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The map label for this gene is pepA [H]

Identifier: 21909625

GI number: 21909625

Start: 109560

End: 110627

Strand: Reverse

Name: pepA [H]

Synonym: SpyM3_0089

Alternate gene names: 21909625

Gene position: 110627-109560 (Counterclockwise)

Preceding gene: 21909630

Following gene: 21909624

Centisome position: 5.82

GC content: 45.51

Gene sequence:

>1068_bases
ATGACAGACTTATTCTCAAAAATCAAAGAAGTTACCGAACTGGATGGCATTGCGGGCTATGAACATAGCGTTCGTGACTA
CCTACGCACCAAAATAACCCCGCTGGTTGACCGTGTTGAAACAGACGGGCTTGGTGGCATTTTTGGTATCAGAGATAGTA
AAGCTGAAAAAGCCCCCCGTATTTTAGTAGCTGCGCACATGGACGAAGTCGGTTTTATGGTCAGTGATATCAAAGTTGAC
GGAACGCTACGCGTGGTTGGTATCGGTGGTTGGAACCCACTTGTTGTCAGTTCACAACGGTTTACCCTTTACACACGCAC
TGGCCAAGTTATTCCCCTTATTTCAGGATCGGTACCTCCTCATTTTCTCCGTGGGGCAAATGGCTCTGCTAGTCTACCAC
ATATCGAAGATATTGTGTTTGATGGTGGCTTTACGGATAAGGCAGAAGCTGAAAGATTTGGTATTACACCGGGTGATATT
ATTGTCCCTCAATCTGAAACGATCCTAACAGCCAATCAAAAAAATATTATTTCAAAAGCTTGGGACAATCGCTATGGTGT
TCTCATGATAACAGAAATGCTTGAAGCGTTAAAAGGACAAGACCTTAACAACACCCTAATTGCAGGTGCTAACGTTCAAG
AAGAAGTTGGTCTGCGCGGAGCCCACGTCTCAACCACCAAGTTCGACCCTGAACTCTTTTTCGCAGTAGATTGTTCGCCT
GCTGGTGATATTTATGGCAATCCTGGAACAATCGGAGATGGCACCTTGTTGCGTTTCTACGACCCAGGCCATGTCATGCT
CAAAGATATGCGCGACTTCTTACTGACTACTGCTGAGGACGCTGGTGTCAATTTCCAATACTATTGTGGCAAGGGAGGCA
CAGATGCAGGTGCTGCACACCTTCAAAATGGTGGTGTCCCATCAACAACCATCGGAGTCTGTGCACGCTACATTCACTCT
CATCAAACCCTCTACGCTATGGATGATTTCGTAGAAGCCCAAGCCTTCTTACAAGCCATTATCAAAAAACTGGATCGCTC
AACCGTTGACTTGATTAAATGTTACTAA

Upstream 100 bases:

>100_bases
TTTTTCTTTGCTGTGAAACCCATTCTTACTTAAAAAGTGGTAGAATATAGGGAGATGACCGCGTTGAACTGGTCTGAAAC
CTTGTAAATGGAGATGAAAA

Downstream 100 bases:

>100_bases
ACTAGCATTAGCCTGATTAACCATAATATCCCACAAGGAGCAACAGAATGAAAATTGGCATTATTGGTGTTGGCAAAATG
GCTAGCGCTATCATCAAAGG

Product: putative glutamyl-aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGLGGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVD
GTLRVVGIGGWNPLVVSSQRFTLYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDI
IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGLRGAHVSTTKFDPELFFAVDCSP
AGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLTTAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHS
HQTLYAMDDFVEAQAFLQAIIKKLDRSTVDLIKCY

Sequences:

>Translated_355_residues
MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGLGGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVD
GTLRVVGIGGWNPLVVSSQRFTLYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDI
IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGLRGAHVSTTKFDPELFFAVDCSP
AGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLTTAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHS
HQTLYAMDDFVEAQAFLQAIIKKLDRSTVDLIKCY
>Mature_354_residues
TDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGLGGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDG
TLRVVGIGGWNPLVVSSQRFTLYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDII
VPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGLRGAHVSTTKFDPELFFAVDCSPA
GDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLTTAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSH
QTLYAMDDFVEAQAFLQAIIKKLDRSTVDLIKCY

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=349, Percent_Identity=30.0859598853868, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788727, Length=351, Percent_Identity=30.4843304843305, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082417, Length=354, Percent_Identity=27.4011299435028, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017538
- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: =3.4.11.7 [H]

Molecular weight: Translated: 38551; Mature: 38420

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGLGGIFGIRDSKAEKAPR
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCE
ILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFTLYTRTGQVIPLISGSVPP
EEEEECCCHHCEEEEEEEECCEEEEEEECCCCCEEEECCEEEEEEECCCEEEEECCCCCH
HFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDIIVPQSETILTANQKNIISKA
HHHCCCCCCCCCCCHHHEEECCCCCCCCCHHHCCCCCCCEECCCCCEEEECCHHHHHHHH
WDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGLRGAHVSTTKFDPELFFAVDCSP
HCCCCCEEEHHHHHHHHCCCCCCCEEEECCCCHHHHCCCCCEEECCCCCCEEEEEEECCC
AGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLTTAEDAGVNFQYYCGKGGTDAGAAH
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH
LQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQAFLQAIIKKLDRSTVDLIKCY
HCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHEECC
>Mature Secondary Structure 
TDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGLGGIFGIRDSKAEKAPR
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCE
ILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFTLYTRTGQVIPLISGSVPP
EEEEECCCHHCEEEEEEEECCEEEEEEECCCCCEEEECCEEEEEEECCCEEEEECCCCCH
HFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDIIVPQSETILTANQKNIISKA
HHHCCCCCCCCCCCHHHEEECCCCCCCCCHHHCCCCCCCEECCCCCEEEECCHHHHHHHH
WDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGLRGAHVSTTKFDPELFFAVDCSP
HCCCCCEEEHHHHHHHHCCCCCCCEEEECCCCHHHHCCCCCEEECCCCCCEEEEEEECCC
AGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLTTAEDAGVNFQYYCGKGGTDAGAAH
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH
LQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQAFLQAIIKKLDRSTVDLIKCY
HCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8535515 [H]