Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 21909562

GI number: 21909562

Start: 47218

End: 48492

Strand: Direct

Name: purD [H]

Synonym: SpyM3_0026

Alternate gene names: 21909562

Gene position: 47218-48492 (Clockwise)

Preceding gene: 21909560

Following gene: 21909563

Centisome position: 2.48

GC content: 48.78

Gene sequence:

>1275_bases
GTGGATGTTTTGAAATTACTTGTTGTTGGTTCAGGTGGGCGTGAACATGCGATTGCTAAGAAGTTGTTAGCGTCTAAGGG
TGTGGATCAGGTTTTTGTGGCACCTGGTAATGATGGTATGACCTTGGATGGTCTGGACTTGGTGAATATAGTAGTTTCCG
AACATTCTAGGTTGATTGCCTTTGCTAAGGAGAATGAGATTTCTTGGGCCTTTATTGGTCCTGATGATGCGCTAGCAGCT
GGTATCGTTGATGATTTCAACACTGCTGGACTCAGAGCTTTTGGTCCAACAAAGGCAGCAGCGGAGCTAGAGTGGTCAAA
AGACTTTGCCAAGGAAATCATGGTCAAATACAATGTCCCAACAGCAGCCTATGGCACATTTTCAGACTTCGAAAAAGCCA
AAGCCTACATCGAAGAGCAGGGCGCACCTATCGTGGTCAAGGCTGATGGCTTGGCGCTAGGTAAGGGTGTGGTCGTGGCT
GAAACCGTTGAGCAGGCGGTAGAGGCGGCGCAAGAGATGCTTCTGGACAATAAGTTTGGCGACTCAGGAGCGCGTGTGGT
TATCGAGGAATTCTTGGATGGCGAGGAGTTTTCCCTCTTTGCCTTTGTTAATGGTGATAAGTTCTACATCATGCCGACAG
CTCAGGATCACAAGCGTGCCTTTGACGGAGACAAGGGGCCAAACACTGGTGGTATGGGTGCCTATGCGCCAGTTCCTCAT
CTACCTCAGAGCGTGGTAGACACAGCGGTTGAGATGATCGTTAGGCCTGTCCTTGAGGGCATGGTTGCCGAAGGGCGTCC
TTATCTAGGTGTCCTCTATGTGGGGCTTATCCTGACAGCTGATGGACCTAAGGTTATCGAGTTCAACTCACGTTTCGGTG
ACCCTGAAACTCAGATTATCCTCCCTCGCCTGACTTCTGATTTCGCTCAGAATATTGACGATATCATGATGGGCATTGAG
CCTTACATCACTTGGCAGAAGGACGGTGTGACTTTGGGTGTTGTCGTTGCCTCAGAAGGCTATCCATTCGATTATGAGAA
AGGTGTGCCACTGCCTGAAAAGACCGACGGCGATATCATCACCTACTACGCAGGAGTTAAGTTTTCGGAAAATAGCGAGC
TACTTCTCTCAAACGGAGGACGTGTCTATATGCTTGTCACCACAGAAGACAGCGTCAAAGCAGGGCAGGACAAAATCTAT
ACCCAGCTAGCCCAACAAGACCCAACAGGTCTCTTCTACCGAAATGACATCGGAAGCAAAGCTATAAGAGAATAA

Upstream 100 bases:

>100_bases
CTACTATCACATTTTTACAATGTTTCAATTAGTGATAATTAATACATTGTATCAAAAGGCGAACATTTATGATATAATAT
CTAATTATAATACGAAAGAG

Downstream 100 bases:

>100_bases
GACTAACCATTCAAATATTAGCGTCAGCAATCAACGACCGAAGGTCGCTCCAAAAAGAAAGAAGAAAGGAACTGAACACG
GGACTAATTTCCTAGGAAAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVSEHSRLIAFAKENEISWAFIGPDDALAA
GIVDDFNTAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVA
ETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAFDGDKGPNTGGMGAYAPVPH
LPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIE
PYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSELLLSNGGRVYMLVTTEDSVKAGQDKIY
TQLAQQDPTGLFYRNDIGSKAIRE

Sequences:

>Translated_424_residues
MDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVSEHSRLIAFAKENEISWAFIGPDDALAA
GIVDDFNTAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVA
ETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAFDGDKGPNTGGMGAYAPVPH
LPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIE
PYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSELLLSNGGRVYMLVTTEDSVKAGQDKIY
TQLAQQDPTGLFYRNDIGSKAIRE
>Mature_424_residues
MDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVSEHSRLIAFAKENEISWAFIGPDDALAA
GIVDDFNTAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVA
ETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAFDGDKGPNTGGMGAYAPVPH
LPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIE
PYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSELLLSNGGRVYMLVTTEDSVKAGQDKIY
TQLAQQDPTGLFYRNDIGSKAIRE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=42.8235294117647, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=42.8235294117647, Blast_Score=347, Evalue=9e-96,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=42.8235294117647, Blast_Score=347, Evalue=9e-96,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=42.8235294117647, Blast_Score=347, Evalue=9e-96,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=45.6674473067916, Blast_Score=354, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=37.793427230047, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321203, Length=448, Percent_Identity=38.8392857142857, Blast_Score=303, Evalue=5e-83,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=42.3611111111111, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=42.3611111111111, Blast_Score=303, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45847; Mature: 45847

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVSEHSRLIA
CCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCHHHHHHHHHCCCEEEE
FAKENEISWAFIGPDDALAAGIVDDFNTAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVP
EEECCCEEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCHHCCHHHHHHHHHEEEEECCC
TAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETVEQAVEAAQEMLLDNKFG
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEEHHHHHHHHHHHHHHHHCCCCC
DSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAFDGDKGPNTGGMGAYAPVPH
CCCHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCC
LPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFGDPETQII
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEEECCCCEEEEECCCCCCCCCEEE
LPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDII
EHHHHHHHHHCHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
TYYAGVKFSENSELLLSNGGRVYMLVTTEDSVKAGQDKIYTQLAQQDPTGLFYRNDIGSK
EEEECEEECCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCH
AIRE
HHCC
>Mature Secondary Structure
MDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVSEHSRLIA
CCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCHHHHHHHHHCCCEEEE
FAKENEISWAFIGPDDALAAGIVDDFNTAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVP
EEECCCEEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCHHCCHHHHHHHHHEEEEECCC
TAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETVEQAVEAAQEMLLDNKFG
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEEHHHHHHHHHHHHHHHHCCCCC
DSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAFDGDKGPNTGGMGAYAPVPH
CCCHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCC
LPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFGDPETQII
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEEECCCCEEEEECCCCCCCCCEEE
LPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDII
EHHHHHHHHHCHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
TYYAGVKFSENSELLLSNGGRVYMLVTTEDSVKAGQDKIYTQLAQQDPTGLFYRNDIGSK
EEEECEEECCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCH
AIRE
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]