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The map label for this gene is purK [H]

Identifier: 21909564

GI number: 21909564

Start: 49302

End: 50321

Strand: Direct

Name: purK [H]

Synonym: SpyM3_0028

Alternate gene names: 21909564

Gene position: 49302-50321 (Clockwise)

Preceding gene: 21909563

Following gene: 21909565

Centisome position: 2.59

GC content: 50.29

Gene sequence:

>1020_bases
ATGGCCATTGCGGCTATCTATATGGGCCACAAGGTCATCACGCTGGATCCTGCTAGCGACTGCCCTGCCTCCCGCGTTAG
CGAGGTAATTGTGGCGCCTTACGATGATGTTGAGGCTTTGGGACAATTGGCTGCGCGTTGCGATGTATTGACTTATGAGT
TTGAGAATGTTGATGCGGACGGTTTGGATGCCGTTGTGTCAGCTTGTCAGCTACCGCAGGGGACCGATCTGCTCCGCATT
TCCCAAAACCGTATCGTTGAAAAAGACTTTCTGGCAAATAAGGCTGGTGTGACAGTCGCTCCCTATAAGGTGGTGACGTC
TAGCCTTGACCTAGGGGGGCTTGATTTGACCAAGACCTATGTCCTCAAGACAGCGACAGGTGGTTATGACGGTCATGGGC
AAAAGATTATCCGCTCAGCAGAAGATTTGCCAGAGGCGCAGCAATTAGCTAACTCAGCTCAGTGTGTCTTGGAAGAGTTT
GTCAATTTCGACCTTGAAATCTCAGTTATCGTGTCTGGAAACGGAGAGGATGTGACGGTCTTTCCCGTTCAGGAAAATAT
CCACCGCAACAATATCCTGTCAAAAACCATCGTACCAGCTCGCATCTCAGATCAACTGGCTGATAAGGCTAAGAAAACGG
CGGTGCAGATTGCCAAGAAACTCCAGCTGTCAGGAACTCTCTGTGTGGAAATGTTTGCGACCGCAGATGATATCATTGTC
AATGAAATTGCCCCACGTCCCCACAACTCAGGGCACTACTCAATCGAGGCTTGCGACTTTTCACAGTTTGACACCCACAT
TTTGGGCGTGCTAGGAGCGCCGCTCCCAACTATCCACCTCCATGCTCCAGCCGTCATGCTCAATGTCCTCGGACAACACG
TCCAGCAGGCGATTGACTATGTGACTGAAAACCCTAGCGCCCACCTCCACATGTATGGTAAACTAGAAGCGAAACATAAC
CGCAAAATGGGACATGTGACACTATTTGCAGAAGATGCGGATGAGGTGGAGGAGTTTTAG

Upstream 100 bases:

>100_bases
GCTCATTTCCATGAGGAACAAGGAAAAATCGCAGAGGAGTCAAGTGGTGAACTCATTTAAGACCATTGGGATTATCGGTG
GTGGTCAGCTGGGGCAGATG

Downstream 100 bases:

>100_bases
AACAGGAGTAGATTGGTAAGATGGAGTTAAGGGAATTTAAAGCGTGGTTATTAAAAAACCATTTTGAAGAAGATGAGTTA
TACAAGGAATCTATAAAGTG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAIAAIYMGHKVITLDPASDCPASRVSEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRI
SQNRIVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAEDLPEAQQLANSAQCVLEEF
VNFDLEISVIVSGNGEDVTVFPVQENIHRNNILSKTIVPARISDQLADKAKKTAVQIAKKLQLSGTLCVEMFATADDIIV
NEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLEAKHN
RKMGHVTLFAEDADEVEEF

Sequences:

>Translated_339_residues
MAIAAIYMGHKVITLDPASDCPASRVSEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRI
SQNRIVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAEDLPEAQQLANSAQCVLEEF
VNFDLEISVIVSGNGEDVTVFPVQENIHRNNILSKTIVPARISDQLADKAKKTAVQIAKKLQLSGTLCVEMFATADDIIV
NEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLEAKHN
RKMGHVTLFAEDADEVEEF
>Mature_338_residues
AIAAIYMGHKVITLDPASDCPASRVSEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRIS
QNRIVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAEDLPEAQQLANSAQCVLEEFV
NFDLEISVIVSGNGEDVTVFPVQENIHRNNILSKTIVPARISDQLADKAKKTAVQIAKKLQLSGTLCVEMFATADDIIVN
EIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLEAKHNR
KMGHVTLFAEDADEVEEF

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=266, Percent_Identity=30.4511278195489, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788155, Length=204, Percent_Identity=27.9411764705882, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=361, Percent_Identity=33.5180055401662, Blast_Score=170, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006140
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C; PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 36740; Mature: 36609

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAAIYMGHKVITLDPASDCPASRVSEVIVAPYDDVEALGQLAARCDVLTYEFENVDAD
CEEEEEEECCEEEEECCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHCEEEEEECCCCCC
GLDAVVSACQLPQGTDLLRISQNRIVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTY
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCCCEECCHHHHHCCCCCCCCCEEEEE
VLKTATGGYDGHGQKIIRSAEDLPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTV
EEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEE
FPVQENIHRNNILSKTIVPARISDQLADKAKKTAVQIAKKLQLSGTLCVEMFATADDIIV
EECHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHH
NEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDY
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHH
VTENPSAHLHMYGKLEAKHNRKMGHVTLFAEDADEVEEF
HHCCCCCEEEEEEEEECCCCCCCCEEEEEECCHHHHHCC
>Mature Secondary Structure 
AIAAIYMGHKVITLDPASDCPASRVSEVIVAPYDDVEALGQLAARCDVLTYEFENVDAD
EEEEEEECCEEEEECCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHCEEEEEECCCCCC
GLDAVVSACQLPQGTDLLRISQNRIVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTY
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCCCEECCHHHHHCCCCCCCCCEEEEE
VLKTATGGYDGHGQKIIRSAEDLPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTV
EEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEE
FPVQENIHRNNILSKTIVPARISDQLADKAKKTAVQIAKKLQLSGTLCVEMFATADDIIV
EECHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHH
NEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDY
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHH
VTENPSAHLHMYGKLEAKHNRKMGHVTLFAEDADEVEEF
HHCCCCCEEEEEEEEECCCCCCCCEEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA