Click here to switch to the map view.

The map label for this gene is rmlD [H]

Identifier: 21910057

GI number: 21910057

Start: 575066

End: 575920

Strand: Direct

Name: rmlD [H]

Synonym: SpyM3_0521

Alternate gene names: 21910057

Gene position: 575066-575920 (Clockwise)

Preceding gene: 21910056

Following gene: 21910058

Centisome position: 30.26

GC content: 40.35

Gene sequence:

>855_bases
ATGATTTTAATTACAGGAAGCAATGGTCAATTAGGGACAGAACTTCGATACTTATTAGATGAACGAGGTGTTGATTATGT
AGCCGTTGATGTCGCAGAAATGGACATCACAAATGAAGACAAAGTCGAAGCTGTTTTTGCGCAGGTCAAGCCAACATTGG
TCTATCATTGTGCTGCTTATACGGCAGTTGATGCAGCAGAAGACGAAGGAAAAGCCTTGAATGAGGCTATTAATGTAACA
GGCTCAGAAAATATTGCCAAGGCTTGTGGAAAATATGGGGCTACCCTTGTTTACATTTCAACAGACTACGTATTTGATGG
CAATAAACCAGTCGGTCAAGAATGGGTAGAAACAGATCGTCCAGACCCTAAGACAGAATATGGCCGTACAAAACGTTTAG
GAGAACTAGCTGTTGAACGATACGCAGAGCATTTTTATATTATCCGTACTGCTTGGGTATTTGGAAATTATGGTAAAAAC
TTTGTCTTTACCATGGAACAGTTAGCGGAAAATCACTCCCGTTTGACTGTTGTCAATGACCAACACGGTCGGCCAACGTG
GACAAGAACCTTGGCAGAATTCATGTGTTATTTGACAGAGAATCAAAAAGCCTTTGGTTACTATCACTTGTCAAATGATG
CTAAGGAAGACACCACTTGGTATGATTTTGCCAAAGAAATCTTAAAAGACAAGGCTGTTGAAGTCGTGCCAGTTGATTCA
TCAGCTTTCCCAGCAAAAGCCAAACGTCCTTTAAATTCAACCATGAATCTTGATAAGGCAAAAGCAACAGGGTTTGTTAT
TCCAACATGGAAAGAAGCCTTGAAGGCATTTTACCAACAAGGACTGAAAAAGTAA

Upstream 100 bases:

>100_bases
ATGTAGAATAAAAGGAAATACAGGTGAAAGCCTTTGGTGAATGTTAGAGAACACTAAGATACTGAGAAAAGTGTTCTACC
TATGGCAAAAAGGAGAACTG

Downstream 100 bases:

>100_bases
GTATTCTAAAAAGAGTTGGCTTTCTAAGGCCAACTCTTTTTCATGGGTTTAAGGTCGCATTAGCCTTCTAATTGTGGTAT
AATATCACTGATTATATTTT

Product: putative dTDP-4-keto-L-rhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVT
GSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTWYDFAKEILKDKAVEVVPVDS
SAFPAKAKRPLNSTMNLDKAKATGFVIPTWKEALKAFYQQGLKK

Sequences:

>Translated_284_residues
MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVT
GSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTWYDFAKEILKDKAVEVVPVDS
SAFPAKAKRPLNSTMNLDKAKATGFVIPTWKEALKAFYQQGLKK
>Mature_284_residues
MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVT
GSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTWYDFAKEILKDKAVEVVPVDS
SAFPAKAKRPLNSTMNLDKAKATGFVIPTWKEALKAFYQQGLKK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=284, Percent_Identity=26.056338028169, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI11034825, Length=284, Percent_Identity=26.056338028169, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1788352, Length=291, Percent_Identity=36.0824742268041, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32008; Mature: 32008

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAY
CEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHH
TAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDR
HHHHCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCHHHHHCCC
PDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKNFVFTMEQLAENHSRLTVVND
CCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEHHHHHCCCCEEEEEEC
QHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTWYDFAKEILKDKAVEVVPVDS
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCC
SAFPAKAKRPLNSTMNLDKAKATGFVIPTWKEALKAFYQQGLKK
CCCCCHHCCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAY
CEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHH
TAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDR
HHHHCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCHHHHHCCC
PDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKNFVFTMEQLAENHSRLTVVND
CCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEHHHHHCCCCEEEEEEC
QHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTWYDFAKEILKDKAVEVVPVDS
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCC
SAFPAKAKRPLNSTMNLDKAKATGFVIPTWKEALKAFYQQGLKK
CCCCCHHCCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]