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The map label for this gene is amiC [H]

Identifier: 21910154

GI number: 21910154

Start: 677510

End: 678964

Strand: Direct

Name: amiC [H]

Synonym: SpyM3_0618

Alternate gene names: 21910154

Gene position: 677510-678964 (Clockwise)

Preceding gene: 21910153

Following gene: 21910155

Centisome position: 35.65

GC content: 42.27

Gene sequence:

>1455_bases
ATGACTTATCAAGATGCGACGGCTATGGCTATTGCTGTTCAGACCGGTCAGACCACACCACTAGAATTAGTAACCCAGGC
TATTTATAAGGCTAAAAAGTTGAACCCTACCTTAAATGCTATTACCTCAGAACGCTTTGAAGCAGCCCTAGAAGAAGCCA
AACAAAGGGATTTTTCAGGTAAGCCTTTTGCAGGAGTTCCCATTTTTTTGAAAGATTTGGGACAAGAATTGAAGGGAGAC
TTATCAACATCGGGTTCTAAGCTATTCAAGAATCATCATGCGACTAAAACAGATTTATTCGTGAAAAGGTTAGAAGACTT
GGGATTTATTATTTTAGGGCGTTCTAACACACCTGAATTTGGCTTTAAAAATATTAGTGATAGTCAGCTCCATGGCCCGG
TTTCATTACCTCATGATAATACACGAAATGCTGGTGGCTCATCTGGGGGAGCTGCTGCTCTAGTGTCATCAGGAATTAGT
GCACTAGCAACTGCTAGTGATGGTGGTGGTTCTATTCGAATTCCAGCCTCGTTTAATGGCTTAATTGGGCTCAAACCTAG
CCGCGGGCGCATGCCTGTCGGACCTGGTTCTTATCGAAGCTGGCAAGGAGCCTCAGTTCATTTTGCCTTGACCAAATCCG
TTAGAGATACTAGAAATCTACTCTACTATTTACAGATGGAACAAATGGAAAGCCCCTTCCCCCTAGCCACCTTAACAAAA
AATAGTATTTATCAATCGCTACAACGTCCCTTGACAATTGCTTTTTATCAACGGTTAAGTGACGGTAGCCCAGTATCTTT
AGATACTGCAAAAGCTTTAAGACAAGCAGTAACTTGGTTAAGAGAACAAGGGCATCAGCTAGTTGAATTGGAAGAGTTTC
CTGTAAATATGACAGAAGTGATTCGTCATTATTATATTATGAATAGTGTGGAAACGGCTGCTATGTTTGCTGATATTGAA
GACACTTTTGGACGTCCGATGACCAAAGACGATATGGAAACCATGACCTGGGCTATTTACCAATCAGGTAAAGACATCCC
AGCTTGGCGTTACTCACAGGTCCTTCAAAAATGGGATACGTACAGTGCAACCATGGCTAGTTTTCATGAGACTTATGACC
TGTTGTTGACGTTCACAACCAACACACCAGCTCCTAAGCACGGAGAACTTGTCCCAGATTCGAAACTCATGGCTAACTTG
GCTCAAGCTGAAATTTTCAGCAGTGAAGAGCAATTCAATTTGGTCGAAACCATGTTTGGTAAGAGTCTGGCTATTAATCC
TTACACAGCTCTGCCTAATCTAACTGGCCAGCCAGCTATTAGTTTACCTACTTACGAAACTAAGGAAGGGCTCCCAATGG
GCATTCAACTAATTGCAGCCAAAGGTAGAGAGGACCTATTATTAGGAATTGCAGAGCAGTTTGAGGCAGCAGGATTATTG
AAGATTCCTCAATAA

Upstream 100 bases:

>100_bases
CACATTCTAATTTCTAACCTTTTAATGGTGGTTTTCTTTTATTTGTGTTTTAATAAAAAAGAATGTTTTAGGATTCATAT
CTTAGAAAGGAAGAGTAGCT

Downstream 100 bases:

>100_bases
TAATCTTATAAAACAGTTTAACTATTATAGATTAACTTTTTAAAGAGATTGAGCGCTCACTGATAACACAACTTAATCAA
AATGTTATACTATTCTTATC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGD
LSTSGSKLFKNHHATKTDLFVKRLEDLGFIILGRSNTPEFGFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGIS
ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
NSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIE
DTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANL
AQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
KIPQ

Sequences:

>Translated_484_residues
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGD
LSTSGSKLFKNHHATKTDLFVKRLEDLGFIILGRSNTPEFGFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGIS
ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
NSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIE
DTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANL
AQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
KIPQ
>Mature_483_residues
TYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGDL
STSGSKLFKNHHATKTDLFVKRLEDLGFIILGRSNTPEFGFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGISA
LATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTKN
SIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIED
TFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLA
QAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLLK
IPQ

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=510, Percent_Identity=26.6666666666667, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI222831590, Length=526, Percent_Identity=24.3346007604563, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=185, Percent_Identity=36.7567567567568, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=530, Percent_Identity=24.9056603773585, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=325, Percent_Identity=29.5384615384615, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=520, Percent_Identity=25.5769230769231, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=313, Percent_Identity=30.0319488817891, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=313, Percent_Identity=30.0319488817891, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=533, Percent_Identity=24.953095684803, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=156, Percent_Identity=35.8974358974359, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=420, Percent_Identity=24.5238095238095, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=428, Percent_Identity=24.7663551401869, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=198, Percent_Identity=30.3030303030303, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24652985, Length=512, Percent_Identity=26.7578125, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI19922090, Length=512, Percent_Identity=26.7578125, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24652981, Length=512, Percent_Identity=26.7578125, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24652983, Length=512, Percent_Identity=26.7578125, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24644968, Length=526, Percent_Identity=25.6653992395437, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45550774, Length=196, Percent_Identity=35.7142857142857, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648435, Length=196, Percent_Identity=35.7142857142857, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648437, Length=196, Percent_Identity=35.7142857142857, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=27.4853801169591, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=28.936170212766, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648441, Length=127, Percent_Identity=39.3700787401575, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=127, Percent_Identity=39.3700787401575, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 53122; Mature: 52991

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSG
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
KPFAGVPIFLKDLGQELKGDLSTSGSKLFKNHHATKTDLFVKRLEDLGFIILGRSNTPEF
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCC
GFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGISALATASDGGGSIRIPASFNG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHEEECCCCCCEEEECCCCCC
LIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
EEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHEEEEEHHHCCCCCCCHHHHH
NSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEV
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHH
IRHYYIMNSVETAAMFADIEDTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDT
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLAQAEIFSSEEQFNLVETMFG
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHC
KSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
CCEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCE
KIPQ
ECCC
>Mature Secondary Structure 
TYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSG
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
KPFAGVPIFLKDLGQELKGDLSTSGSKLFKNHHATKTDLFVKRLEDLGFIILGRSNTPEF
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCC
GFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGISALATASDGGGSIRIPASFNG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHEEECCCCCCEEEECCCCCC
LIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
EEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHEEEEEHHHCCCCCCCHHHHH
NSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEV
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHH
IRHYYIMNSVETAAMFADIEDTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDT
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLAQAEIFSSEEQFNLVETMFG
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHC
KSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
CCEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCE
KIPQ
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]