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The map label for this gene is folD [H]

Identifier: 21910693

GI number: 21910693

Start: 1181431

End: 1182285

Strand: Reverse

Name: folD [H]

Synonym: SpyM3_1157

Alternate gene names: 21910693

Gene position: 1182285-1181431 (Counterclockwise)

Preceding gene: 21910694

Following gene: 21910692

Centisome position: 62.21

GC content: 39.88

Gene sequence:

>855_bases
ATGACAGAACTAATTGATGGTAAAGCCTTAGCTCAAAAGATGCAACAAGAGTTAGCAGCTAAAGTCAACAATCTAAAACA
AAAAAAAGGAATTGTACCAGGCTTAGCCGTTATTCTTGTAGGTGATGATCCTGCTAGTCAGGTGTATGTCCGTAATAAAG
AGCGTGCAGCTCTTACTGTAGGTTTTAAAAGTGAGACCGTTAGATTATCAGAATTCATTTGTCAAGAAGAGCTTATTGCA
GTAATCGAACGTTACAATGCAGATAACACTATTCATGGTATTTTAGTGCAGCTACCCCTGCCAAATCATATTAATGATAA
AAAAATTATTCTCGCCATTGATCCCAAAAAAGATGTGGATGGTTTTCACCCGATGAATACAGGTCACCTTTGGTCAGGAC
GTCCCTTAATGGTTCCTTGTACTCCATCAGGGATTATGGAATTGCTTCGAGAATATAATGTTAACCTTGAAGGTAAACAT
GCCGTCATTATTGGCAGATCGAATATCGTTGGAAAACCAATGGCACAGCTCTTACTAGACAAAAATGCAACAGTCACGTT
GACACATTCAAGAACACGTCAATTAGAAGAAGTATGTCGCTGTGCAGATGTGTTGATTGTGGCAATCGGACAAGGTCATT
TCATAACAAAACAATATATAAAAGAGGGTGCGATAGTGATTGATGTGGGAATGAACCGTGATGATAATGGCAAGCTAATT
GGAGATGTGGCCTTTGATGAGGTGGCAGAAGTTGCAGCGAAAATCACCCCTGTACCAGGAGGTGTCGGTCCTATGACGAT
TGCTATGTTGCTAGAGCAAACTTATCAATCTGCTCTCCGTAGTACTCATAAATAG

Upstream 100 bases:

>100_bases
TTAATCGACTCAAACCAATACTAAAAAATAAGTAAGAGTATAAAGAACAGGCATCTATTTTTTTGTTATAATAGAAAAAT
GAAATTGAAGGGAGATGTCA

Downstream 100 bases:

>100_bases
TTATCATTAGACAATTCAACATAAGAGAATATAAGAAGTCAGCAATGGCTTCCTTTTTTGTTTTTCTGCTCACTATTTAT
TCAGAAAAATGGTATAATTG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPASQVYVRNKERAALTVGFKSETVRLSEFICQEELIA
VIERYNADNTIHGILVQLPLPNHINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKEGAIVIDVGMNRDDNGKLI
GDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRSTHK

Sequences:

>Translated_284_residues
MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPASQVYVRNKERAALTVGFKSETVRLSEFICQEELIA
VIERYNADNTIHGILVQLPLPNHINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKEGAIVIDVGMNRDDNGKLI
GDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRSTHK
>Mature_283_residues
TELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAV
IERYNADNTIHGILVQLPLPNHINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKHA
VIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKEGAIVIDVGMNRDDNGKLIG
DVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRSTHK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=45.3924914675768, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI94721354, Length=290, Percent_Identity=44.4827586206897, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI222418558, Length=290, Percent_Identity=43.448275862069, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=27.6381909547739, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=51.985559566787, Blast_Score=281, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=39.5189003436426, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=45.1048951048951, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=42.0338983050847, Blast_Score=236, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=27.3311897106109, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=44.7457627118644, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=44.7457627118644, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=44.4444444444444, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=44.4444444444444, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=44.4444444444444, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=44.4444444444444, Blast_Score=232, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31097; Mature: 30966

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPASQVYVRNKERAALTV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCEEEEE
GFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPNHINDKKIILAIDPKKDVD
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCC
GFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKHAVIIGRSNIVGKPMAQLLLD
CCCCCCCCCEECCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHC
KNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKEGAIVIDVGMNRDDNGKLI
CCCEEEEECHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCEEEEECCCCCCCCCEE
GDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRSTHK
EHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPASQVYVRNKERAALTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCEEEEE
GFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPNHINDKKIILAIDPKKDVD
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCC
GFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKHAVIIGRSNIVGKPMAQLLLD
CCCCCCCCCEECCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHC
KNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKEGAIVIDVGMNRDDNGKLI
CCCEEEEECHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCEEEEECCCCCCCCCEE
GDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRSTHK
EHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]