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The map label for this gene is rsuA [H]

Identifier: 21910554

GI number: 21910554

Start: 1056277

End: 1057008

Strand: Reverse

Name: rsuA [H]

Synonym: SpyM3_1018

Alternate gene names: 21910554

Gene position: 1057008-1056277 (Counterclockwise)

Preceding gene: 21910556

Following gene: 21910549

Centisome position: 55.62

GC content: 43.44

Gene sequence:

>732_bases
ATGCGTTTAGATAAATTTTTAGTGGCAACTGGTGTTGGAACTAGGAGCCAAGTCAAACTTCTCTTAAAAAAGAAGGCTAT
CTTCGTCAATCAAAAGGTTGAAACCTCTGCCAAAGCACACATTGATGAATACAAGGATTTGGTGACGTATCAAGGCACGC
CCTTGGTCTATGAATCCTTTGTTTATTACCTGTTAAACAAGCCGTCAGGGTATGTGTCGGCAACCCAGGACAGACAGCAG
GCTACTGTTATGGAGTTGTTAGATGACACGGCTAGGCAAAAAACAGTTTTTCCTGTGGGTAGGTTAGATAAGGATACCCG
TGGACTTTTGCTCTTGACCAATAATGGACAACTAGCCCATGACCTTTTATCCCCTAAAAAACATGTTACGAAAGAGTACT
TGGCTAAAGTAGCAGGCATCATGACTGAAGCTGATAAAGATTATTTTGCTAGAGGTATTTCCTTAAAGGATCACCAGTGC
CTACCTGCTCATTTAGAAGTGCTTGCTAGTGATTTACAGCAGCAAACAAGTTTGGTTAAAATTACCATACAAGAAGGAAA
ATTCCATCAGGTGAAACGCATGGTAGCAGCCTGTGGAAAGGAAGTGCTTGACTTGCAGCGTCTCAGCATGGGACCATTAA
AACTAGACCCTAGCCTTGCAGAAGGGGAGTTTAGGCGCCTGACGCCTGAGGAATTGCAGAGCCTGGCACCTTATTGTCAG
AGCTCACTATAG

Upstream 100 bases:

>100_bases
GTTTCTATAAGCCTATTATAATATATTAAGCGCTTACAGTCATGAAACCACTGTTTTTTTATGTTATACTTAATAATGAA
TTTTCTTTGAAAGGGTTATG

Downstream 100 bases:

>100_bases
AAAGAAACCTCCCCGGTCAGTACACTTGCTCAATAGTTGCGAAATGAGCATCATTGACAAGCTCTCTTTTTAATGTTATA
TAAAAGGCTTCCATGAGTGC

Product: putative 16S pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQ
ATVMELLDDTARQKTVFPVGRLDKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYCQ
SSL

Sequences:

>Translated_243_residues
MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQ
ATVMELLDDTARQKTVFPVGRLDKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYCQ
SSL
>Mature_243_residues
MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQ
ATVMELLDDTARQKTVFPVGRLDKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYCQ
SSL

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=37.3949579831933, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=30.8300395256917, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=39.041095890411, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27251; Mature: 27251

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESF
CCCHHEEHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VYYLLNKPSGYVSATQDRQQATVMELLDDTARQKTVFPVGRLDKDTRGLLLLTNNGQLAH
HHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCEEEEECCCCHHH
DLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQCLPAHLEVLASDLQQQTSLVK
HHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEE
ITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYCQ
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHH
SSL
CCC
>Mature Secondary Structure
MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESF
CCCHHEEHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VYYLLNKPSGYVSATQDRQQATVMELLDDTARQKTVFPVGRLDKDTRGLLLLTNNGQLAH
HHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCEEEEECCCCHHH
DLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQCLPAHLEVLASDLQQQTSLVK
HHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEE
ITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYCQ
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHH
SSL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]