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The map label for this gene is ptsI [H]

Identifier: 21910582

GI number: 21910582

Start: 1087700

End: 1089424

Strand: Reverse

Name: ptsI [H]

Synonym: SpyM3_1046

Alternate gene names: 21910582

Gene position: 1089424-1087700 (Counterclockwise)

Preceding gene: 21910583

Following gene: 21910581

Centisome position: 57.32

GC content: 43.19

Gene sequence:

>1725_bases
ATGCTTAAAGGAATTGCAGCCTCAGACGGCGTTGCTGTTGCTAAAGCATATCTACTAGTTCAGCCGGATTTGTCATTTGA
GACTGTTACAGTCGCAGATACAAATGCAGAAGAAGCTCGCCTTGATGTTGCACTCCAAGCTGCACAAGACGAGCTTTCTG
TTATCCGTGAAAATGCAGTAGAAAGCTTAGGTGAAGAAGCAGCAGCCGTTTTTGATGCCCATTTGATGGTTCTTGCTGAT
CCAGAAATGATCAGCCAGGTTAAAGAAACGATTCGCGCAAAACAAACGAATGCAGAAACAGGTCTTAAAGAAGTGACTGA
CATGTTCATCACCATCTTTGAAGGCATGGAAGATAACCCATACATGCAAGAACGTGCAGCGGACATCCGCGACGTTGCAA
AGCGTGTGTTGGCTCACCTTTTAGGTGTAAAACTTCCAAATCCAGCTACAATCAATGAAGAATCAATCGTTATCGCACAC
GATTTGACACCTTCAGATACTGCTCAACTTAACAAACAATTTGTAAAAGCATTTGTTACAAATATCGGTGGTCGTACAAG
TCACTCAGCTATCATGGCACGTACACTTGAGATCGCTGCGGTACTTGGAACAAATGATATTACAAAACGTGTTAAAGATG
GTGATGTGATTGCCGTTAATGGTATCACTGGTGAAGTGATTATCGATCCAAGCGAAGATCAAGTACTTGCTTTTAAAGAA
GCTGGTGCGGCTTATGCCAAACAAAAAGCAGAGTGGTCTCTCCTTAAAGATGCGCACACTGAAACAGCTGATGGCAAACA
CTTTGAATTGGCTGCTAATATCGGTACGCCTAAAGACGTTGAAGGTGTTAATGACAATGGTGCTGAAGCTGTTGGCCTTT
ACCGTACTGAGTTCTTGTACATGGATTCTCAAGACTTCCCAACTGAAGACGAACAATACGAAGCTTACAAGGCAGTGCTT
GAAGGCATGAATGGCAAACCTGTTGTGGTTCGTACGATGGATATTGGTGGCGACAAGGAACTTCCTTACTTTGACCTTCC
AAAAGAAATGAATCCATTCCTTGGTTTCCGTGCTCTTCGTATTTCCATCTCTGAAACTGGGGATGCCATGTTCCGCACAC
AAATGCGTGCGCTTCTTCGTGCCTCTGTTCACGGACAACTTCGTATTATGTTCCCAATGGTTGCGCTTCTTAAAGAATTC
CGTGCTGCAAAAGCAGTCTTTGACGAAGAAAAAGCAAACTTGCTTGCAGAAGGCGTTGCGGTTGCTGATGACATCCAAGT
TGGTATCATGATTGAGATTCCTGCAGCTGCTATGCTTGCAGACCAATTTGCTAAGGAAGTTGATTTCTTCTCAATTGGAA
CAAACGACCTTATCCAATACACTATGGCAGCAGACCGTATGAACGAACAAGTATCATACCTTTACCAACCATACAACCCA
TCAATATTACGTTTGATCAACAATGTGATCAAAGCAGCGCACGCTGAAGGTAAATGGGCAGGTATGTGTGGTGAGATGGC
AGGTGACCAACAAGCTGTTCCACTTCTTGTCGGAATGGGCTTGGATGAGTTTTCTATGTCAGCAACTTCAGTACTTCGTA
CGCGTAGTTTAATGAAGAAACTTGACTCTGCTAAGATGGAAGAATATGCAAATCGTGCGCTTACAGAATGTTCAACAGCA
GAAGAAGTTCTTGAACTTTCTAAAGAATACGTTTCTGAAGATTAA

Upstream 100 bases:

>100_bases
GATGTTACTATCTCAGCTGAAGGTGCAGATGCTGAAGATGCCATTGCAGCAATTGAAGAAACAATGACTAAAGAGGGATT
GGCATAAGACTATGACAGAA

Downstream 100 bases:

>100_bases
CGGGTTAAAGAGTTGGTTTAAACCAGCTCTTTTTTGCTAGCTAAAAGTACAATTACTTTTTGGTGTGAAATCATTTGAGT
TATCAAATAATTATCGGAAT

Product: putative phosphoenolpyruvate:sugar phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLAD
PEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVIAH
DLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLAFKE
AGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVL
EGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLKEF
RAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNP
SILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTA
EEVLELSKEYVSED

Sequences:

>Translated_574_residues
MLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLAD
PEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVIAH
DLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLAFKE
AGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVL
EGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLKEF
RAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNP
SILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTA
EEVLELSKEYVSED
>Mature_574_residues
MLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLAD
PEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVIAH
DLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLAFKE
AGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVL
EGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLKEF
RAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNP
SILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTA
EEVLELSKEYVSED

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=45.9130434782609, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI48994992, Length=504, Percent_Identity=36.9047619047619, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1788726, Length=589, Percent_Identity=36.3327674023769, Blast_Score=328, Evalue=7e-91,
Organism=Escherichia coli, GI1789193, Length=573, Percent_Identity=32.6352530541012, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1787994, Length=417, Percent_Identity=29.9760191846523, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI226510935, Length=176, Percent_Identity=30.1136363636364, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 62775; Mature: 62775

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAV
CCCCCCCCCCHHEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNP
HHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
YMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVIAHDLTPSDTAQLNKQFVKAFVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
NIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLAFKE
HCCCCCCHHHHHHHHHHHHHEECCCHHHHHCCCCCEEEEECCCEEEEECCCCCCEEEEHH
AGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLY
HCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEE
MDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALR
ECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEEE
ISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLKEFRAAKAVFDEEKANLLAEGVA
EEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
VADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNP
EECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKK
HHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
LDSAKMEEYANRALTECSTAEEVLELSKEYVSED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAV
CCCCCCCCCCHHEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNP
HHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
YMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVIAHDLTPSDTAQLNKQFVKAFVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
NIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLAFKE
HCCCCCCHHHHHHHHHHHHHEECCCHHHHHCCCCCEEEEECCCEEEEECCCCCCEEEEHH
AGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLY
HCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEE
MDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALR
ECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEEE
ISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLKEFRAAKAVFDEEKANLLAEGVA
EEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
VADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNP
EECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKK
HHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
LDSAKMEEYANRALTECSTAEEVLELSKEYVSED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA