Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 21909836

GI number: 21909836

Start: 341275

End: 343272

Strand: Direct

Name: metG [H]

Synonym: SpyM3_0300

Alternate gene names: 21909836

Gene position: 341275-343272 (Clockwise)

Preceding gene: 21909835

Following gene: 21909837

Centisome position: 17.96

GC content: 44.19

Gene sequence:

>1998_bases
ATGAAAAAACCATTTTACATTACGACACCCATTTATTACCCATCTGGGAAATTACACATTGGTTCTGCCTATACAACTAT
TGCTTGTGATGTCTTGGCACGCTACAAGCGCCTGATGGGCCATGAGGTCTTTTACCTCACAGGACTAGATGAGCACGGAC
AAAAGATTCAAACCAAGGCAAAAGAAGCTGGTATTACACCTCAAACCTACGTTGATAACATGGCAAAGGATGTCAAAGCC
CTTTGGCAATTGCTAGATATTTCTTATGATAAATTTATCCGGACAACTGACGATTACCACGAAGAAGTCGTTGCTGCTGT
TTTTGAAAAATTGCTGGCGCAAGACGACATTTACCTTGGCGAATACTCTGGTTGGTATTCTGTCTCTGATGAGGAATTTT
TCACTGAAAGCCAATTAAAAGAAGTCTTTAGAGATGAAGACGGTCAGGTTATTGGAGGGATTGCCCCATCAGGTCATGAG
GTCGAGTGGGTCTCTGAAGAATCCTATTTCCTACGCCTTAGCAAATACGCTGATCGTCTTGTGGCCTTCTTTAAAGAGCG
ACCTGACTTTATCCAACCAGACGGTCGTATGAATGAAATGGTGAAAAACTTTATCGAGCCAGGCCTAGAAGATTTGGCTG
TTAGCCGTACTACCTTTACATGGGGTGTACCTGTTCCATCAGACCCAAAACACGTGGTTTATGTCTGGATTGATGCCCTC
TTAAACTACGCGACAGCTCTTGGTTATGGTCAAGCTAATCACGCTAACTTTGACAAATTCTGGAATGGAACGGTTTTCCA
CATGGTTGGAAAAGATATCTTACGTTTTCATAGCATCTACTGGCCAATCTTACTCATGATGCTTGATTTACCAATGCCAG
ACCGCTTAATTGCCCATGGTTGGTTTGTCATGAAAGACGGTAAAATGTCTAAATCAAAAGGCAATGTGGTCTATCCAGAA
ATGTTAGTGGAGCGCTTTGGACTGGATCCTCTTCGTTATTACCTCATGCGTTCTTTGCCAGTGGGTTCAGACGGGACCTT
TACCCCTGAAGATTATGTGGGTCGTATCAACTATGAATTGGCCAACGATCTTGGTAATTTATTGAATCGTACCGTTGCCA
TGATCAACAAGTATTTTGACGGCACGGTGCCAGCTTATGTTGACAATGGAACTGCCTTTGATGCTGACTTAAGTCAAGTG
ATTGATGCTCAGCTAGCAGACTACCATAAGCACATGGAAGCGGTGGACTACCCACGCGCCTTGGAGGCTGTTTGGACCAT
CATTGCTCGTACCAACAAATACATTGATGAGACAGCTCCTTGGGTGCTCGCTAAAGAAGATGGTGACAAGGCTCAACTAG
CCAGTGTCATGGCACACTTGGCAGCCAGCTTGCGCGTGGTCGCTCATGTGATCCAACCCTTTATGATGGAAACATCAGCT
GCTATCATGGCCCAGCTTGGCTTAGCGCCTGTTTCTGACTTGTCAACACTTGCTCTAGCAGATTTTCCTGCCAATACAAA
GGTCGTTGCCAAAGGCACACCGATTTTCCCACGTCTTGATATGGAAGCTGAGATTGACTACATCAAAGCTCAAATGGGTG
ACAGCTCAGCTATTTCCCAAGAAAAAGAATGGGTCCCTGAAGAGGTTGCGCTCAAATCTGAAAAAGACGTCATCACATTT
GAAACCTTTGATGCTGTGGAAATCCGTGTGGCAGAAGTCAAGGAAGTGTCAAAAGTGGAAGGTTCAGAAAAATTGCTGCG
CTTTAGAGTAGATGCAGGAGACGGGCAAGACCGTCAAATCTTGTCAGGTATTGCCAAATGCTACCCCAACGAACAAGAAT
TAGTGGGCAAAAAACTACAAATTGTTGCTAACCTGAAACCGCGTAAAATGATGAAAAAATACATCAGTCAAGGCATGATT
TTATCAGCAGAGCATGGAGATCAGCTAACGGTCTTAACCGTTGATTCCTCTGTTCCAAATGGAAGTATCATTGGCTGA

