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The map label for this gene is recN [H]

Identifier: 21910687

GI number: 21910687

Start: 1175913

End: 1177574

Strand: Reverse

Name: recN [H]

Synonym: SpyM3_1151

Alternate gene names: 21910687

Gene position: 1177574-1175913 (Counterclockwise)

Preceding gene: 21910688

Following gene: 21910686

Centisome position: 61.96

GC content: 39.35

Gene sequence:

>1662_bases
ATGTTATTAGAAATTTCTATTAAAAATTTTGCTATTATTGATGAAATTTCCCTAAATTTTGAAAATGGTATGACGGTTTT
GACGGGTGAAACTGGAGCAGGAAAGTCTATTATCATTGATGCTATGAATATGATGCTTGGTGCGCGTGCTAGTACAGAAG
TGATTCGTCGTGGAGCTAATAAAGCAGAAATTGAGGGGTTCTTTTCGGTAGATGCTACCCCAGAACTGGTTGCGTGTCTG
GAATCATCAGGTATTGCTATGGAAGAAGAACTGATTATTCGCCGAGATATCTTTGCTAATGGCAGAAGCGTGAGCCGCAT
TAATGGTCAGATGGTTAACCTAGCCACCTTAAAACAGGTCGGACAGTTTTTGGTAGATATTCATGGGCAACATGACCAAG
AAGAATTAATGCGACCACAGCTCCACCAGCAAATTTTGGACGCTTTTGGTGATAAGGCTTTTGAGCAATTGAAAGAGAAC
TATCAGCTTATTTTTGATCGTTATAAAAGCTTGCGTCGCCAGGTTATTGACAAACAAAAAAATGAAAAGGAACACAAAGA
TCGTATTGATATGTTGGCCTTTCAGATAGCAGAAATTGAAGCTGCTGCCTTGAGTCGAGGTGAGGATGACCGGTTAAATC
AAGAACGTGATCGCTTAATGAACCACAAACAAATTGCTGATACCCTGACCAATGCCTACGTCATGCTAGATAATGACGAT
TTTTCAAGTCTATCCAATATTCGCTCTAGCATGAATGACTTACTATCAATTGAGCAGTTTGATTCAGAGTACAAAGGGAT
GTCGACTTCGATTTCTGAAGCCTATTATATTCTTGAAGAAGTGAGCAAGCAATTATCAGATACCATTGACCAACTGGATT
TTGATGGTGGGCGATTACAGGAAATTGAATTTCGCTTGGATACACTCAATAGCTTAACCCGTAAATACGGTGGTAATGTG
AATGATGTGCTTGACTACTACGATAATATTATCAAAGAATACCAGCTATTAACAGGAGACGACTTGTCTTCAGGGGATCT
AGAATCAGAATTGAAAGCCTTAGAAAAACAATTGGTTGCTGCAGCCACTAACTTGAGTGAACGCCGCCATCAATTGGCAG
AGCAATTAGAAGCTGAGATTAAAGCTGAACTTAAGGAACTTTATATGGAAAAAGCAGATTTCAAAGTTCACTTTACCACA
TCAAAATTCAATCGTGATGGTAATGAAAGCTTGGAATTTTACATCTCTACTAATCCAGGCGAAGGATTCAAGCCCCTTGT
CAAGGTGGCTTCTGGAGGAGAGTTATCACGCCTTATGTTAGCCATCAAGGCTGCTATTTCTAGAAAAGAAGATAAGACGA
GTATTGTTTTTGATGAAGTAGATACAGGTGTTTCTGGTCGCGTTGCGCAAGCCATTGCCCAAAAAATTTATAAAATTGGG
CGACATGGGCAAGTTCTCGCTATTTCACACTTACCTCAAGTTATTGCTATTGCTGATTATCAGTATTTTATTTCTAAAGA
AAGTAAAGAAGAGTCCACCGTTTCTAAGGTAAGGTTATTAACTCCTGAAGAACGTGTGGAAGAAATTGCTAGCATGATTG
CAGGAACAGATATGACACAAGCTGCTCTTACGCAGGCTCGTGAACTACTTGCCAAACATTAA

Upstream 100 bases:

>100_bases
ATTGTTAATTGCAAAGTCTCCTTCAGAAGCAGATATGATACGCCAAGAAATTTTGCTTTGGATGCAGGGTATAACCTAGA
AAAAAATAGGGGAGAAAGTG

Downstream 100 bases:

>100_bases
GATTATGTTTGGTATTTGATATTTTCATAATTAGATGACATTGATTTTTTAGGTACTAAAATAATCATTGTGTCAGACCA
TTTTTCCTCTGTTAATCTGG

Product: putative DNA repair and genetic recombination protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGANKAEIEGFFSVDATPELVACL
ESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNV
NDVLDYYDNIIKEYQLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFTT
SKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH

Sequences:

>Translated_553_residues
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGANKAEIEGFFSVDATPELVACL
ESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNV
NDVLDYYDNIIKEYQLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFTT
SKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH
>Mature_553_residues
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGANKAEIEGFFSVDATPELVACL
ESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNV
NDVLDYYDNIIKEYQLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFTT
SKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=563, Percent_Identity=34.4582593250444, Blast_Score=331, Evalue=9e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 62286; Mature: 62286

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGAN
CEEEEEECCEEEEEHEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC
KAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQV
HHHCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCCCEEHHHHHHHH
GQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKENYQLIFDRYKSLRRQVIDKQK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCC
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEYQLLTGDDLSSGDLESELKALEKQLVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFTTSKFNRDGNESLEFYISTNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCEEEEEECCCC
EGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQ
CCCCEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
AALTQARELLAKH
HHHHHHHHHHHCC
>Mature Secondary Structure
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGAN
CEEEEEECCEEEEEHEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC
KAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQV
HHHCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCCCEEHHHHHHHH
GQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKENYQLIFDRYKSLRRQVIDKQK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCC
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEYQLLTGDDLSSGDLESELKALEKQLVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFTTSKFNRDGNESLEFYISTNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCEEEEEECCCC
EGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQ
CCCCEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
AALTQARELLAKH
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]