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The map label for this gene is ysgA [H]

Identifier: 21909794

GI number: 21909794

Start: 300424

End: 301161

Strand: Direct

Name: ysgA [H]

Synonym: SpyM3_0258

Alternate gene names: 21909794

Gene position: 300424-301161 (Clockwise)

Preceding gene: 21909791

Following gene: 21909795

Centisome position: 15.81

GC content: 42.14

Gene sequence:

>738_bases
ATGGTTATTACATCAAAAGCAAATCAACTAATTAAGCAAACAAAAAAATTATTGCAAAAAAAGCATCGAAAACAGTCCTA
TTTGATTGAGGGCTGGCACCTTTTTGAAGAAGCCCAAAAATCAGGACAAGTTTTTCGCCACATCTTTGTTTTAGAGGAAA
TGGCGGAGCGTCTAGCAGGTGAGCAAGAACTCGTTATTGTTAGCCCACAAGTGCTCAAGGAGTTGACCGACTCTCCTAGT
CCACAAGGCATTGTTGCTGAGGTGGAGATACCTAAGTTAGCCTTTCCATCAGATTACAAAGGGAAATATTTGGTTTTAGA
GGACGTCCAAGACCCAGGAAATCTTGGGACCATCATTCGTACAGCAGACGCTGCACGGTTTGATGGGGTCTTTCTTTCTG
AAAAATCAGCAGATATTTACAACCAAAAAACCCTACGTTCCATGCAAGGTAGCCATTTTCATTTACCAATTTGGCGAACA
GATGTCTATCAGCTTTGTCGAGAGCTCCAAGAGTATAAAACTCCCATCCTAGCAACAACTCTGTCAAAGAAATCTGTTGA
TTATAAAAGCCTCACACATCATGAAAGGTTGGCTTTGGTGTTGGGAAATGAAGGCCAGGGCATTAGTGCTGAAATGGCAG
CCCTAGCAGACCAACTGGTTCATATTACAATGCCTGGTCAGGCGGAAAGTTTGAATGTTGCCGTAGCAGCAGGAATCCTC
ATTTTCAGCTTGATTTAA

Upstream 100 bases:

>100_bases
CAAAAAAGGTCTTTTAGGTTTAAAACATTGAACTGCCCCCAAATTGACCATCTAAGAAGTTGATGCTTTCAAATGCTATC
GCGTATGGTATAATAGGCGC

Downstream 100 bases:

>100_bases
GCTAATATGATATAATAACCAAGTGAGGTAATTATGTTATGCATTATACAGAAGATAAAGAATATATGGAACACGTCGGT
CATTTGATCGCTCATCCTCG

Product: 23S rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQVFRHIFVLEEMAERLAGEQELVIVSPQVLKELTDSPS
PQGIVAEVEIPKLAFPSDYKGKYLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQLVHITMPGQAESLNVAVAAGIL
IFSLI

Sequences:

>Translated_245_residues
MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQVFRHIFVLEEMAERLAGEQELVIVSPQVLKELTDSPS
PQGIVAEVEIPKLAFPSDYKGKYLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQLVHITMPGQAESLNVAVAAGIL
IFSLI
>Mature_245_residues
MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQVFRHIFVLEEMAERLAGEQELVIVSPQVLKELTDSPS
PQGIVAEVEIPKLAFPSDYKGKYLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQLVHITMPGQAESLNVAVAAGIL
IFSLI

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=286, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790623, Length=240, Percent_Identity=30, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790083, Length=139, Percent_Identity=30.9352517985612, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27424; Mature: 27424

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQVFRHIFVLEEMAERLAG
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGKYLVLEDVQDPGNLGTIIR
CCEEEEECHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEE
TADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRTDVYQLCRELQEYKTPILATT
ECCCHHHCCEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEE
LSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQLVHITMPGQAESLNVAVAAGIL
HHHCCCCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
IFSLI
HHHCC
>Mature Secondary Structure
MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQVFRHIFVLEEMAERLAG
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGKYLVLEDVQDPGNLGTIIR
CCEEEEECHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEE
TADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRTDVYQLCRELQEYKTPILATT
ECCCHHHCCEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEE
LSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQLVHITMPGQAESLNVAVAAGIL
HHHCCCCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
IFSLI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]