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The map label for this gene is trmE [H]

Identifier: 218931079

GI number: 218931079

Start: 4622964

End: 4624328

Strand: Direct

Name: trmE [H]

Synonym: YPO4103

Alternate gene names: 218931079

Gene position: 4622964-4624328 (Clockwise)

Preceding gene: 218931078

Following gene: 218931080

Centisome position: 99.34

GC content: 51.21

Gene sequence:

>1365_bases
ATGAGCACCACTGATACTATCGTGGCCCAAGCAACGCCCCCTGGTCGTGGCGGTGTCGGCATTCTACGTGTTTCAGGTCG
TGCTGCCTCAGAGGTTGCACACGCCGTTCTGGGTAAATTACCTAAGCCACGTTATGCCGATTATCTGCCGTTTAAAGACG
TGGATGGCAGCACGCTGGATCAAGGTATCGCTCTCTATTTCCCCGGTCCAAATTCTTTCACTGGCGAAGATGTATTAGAG
CTGCAAGGCCATGGTGGGCCGGTGATACTGGATTTACTGTTAAAACGCATTCTGGCCCTGCCAGGGCTGCGTATTGCCCG
CCCAGGGGAGTTTTCCGAGCGTGCCTTCCTCAACGACAAGCTGGACTTAGCACAGGCTGAAGCCATTGCCGACCTGATCG
ACGCCAGTTCAGAGCAAGCAGCACGTTCGGCAGTAAACTCATTGCAAGGCGCATTTTCAGCGCGTATCCATCAATTGGTG
GAAGCACTTACTCACCTGAGAATCTACGTTGAAGCTGCCATTGACTTTCCAGATGAGGAAATTGACTTCCTTTCTGATGG
CAAAATCGAAGGCCAACTGAATGGTGTCATGGCCGATCTGGAACAGGTACGTACTGAGGCTAGACAAGGCAGCTTACTAC
GTGAAGGGATGAAAGTGGTGATTGCTGGCCGGCCAAACGCAGGGAAATCCAGTTTACTCAATGCGTTAGCTGGCCGTGAA
GCGGCGATTGTAACGGATATCGCGGGCACCACCCGTGATGTTTTACGGGAACACATTCATATTGATGGGATGCCTTTACA
TATCATTGATACCGCAGGGTTGCGGGAAGCCAATGACGAAGTTGAGCGCATTGGCATTGAACGCGCGTGGAACGAGATAG
AACAGGCTGATCGTGTATTGTTTATGGTGGATGGCACCACCACCGATGCCACCGAACCTGCGGCTATCTGGCCAGAGTTT
ATGGCACGCTTACCGGCAACACTGCCCATCACTGTGGTACGCAATAAAGCGGATATAACAGGTGAAACGCTGGGGCTGAC
CAAAGTGAATGGTCACTCACTTATTCGCCTGTCGGCACGCACTGGAGAAGGTATTGACCTGCTGCGTGACCATTTAAAAC
AAAGTATGGGCTTTACCAGCAACACTGAAGGCGGTTTCCTGGCTCGACGTCCCCATTTACAGGCACTGGAGACTGCCGCC
AGGCATTTGATACAAGGCCATGAGCAGTTAGTGAGCGCTTACGCTGGTGAATTACTGGCAGAAGAACTGCGTTTAGCACA
ACAATCATTGAGTGAAATTACCGGTGAATTTAGTTCTGATGACTTACTGGGGCGGATTTTTTCCAGTTTCTGTATCGGAA
AATGA

Upstream 100 bases:

>100_bases
CGCCTTATTCTTTTTACAGAAGCCTGATTTTTCACCTTCAGATTTGTGTAAAAATAATACAGACTTGTTACAAAAATAAT
CGATAAAGAGAGAAATCACC

Downstream 100 bases:

>100_bases
GTGTAATACCATAAAGCTAGCACCAGTTATCGCTAATTTTGATAACCTTACTCTATGGGTTTGGCAGTGGCTCGAACACT
TAACGTTGTATACCCTAAAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSTTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLDQGIALYFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVLFMVDGTTTDATEPAAIWPEF
MARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNTEGGFLARRPHLQALETAA
RHLIQGHEQLVSAYAGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSTTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLDQGIALYFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVLFMVDGTTTDATEPAAIWPEF
MARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNTEGGFLARRPHLQALETAA
RHLIQGHEQLVSAYAGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
>Mature_453_residues
STTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLDQGIALYFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVLFMVDGTTTDATEPAAIWPEFM
ARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNTEGGFLARRPHLQALETAAR
HLIQGHEQLVSAYAGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=35.5603448275862, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=34.20523138833, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=34.4226579520697, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=88.7665198237885, Blast_Score=814, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=39.5604395604396, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=28.7581699346405, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=31.2244897959184, Blast_Score=216, Evalue=7e-57,
Organism=Drosophila melanogaster, GI45550813, Length=490, Percent_Identity=32.0408163265306, Blast_Score=205, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48978; Mature: 48847

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLD
CCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QGIALYFPGPNSFTGEDVLELQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDATEPAAIWPEFMARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSAR
EEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCEEEEEECC
TGEGIDLLRDHLKQSMGFTSNTEGGFLARRPHLQALETAARHLIQGHEQLVSAYAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLD
CCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QGIALYFPGPNSFTGEDVLELQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDATEPAAIWPEFMARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSAR
EEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCEEEEEECC
TGEGIDLLRDHLKQSMGFTSNTEGGFLARRPHLQALETAARHLIQGHEQLVSAYAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA