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The map label for this gene is miaA [H]

Identifier: 21910170

GI number: 21910170

Start: 695327

End: 696226

Strand: Direct

Name: miaA [H]

Synonym: SpyM3_0634

Alternate gene names: 21910170

Gene position: 695327-696226 (Clockwise)

Preceding gene: 21910168

Following gene: 21910171

Centisome position: 36.59

GC content: 43.0

Gene sequence:

>900_bases
ATGACAAAAATAAAAATAGTTGTGATTGTTGGTCCGACAGCGGTTGGAAAAACAGCACTTGGGATTAGTTTAGCCAAGGC
TTTTAATGGAGAAATTATTTCAGGAGATAGCCAGCAAGTTTATCGACAGTTGGATATTGGAACAGCCAAGGCCACTCAAG
AAGAGCAAGAAGCAGCGGTTCATCACTTAATTGATATTCGTGAGGTGACGGAGTCTTATTCGGCCTACGATTTTGTTCAA
GATGCTCAAAAAGCTATTTCAGACATTGTTAGTCGTGGGAAATTACCAATTATTGTAGGTGGAACTGGTCTTTACTTACA
AAGTTTACTCGAAGGTTATCATCTAGGAGGTCAAGTTGATCAAGAGGCGGTAAAAGCCTATCGGAATGAGTTGGAGCAAT
TGGACGATCACGACTTATATGAACGCTTGCAAGTCAATAACATTACCATTGAGCAGGTGAATCGCAGGCGGGCGATCCGA
GCCCTTGAATTGGCTCAGTTTGCTGATGAATTAGAGAATGCCGAGACGGCTTATGAGCCGCTTATTATCGGCTTGAATGA
TGACCGCCAAGTCATCTATGACCGTATCAATCAGCGCGTGGATCGTATGCTTGAAAACGGCTTATTGGAAGAAGCAAAAT
GGTTATATGAGCATTACCCGACTGTTCAGGCTAGTCGTGGCATAGGTTATAAGGAATTATTCCCCTATTTTGTAGGTGAA
ATGACTTTGGCAGAAGCTAGTGATCAGTTAAAACAAAACACGAGACGTTTTGCCAAGCGACAGCTAACTTGGTTCCGCAA
TCGAATGGCTGTTAGCTTCACTGCCATAACGGCTCCTGATTACCCACAGGTAGTACATGATAGGGTCAGGGATTTTCTTG
GTCAGAAAGAGAAGTCATGA

Upstream 100 bases:

>100_bases
GGTCCTTAATCTTATGTTATAATATATGGTAGCGAAAAATAAGCTAATTTTCAAGTAAAAGGCGTTTTTGGACACGCTTT
TCTAATAGAAAGACAAGAAA

Downstream 100 bases:

>100_bases
CGCAAAATTAGCAGAATCTGTTGAAGATATGTTATAATAGAATTTACGGAATTTGGAGGATTGGTAAAGGTGATGATTGA
GACAAAGCGTCAGCAAGAAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQLDIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQ
DAQKAISDIVSRGKLPIIVGGTGLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEHYPTVQASRGIGYKELFPYFVGE
MTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAITAPDYPQVVHDRVRDFLGQKEKS

Sequences:

>Translated_299_residues
MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQLDIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQ
DAQKAISDIVSRGKLPIIVGGTGLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEHYPTVQASRGIGYKELFPYFVGE
MTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAITAPDYPQVVHDRVRDFLGQKEKS
>Mature_298_residues
TKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQLDIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQD
AQKAISDIVSRGKLPIIVGGTGLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIRA
LELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEHYPTVQASRGIGYKELFPYFVGEM
TLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAITAPDYPQVVHDRVRDFLGQKEKS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=330, Percent_Identity=32.4242424242424, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1790613, Length=291, Percent_Identity=33.6769759450172, Blast_Score=150, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=28.3333333333333, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=33.1034482758621, Blast_Score=149, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24649830, Length=341, Percent_Identity=31.9648093841642, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33994; Mature: 33863

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQLDIGTAKATQEEQEAAV
CCEEEEEEEECCCHHCHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCHHHHHHHHHHHH
HHLIDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGTGLYLQSLLEGYHLGGQVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCC
QEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIRALELAQFADELENAETAYEP
HHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
LIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEHYPTVQASRGIGYKELFPYFVGE
EEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAITAPDYPQVVHDRVRDFLGQKEKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQLDIGTAKATQEEQEAAV
CEEEEEEEECCCHHCHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCHHHHHHHHHHHH
HHLIDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGTGLYLQSLLEGYHLGGQVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCC
QEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIRALELAQFADELENAETAYEP
HHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
LIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEHYPTVQASRGIGYKELFPYFVGE
EEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAITAPDYPQVVHDRVRDFLGQKEKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]