Upstream 100 bases:

>100_bases
ATTAGCCTTGAGGGTTGAATTTAAACAGGCAAAAACGAAATCCTACACTATGCATCTCAATCATACTTTCATTTGAGTAT
GGTTCTGAAAGGAAACTATG

Downstream 100 bases:

>100_bases
TGCTGACCTATTAATCAAAAAACCTAAGCAAGAACATGTGCTTAGGTTTTTTTGATTGCCTTCAAGAAAGACGAATAACG
AGATGGGTGATATGGCATCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKA
LWQLLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHE
VEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
LNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQV
IDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETSA
AIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITF
ETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKCYPNEQELVGKKLQIVANLKPRKMMKKYISQGMI
LSAEHGDQLTVLTVDSSVPNGSIIG

Sequences:

>Translated_665_residues
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKA
LWQLLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHE
VEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
LNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQV
IDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETSA
AIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITF
ETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKCYPNEQELVGKKLQIVANLKPRKMMKKYISQGMI
LSAEHGDQLTVLTVDSSVPNGSIIG
>Mature_665_residues
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKA
LWQLLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHE
VEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
LNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQV
IDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETSA
AIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITF
ETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKCYPNEQELVGKKLQIVANLKPRKMMKKYISQGMI
LSAEHGDQLTVLTVDSSVPNGSIIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=507, Percent_Identity=35.1084812623274, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI14043022, Length=587, Percent_Identity=27.427597955707, Blast_Score=189, Evalue=1e-47,
Organism=Escherichia coli, GI1788432, Length=696, Percent_Identity=28.0172413793103, Blast_Score=269, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI212645096, Length=496, Percent_Identity=31.4516129032258, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17540794, Length=571, Percent_Identity=26.6199649737303, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=29.9479166666667, Blast_Score=177, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321610, Length=484, Percent_Identity=32.6446280991736, Blast_Score=264, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=25.4901960784314, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21358483, Length=499, Percent_Identity=33.2665330661323, Blast_Score=262, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24655311, Length=566, Percent_Identity=24.5583038869258, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74885; Mature: 74885

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKA
CCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
KEAGITPQTYVDNMAKDVKALWQLLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLG
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
EYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYADRL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHH
VAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
HHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHHH
LNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHG
HHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
WFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYEL
EEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHHH
ANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQVIDAQLADYHKHMEAVDYPRA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
LEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETSA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQ
HHHHHHCCCCCCCHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHCH
EKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQI
HHHCCCHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCHHEEEEEECCCCCCHHHH
LSGIAKCYPNEQELVGKKLQIVANLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPN
HHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCC
GSIIG
CCCCC
>Mature Secondary Structure
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKA
CCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
KEAGITPQTYVDNMAKDVKALWQLLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLG
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
EYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYADRL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHH
VAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
HHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHHH
LNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHG
HHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
WFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYEL
EEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHHH
ANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQVIDAQLADYHKHMEAVDYPRA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
LEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETSA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQ
HHHHHHCCCCCCCHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHCH
EKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQI
HHHCCCHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCHHEEEEEECCCCCCHHHH
LSGIAKCYPNEQELVGKKLQIVANLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPN
HHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCC
GSIIG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]