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The map label for this gene is recF [H]

Identifier: 21911391

GI number: 21911391

Start: 1884820

End: 1885926

Strand: Direct

Name: recF [H]

Synonym: SpyM3_1855

Alternate gene names: 21911391

Gene position: 1884820-1885926 (Clockwise)

Preceding gene: 21911390

Following gene: 21911395

Centisome position: 99.17

GC content: 37.4

Gene sequence:

>1107_bases
ATGTGGATTAAAGAGTTAGAGTTAAAACATTATCGCAATTATGACCAGCTGCTTGCCTCATTTTCGTCGGGTTTAAATGT
CTTTATCGGTAATAATGCTCAAGGGAAAACCAATTTTCTCGAGGCAATCTATTTTCTATCACTTACTAGAAGTCAGCGCA
CGAGGGCTGATAAAGAATTGATTCATTTTGATCACTCAACTGTTTCTCTTACTGGTAAGATTCAGCGTATTAGTGGTACT
GTTGATTTAGAAATCAATCTATCAGATAAAGGACGTGTGACAAAGATTAATGCGCTGAAGCAAGCAAAATTATCTGATTA
TATTGGAACGATGATGGTTGTGCTTTTTGCACCAGAAGATTTGCAACTAGTTAAGGGAGCACCTAGCCTTCGTCGCAAAT
TTATCGACATTGACCTTGGTCAAATTAAGCCTGTTTACTTATCTGAGTTATCCCATTATAACCACGTGCTTAAACAGCGT
AATAGCTATTTAAAAAGTGCCCAACAAATTGATGCCGCTTTTCTAGCTGTTCTAGATGAGCAATTAGCTAGCTATGGAGC
CCGTGTCATGGAACATCGTATTGATTTTATCAATGCGTTAGAGAAAGAAGCAAATACTCATCACCAAGCCATCTCAAATG
GTTTAGAAAGTCTCTCTCTCTCCTATCAGTCGTCAGTTGTCTTTGACAAAAAAACTAATATTTACCAACAATTTTTACAC
CAACTTGAAAAAAATCATCAGAAAGATTTCTTCCGTAAAAACACTAGCGTTGGTCCACATCGCGATGACTTAGCCTTTTA
CATTAACGGGATGAATGCTAACTTTGCTAGTCAAGGTCAGCACCGCAGCCTTATTCTTTCCTTAAAAATGGCTGAAGTTA
GTTTAATGAAGGCCTTGACTGGAGATAATCCCATTCTTTTACTAGACGATGTCATGAGCGAGCTAGACAATACACGACAA
ACAAAGTTACTTGAAACAGTAATTAAAGAAAATGTCCAAACCTTTATCACAACAACAAGTTTGGATCACCTGTCACAGTT
GCCAGAAGGTATCCGTATTTTTCACGTAACAAAAGGAACCGTTCAAATTGATTCTGATATTCACTAA

Upstream 100 bases:

>100_bases
AAACATAACAATCGTCAATCTACTACCAAAAAATCACTAAGAGCAACTAAGAAAACTAAAGGTAAACCAACTGCACCTGT
CCGCTTCCCAGGTATCTAGT

Downstream 100 bases:

>100_bases
TAACATCAAAAAAGGGTTCAGTTGAACTGAGCCTTTTTTTGATATTATGTGGCGATTAAGATTTGACCACACCTAATAAA
ATAGCACCTACGATAAAACA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MWIKELELKHYRNYDQLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSQRTRADKELIHFDHSTVSLTGKIQRISGT
VDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
QLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQ
TKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH

Sequences:

>Translated_368_residues
MWIKELELKHYRNYDQLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSQRTRADKELIHFDHSTVSLTGKIQRISGT
VDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
QLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQ
TKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH
>Mature_368_residues
MWIKELELKHYRNYDQLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSQRTRADKELIHFDHSTVSLTGKIQRISGT
VDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
QLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQ
TKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=365, Percent_Identity=26.027397260274, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41808; Mature: 41808

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIKELELKHYRNYDQLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSQRTRADKEL
CCCHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPED
HEECCCEEEEEEEEEEECCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHEEECCCC
LQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQRNSYLKSAQQIDAAFLAVLDE
HHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
QLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALT
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHC
GDNPILLLDDVMSELDNTRQTKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGT
CCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCE
VQIDSDIH
EEECCCCC
>Mature Secondary Structure
MWIKELELKHYRNYDQLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSQRTRADKEL
CCCHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPED
HEECCCEEEEEEEEEEECCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHEEECCCC
LQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQRNSYLKSAQQIDAAFLAVLDE
HHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
QLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALT
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHC
GDNPILLLDDVMSELDNTRQTKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGT
CCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCE
VQIDSDIH
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]