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The map label for this gene is rluB [H]

Identifier: 21909805

GI number: 21909805

Start: 307852

End: 308574

Strand: Direct

Name: rluB [H]

Synonym: SpyM3_0269

Alternate gene names: 21909805

Gene position: 307852-308574 (Clockwise)

Preceding gene: 21909804

Following gene: 21909806

Centisome position: 16.2

GC content: 39.42

Gene sequence:

>723_bases
ATGCGAATTAACAAATACATTGCCCATGCGGGTATTGCCAGTCGACGTAAGGCTGAAGAATTGATTAAGCAAGGCTTAGT
AACCTTAAATGGTCAGGTTATTACGGATTTAGCAACCACTGTAAAATCGGGAGACGTGGTTGAAATAGAAGGAAGTCCTA
TTTACAACGAGGAAAAGGTTTACTATTTGCTTAATAAACCTCGTGGCGCTATCTCAAGTGTGTCAGATGACAAAGGCCGT
AAAACGGTACTGGATTTACTACCTCAGGTTAAAGAACGCATATACCCTGTTGGACGATTGGATTGGGACACATCAGGGGT
CCTTATTTTAACGAATGATGGTGATTTTACAGATACTATGATTCATCCTAGAAATGAAATAGACAAGGTTTATCTGGCGC
GTGTCAAAGGAATTGCAACGAAAGAAAATCTTCGTCCCTTAACTCGAGGAATTGTTATTGATGGTAAAAAGACCAAACCA
GCTCGCTACAATATTGTTCGTGTAGAAGCTGATAAGAATCGATCGATCGTAGAATTGACTATCCATGAAGGGCGAAACCA
TCAAGTGAAAAAAATGTTTGAGTCTGTGGGGCTTTTGGTTGATAAATTATCTCGTACACGTTTTGGGACCGTCGACTTAA
AGGGACTAAGACCAGGAGAAGCAAGACGCTTAAATAAAAAAGAAATAAGTCAACTGCATAACTTAGCTAATACGAAAAAA
TGA

Upstream 100 bases:

>100_bases
AGCAAATGACGTTTACAAAAGGATTTGTTTTAAGGCGTTTTAGTTGAAAAAGCCTTAAACTAAGAACAACAAAAAGTTGC
TCAAAAGAAAGAAGAATATA

Downstream 100 bases:

>100_bases
TGAAAAAACTATTAATAGTATCTGTTAAAGCTTATCAAAAATACATTTCTCCTTTATCTCCTCCTAGTTGTCGCTACAAG
CCAACCTGTTCAGCTTATAT

Product: putative ribosomal large subunit pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGR
KTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQLHNLANTKK

Sequences:

>Translated_240_residues
MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGR
KTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQLHNLANTKK
>Mature_240_residues
MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGR
KTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQLHNLANTKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=36.9747899159664, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=33.7552742616034, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=32.3770491803279, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081838, Length=173, Percent_Identity=32.3699421965318, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27035; Mature: 27035

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKV
CCCHHHHHHCCHHHHHHHHHHHHCCCEEECCEEHHHHHHHHCCCCEEEECCCCCCCCCEE
YYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTM
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCE
IHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKPARYNIVRVEADKNRSIVELT
ECCCHHHHHHHHHHHHCCCCHHHCCHHHCCEEEECCCCCCCEEEEEEEEECCCCCEEEEE
IHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQLHNLANTKK
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKV
CCCHHHHHHCCHHHHHHHHHHHHCCCEEECCEEHHHHHHHHCCCCEEEECCCCCCCCCEE
YYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTM
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCE
IHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKPARYNIVRVEADKNRSIVELT
ECCCHHHHHHHHHHHHCCCCHHHCCHHHCCEEEECCCCCCCEEEEEEEEECCCCCEEEEE
IHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQLHNLANTKK
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]