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The map label for this gene is bcaT [H]

Identifier: 21910162

GI number: 21910162

Start: 688263

End: 689288

Strand: Direct

Name: bcaT [H]

Synonym: SpyM3_0626

Alternate gene names: 21910162

Gene position: 688263-689288 (Clockwise)

Preceding gene: 161485747

Following gene: 21910163

Centisome position: 36.21

GC content: 40.74

Gene sequence:

>1026_bases
ATGATGACAATAGCAATTGATTGGGACAACCTAGGATTTGAATATCATAAGTTGCCATTTCGTTACATTTCTTATTACAA
AAATGGTCAATGGGATAAAGGTCAATTAACAGAAGATGCCACCTTACATATCTCAGAAAGCGCTCCAGCGCTTCATTATG
GGCAACAAGCTTTTGAAGGTTTAAAGGCTTATCGCACCAAAGATGGCTCTATTCAACTATTTAGACCAGACCGTAATGCA
GTGCGTTTACAAGCCACAGCAGACCGCTTGTTGATGCCACAAGTATCAACAGAGCAATTTATTGATGCAGCTAAGCAAGT
GGTTAAAGCCAATGAAGACTTTGTACCACCTTACGGAACAGGTGCAACGCTTTACCTGAGACCGTTATTAATTGGAGTTG
GAGATATTATTGGGGTTAAACCAGCCGAAGAATATATTTTCACCATTTTTGCCATGCCAGTAGGAAACTATTTCAAAGGT
GGACTTGCTCCAACTAATTTTATTGTTTCTGAAGCGTTTGACCGCGCCGCTCCTTACGGAACAGGTGCTGCTAAGGTGGG
AGGAAACTATGCAGGAAGTTTGCTACCAGGTAAGGCCGCTAAATCAGCAGGCTTTTCTGATGTGATTTACTTAGATCCAG
CAACCCATACTAAAATTGAAGAAGTCGGAGCTGCAAACTTTTTTGGCATTACTGCAAATAATGAATTTGTTACGCCACTT
AGTCCATCCATCTTGCCATCTATTACGAAGTATTCACTCTTACAACTAGCTGAAGAAAGACTTGGTATGACAGTAATTGA
GGGTGATGTTCCAATTAATGAATTGGATAAATTTGTGGAAGTAGGAGCTTGTGGAACAGCAGCTGTTATCTCTCCAATTG
GCGGTATCCAGTACAAGGATAACTTACATGTCTTTTACAGTGAAACAGAAGTTGGTCCTGTGACACGCCGTTTATACGAT
GAGTTGGTCGGCATTCAATTTGGGGATATTGAAGCACCAGAAGGTTGGATTGTAAAAGTAGATTAA

Upstream 100 bases:

>100_bases
TAATTTTATGGTAAATTTAGAATATTAATGCAAAAGAGTAGAATAAACTTGAAAATTATTGAAAAAAAGTTACAATAGAA
AAAAGCAGAAAAGAGGTACG

Downstream 100 bases:

>100_bases
AATAGAGCTTGCTCTTGCAAGCTTTTTTGATTTTGTGTAAAATAGACAAAGTGAGGTAAAGTGATGAGTAAAAAAGATAA
AAAAATTGAAATCCAACTGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MMTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNA
VRLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKG
GLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPL
SPSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEVGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
ELVGIQFGDIEAPEGWIVKVD

Sequences:

>Translated_341_residues
MMTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNA
VRLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKG
GLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPL
SPSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEVGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
ELVGIQFGDIEAPEGWIVKVD
>Mature_341_residues
MMTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNA
VRLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKG
GLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPL
SPSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEVGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
ELVGIQFGDIEAPEGWIVKVD

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=334, Percent_Identity=32.9341317365269, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI296010904, Length=334, Percent_Identity=32.9341317365269, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI38176287, Length=334, Percent_Identity=32.9341317365269, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI50658084, Length=330, Percent_Identity=33.6363636363636, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI258614015, Length=300, Percent_Identity=35.3333333333333, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI296010902, Length=249, Percent_Identity=32.9317269076305, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI296010900, Length=249, Percent_Identity=32.9317269076305, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI48994963, Length=320, Percent_Identity=33.125, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=325, Percent_Identity=32.9230769230769, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17565728, Length=353, Percent_Identity=30.3116147308782, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322608, Length=319, Percent_Identity=33.5423197492163, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=317, Percent_Identity=31.8611987381703, Blast_Score=156, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24641779, Length=333, Percent_Identity=33.6336336336336, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37223; Mature: 37223

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEG
CEEEEEECCCCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
LKAYRTKDGSIQLFRPDRNAVRLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGT
HHHHCCCCCCEEEECCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYG
CCCEEEHHHHHHHHHHCCCCCHHHEEEEEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCC
TGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPL
CCHHHCCCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHCCCCEEEEEECCCCEECCC
SPSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEVGACGTAAVISPIGGIQYKD
CCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEC
NLHVFYSETEVGPVTRRLYDELVGIQFGDIEAPEGWIVKVD
CEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC
>Mature Secondary Structure
MMTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEG
CEEEEEECCCCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
LKAYRTKDGSIQLFRPDRNAVRLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGT
HHHHCCCCCCEEEECCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYG
CCCEEEHHHHHHHHHHCCCCCHHHEEEEEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCC
TGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPL
CCHHHCCCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHCCCCEEEEEECCCCEECCC
SPSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEVGACGTAAVISPIGGIQYKD
CCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEC
NLHVFYSETEVGPVTRRLYDELVGIQFGDIEAPEGWIVKVD
CEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]