVLGRRPDIRRREPQHLAELEVLQQNMLRVLADILQPGRELAYITCTL
NPAENERAVDQLLENNSDLTIERQWQTGHSHPWLEGMFGAVLRKKG

Sequences:

>Translated_526_residues
MSRNRNISLRKLSCNGRTAALRALLLVDDGLSAQQALASVLDAPVAHGASGNSREKSHTANAGRCQGLSAQDRALCTELV
YGCLRTELRLNFLLGRVLPRPQGLPRPMQILLALAVYGLLFQDKVPSHAVVYEAVEQARALFGQGLARVANGALRSLQRM
GEEPAQPSFYAGQSGVKKYRHGAGGTDSFLGKCIFYSIPLWIGELWRSAYGDEAALRLMQRSFERPWAALRINARHTDGA
ALHAVLAAPQAEGRASAIAGLSGAATESGATEQDSLQYHIDIADRVDKNENNEGQGNGHKPVAIGQWGLAFAPGTMPREA
LGLDLRQWLARGALSWQSAGSQQALLALGLDRWHEPVWDACAGHGGKSTALMEWGVPVSLCTDRSFARLRGLPTQCTTLG
LPVPPHCLADAIRPPVGQWPGHILVDAPCSGLGVLGRRPDIRRREPQHLAELEVLQQNMLRVLADILQPGRELAYITCTL
NPAENERAVDQLLENNSDLTIERQWQTGHSHPWLEGMFGAVLRKKG
>Mature_525_residues
SRNRNISLRKLSCNGRTAALRALLLVDDGLSAQQALASVLDAPVAHGASGNSREKSHTANAGRCQGLSAQDRALCTELVY
GCLRTELRLNFLLGRVLPRPQGLPRPMQILLALAVYGLLFQDKVPSHAVVYEAVEQARALFGQGLARVANGALRSLQRMG
EEPAQPSFYAGQSGVKKYRHGAGGTDSFLGKCIFYSIPLWIGELWRSAYGDEAALRLMQRSFERPWAALRINARHTDGAA
LHAVLAAPQAEGRASAIAGLSGAATESGATEQDSLQYHIDIADRVDKNENNEGQGNGHKPVAIGQWGLAFAPGTMPREAL
GLDLRQWLARGALSWQSAGSQQALLALGLDRWHEPVWDACAGHGGKSTALMEWGVPVSLCTDRSFARLRGLPTQCTTLGL
PVPPHCLADAIRPPVGQWPGHILVDAPCSGLGVLGRRPDIRRREPQHLAELEVLQQNMLRVLADILQPGRELAYITCTLN
PAENERAVDQLLENNSDLTIERQWQTGHSHPWLEGMFGAVLRKKG

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Escherichia coli, GI2367212, Length=441, Percent_Identity=26.7573696145125, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: 2.1.1.-

Molecular weight: Translated: 57033; Mature: 56902

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNRNISLRKLSCNGRTAALRALLLVDDGLSAQQALASVLDAPVAHGASGNSREKSHTA
CCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCC
NAGRCQGLSAQDRALCTELVYGCLRTELRLNFLLGRVLPRPQGLPRPMQILLALAVYGLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FQDKVPSHAVVYEAVEQARALFGQGLARVANGALRSLQRMGEEPAQPSFYAGQSGVKKYR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
HGAGGTDSFLGKCIFYSIPLWIGELWRSAYGDEAALRLMQRSFERPWAALRINARHTDGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCH
ALHAVLAAPQAEGRASAIAGLSGAATESGATEQDSLQYHIDIADRVDKNENNEGQGNGHK
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCC
PVAIGQWGLAFAPGTMPREALGLDLRQWLARGALSWQSAGSQQALLALGLDRWHEPVWDA
CEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCHHHHH
CAGHGGKSTALMEWGVPVSLCTDRSFARLRGLPTQCTTLGLPVPPHCLADAIRPPVGQWP
HCCCCCCCCEEEECCCCCEECCCCHHHHHCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCC
GHILVDAPCSGLGVLGRRPDIRRREPQHLAELEVLQQNMLRVLADILQPGRELAYITCTL
CCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
NPAENERAVDQLLENNSDLTIERQWQTGHSHPWLEGMFGAVLRKKG
CCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRNRNISLRKLSCNGRTAALRALLLVDDGLSAQQALASVLDAPVAHGASGNSREKSHTA
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCC
NAGRCQGLSAQDRALCTELVYGCLRTELRLNFLLGRVLPRPQGLPRPMQILLALAVYGLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FQDKVPSHAVVYEAVEQARALFGQGLARVANGALRSLQRMGEEPAQPSFYAGQSGVKKYR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
HGAGGTDSFLGKCIFYSIPLWIGELWRSAYGDEAALRLMQRSFERPWAALRINARHTDGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCH
ALHAVLAAPQAEGRASAIAGLSGAATESGATEQDSLQYHIDIADRVDKNENNEGQGNGHK
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCC
PVAIGQWGLAFAPGTMPREALGLDLRQWLARGALSWQSAGSQQALLALGLDRWHEPVWDA
CEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCHHHHH
CAGHGGKSTALMEWGVPVSLCTDRSFARLRGLPTQCTTLGLPVPPHCLADAIRPPVGQWP
HCCCCCCCCEEEECCCCCEECCCCHHHHHCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCC
GHILVDAPCSGLGVLGRRPDIRRREPQHLAELEVLQQNMLRVLADILQPGRELAYITCTL
CCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
NPAENERAVDQLLENNSDLTIERQWQTGHSHPWLEGMFGAVLRKKG
CCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]