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The map label for this gene is tyrS [H]

Identifier: 21909609

GI number: 21909609

Start: 90428

End: 91684

Strand: Reverse

Name: tyrS [H]

Synonym: SpyM3_0073

Alternate gene names: 21909609

Gene position: 91684-90428 (Counterclockwise)

Preceding gene: 21909624

Following gene: 21909608

Centisome position: 4.82

GC content: 41.61

Gene sequence:

>1257_bases
ATGAATATTTTTGAAGAACTCAAAGCTCGTGGCTTGGTCTTTCAAACAACTGATGAACAAGCCCTTGTCAAAGCATTAAC
AGAAGGGCAAGTATCCTATTATACCGGTTATGATCCAACCGCTGACAGCCTTCATCTGGGCCATCTAGTGGCTATCTTAA
CATCTCGTCGCTTGCAACTAGCAGGGCACAAACCTTACGCTCTTGTGGGTGGTGCCACAGGTTTAATTGGTGATCCTTCC
TTTAAAGATGCAGAGCGCAGCCTTCAAACCAAAGAAACAGTTTTGGAGTGGAGTGACAAGATTAAAGGGCAGTTGTCTAC
TTTCCTTGATTTTGAAAATGGTGATAATAAAGCAGAGCTTGTCAACAACTACGACTGGTTCTCGCAAATCAGCTTTATTG
ACTTCCTCCGTGATGTCGGTAAATACTTCACCGTTAACTACATGATGAGTAAAGACTCTGTTAAAAAACGCATTGAAACA
GGCATTTCTTACACTGAGTTTGCTTACCAAATCATGCAGGGTTACGACTTCTACGAACTCAATGACAAGCATAATGTAAC
CTTACAAATTGGCGGCTCTGACCAGTGGGGCAATATGACAGCTGGTACTGAATTGCTCCGCAAAAAGGCTGATAAAACTG
GGCACGTCATGACTGTACCACTCATCACTGACTCAACCGGGAAAAAATTCGGTAAATCAGAAGGTAATGCTGTCTGGCTT
GATGCCGATAAGACGTCTCCTTACGAGATGTACCAATTCTGGTTAAATGTCATGGATGATGATGCAGTGCGTTTCTTGAA
AATCTTCACCTTCTTATCTCTAGATGAGATTGCAGAAATTGAAAGTCAATTTAATGCCGCTCGTCACGAGCGTCTCGCTC
AAAAAACCTTGGCACGCGAAGTAGTTACCTTGGTTCATGGCGAAGAAGCTTATAAACAAGCTCTTAACATTACCGAGCAG
TTATTTGCTGGAAACATCAAAAATCTTTCTGCTAATGAATTAAAACAAGGATTGAGCAATGTACCAAATTATCACGTTCA
ATCAGAAGACAGCCTTAACCTTGTTGACATGCTTGTCACCGCAGGTATTTCCCCATCAAAACGCCAAGCGCGTGAAGACG
TGCAAAATGGAGCAATCTACATTAACGGAGACCGCATTCAAGATTTAGATTACCAATTAAGTAATGATGATAAAATTGAT
GATCAATTAACCGTTATTCGCCGCGGTAAGAAAAAATACGCTGTTCTCACTTACTAG

Upstream 100 bases:

>100_bases
ATACCTCATTTATCATTATACCAAACTTTGATAAAGCTTGCATAGCAGTAGCGTTTTTTGATATAATGATTCGAAACTAT
AATGAAAATGGAGAAAGCCT

Downstream 100 bases:

>100_bases
ATAACCGATTAAACGAGCTGAGCTCTGGCTCGTTTTTTCATAAGGAGATGTACTTATGACAGAATGTATCTTTTGCCATC
AGCTCAAAGCAAATCAGCTA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIESQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQ
LFAGNIKNLSANELKQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRIQDLDYQLSNDDKID
DQLTVIRRGKKKYAVLTY

Sequences:

>Translated_418_residues
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIESQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQ
LFAGNIKNLSANELKQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRIQDLDYQLSNDDKID
DQLTVIRRGKKKYAVLTY
>Mature_418_residues
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIESQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQ
LFAGNIKNLSANELKQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRIQDLDYQLSNDDKID
DQLTVIRRGKKKYAVLTY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=432, Percent_Identity=35.8796296296296, Blast_Score=236, Evalue=4e-62,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=50.5938242280285, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25145802, Length=400, Percent_Identity=33.75, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI25145805, Length=336, Percent_Identity=31.8452380952381, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325160, Length=442, Percent_Identity=34.841628959276, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=38.3908045977011, Blast_Score=305, Evalue=4e-83,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47270; Mature: 47270

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQL
CCHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHEE
AGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAEL
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHH
VNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIETGISYTEFAYQIMQGYDFYEL
HCCCHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHCCCHHHHHHHHHHCCCEEEE
NDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
CCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIESQFNAARHERLAQKTLARE
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTLVHGEEAYKQALNITEQLFAGNIKNLSANELKQGLSNVPNYHVQSEDSLNLVDMLVT
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHH
AGISPSKRQAREDVQNGAIYINGDRIQDLDYQLSNDDKIDDQLTVIRRGKKKYAVLTY
CCCCCHHHHHHHHHHCCEEEEECCEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQL
CCHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHEE
AGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAEL
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHH
VNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIETGISYTEFAYQIMQGYDFYEL
HCCCHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHCCCHHHHHHHHHHCCCEEEE
NDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
CCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIESQFNAARHERLAQKTLARE
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTLVHGEEAYKQALNITEQLFAGNIKNLSANELKQGLSNVPNYHVQSEDSLNLVDMLVT
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHH
AGISPSKRQAREDVQNGAIYINGDRIQDLDYQLSNDDKIDDQLTVIRRGKKKYAVLTY
CCCCCHHHHHHHHHHCCEEEEECCEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA