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The map label for this gene is intS [H]

Identifier: 218931021

GI number: 218931021

Start: 4546613

End: 4547806

Strand: Reverse

Name: intS [H]

Synonym: YPO4033

Alternate gene names: 218931021

Gene position: 4547806-4546613 (Counterclockwise)

Preceding gene: 218931022

Following gene: 218931020

Centisome position: 97.72

GC content: 45.98

Gene sequence:

>1194_bases
ATGCCACTATCAGATAGACAGATTCGCCGTGCTAAACCACAAGAAAAAGCCTATACCCTGAGTGACGGGCAAGGGCTATC
ACTCTTAATTGAACCTAACGGCAGTAAAGGGTGGCGCTTTCGCTATCGTTTTGCCGGTAAAGCCCGATTAATGTCATTAG
GGACTTATGATCTTGTTTCTCTTGCTGAAGCTCGCTCTAAGCGAGATGTGGCACGTAAACAGGTTGCAGATGGAACAGAT
CCCGCAGAGGTGAAAAAGGCTGAGAAGTTGGCGCAGCGGCTTTCATCGGAAAACTCTTTTGAGGCCATTAGCCGTGAGTG
GCATAAAGCCAAAGCCGATCGCTGGTCATTGGGCTATAGGGAAGAAATCATGAGTACCTTTGAGGCGGATATATTCCCGT
ATATTGGTAAACGGCCAATTGCAGAAATCACTCCGCTGGAGTTACTTGATGTGCTTCAACGTATCGAAAAGCGTGGAGCG
TTAGAAAAAACGCGCAAAGTACGCCAGCGCTGTGGTGAAGTATTTCGTTATGCCATTATTACCGGCCGTGCTGAATATAA
TCCCGCCCCTGACCTTGCCAGCGCATTAAGTACACCAAAGAAACAGCACTACCCGTTCCTGTCTGCCGAAGAGATGCCAT
ATTTTATTCGTGATCTAGAGGGTTACACCGGCAGCATCATAACCAAGAACGCGGCAAAGATACTTATGCTAACGGGTGTG
CGAACTAAGGAAATGCGTTTTGCTACTTGGCAGGAAATCGATCTTGAGGGCGGCTTGTGGGAGATTCCAGCAGAACGAAT
GAAGATGCGCCGCCCCCATATCGTGCCGCTGTCTACACAAGTTATAGCGCTATTTAAACAGCTCTTACCTATCACCGGGC
ATTACCCTTATATTTTCATTGGACTGAATGACCGTAAAAAGCCCATAAGTAAAGAAACAGTCAATCAGGTCATTGAATTA
CTGGGTTATAAAGGCCGTGCGACGGGTCACGGATTTAGACATACCATGTCAACGATATTGCATGAGCAGGGCTATGATAG
TGCATGGATTGAGCTACAATTGGCTCACGTTGATAAGAACAGTATTCGTGGTACTTACAATCATGCGCAGTATCTTGAAA
AGCGAAGAGAGATGTTGCAGTGGTATGCTGATCTCATTTTTAATTTTTTCAGGAAAAATTATGAGCGCATCTAA

Upstream 100 bases:

>100_bases
CACTTACTGTCTGTGGTGTTCCGAGTTGATCTCCTCAAATCTGCAACAAATGTGTATAGCAATGTGTATAGGATTTTATG
GTGATTTTTGAGCTATACAC

Downstream 100 bases:

>100_bases
GCAAGATGTACAAGATTTTGAGTTTATAATTTCAGAGGCTATCAGAATTAAGGATAGTTTGAGAAATGAAGACTTTAAAT
CTTTTATGGCGCAAGAATAT

Product: putative phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MPLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVSLAEARSKRDVARKQVADGTD
PAEVKKAEKLAQRLSSENSFEAISREWHKAKADRWSLGYREEIMSTFEADIFPYIGKRPIAEITPLELLDVLQRIEKRGA
LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLASALSTPKKQHYPFLSAEEMPYFIRDLEGYTGSIITKNAAKILMLTGV
RTKEMRFATWQEIDLEGGLWEIPAERMKMRRPHIVPLSTQVIALFKQLLPITGHYPYIFIGLNDRKKPISKETVNQVIEL
LGYKGRATGHGFRHTMSTILHEQGYDSAWIELQLAHVDKNSIRGTYNHAQYLEKRREMLQWYADLIFNFFRKNYERI

Sequences:

>Translated_397_residues
MPLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVSLAEARSKRDVARKQVADGTD
PAEVKKAEKLAQRLSSENSFEAISREWHKAKADRWSLGYREEIMSTFEADIFPYIGKRPIAEITPLELLDVLQRIEKRGA
LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLASALSTPKKQHYPFLSAEEMPYFIRDLEGYTGSIITKNAAKILMLTGV
RTKEMRFATWQEIDLEGGLWEIPAERMKMRRPHIVPLSTQVIALFKQLLPITGHYPYIFIGLNDRKKPISKETVNQVIEL
LGYKGRATGHGFRHTMSTILHEQGYDSAWIELQLAHVDKNSIRGTYNHAQYLEKRREMLQWYADLIFNFFRKNYERI
>Mature_396_residues
PLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVSLAEARSKRDVARKQVADGTDP
AEVKKAEKLAQRLSSENSFEAISREWHKAKADRWSLGYREEIMSTFEADIFPYIGKRPIAEITPLELLDVLQRIEKRGAL
EKTRKVRQRCGEVFRYAIITGRAEYNPAPDLASALSTPKKQHYPFLSAEEMPYFIRDLEGYTGSIITKNAAKILMLTGVR
TKEMRFATWQEIDLEGGLWEIPAERMKMRRPHIVPLSTQVIALFKQLLPITGHYPYIFIGLNDRKKPISKETVNQVIELL
GYKGRATGHGFRHTMSTILHEQGYDSAWIELQLAHVDKNSIRGTYNHAQYLEKRREMLQWYADLIFNFFRKNYERI

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=385, Percent_Identity=47.5324675324675, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI145693166, Length=395, Percent_Identity=41.0126582278481, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1788974, Length=387, Percent_Identity=37.4677002583979, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45949; Mature: 45817

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVS
CCCCCCHHHHCCCHHHCEEECCCCCCEEEEECCCCCCCEEEEEECCCHHEEECCCHHHHH
LAEARSKRDVARKQVADGTDPAEVKKAEKLAQRLSSENSFEAISREWHKAKADRWSLGYR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHH
EEIMSTFEADIFPYIGKRPIAEITPLELLDVLQRIEKRGALEKTRKVRQRCGEVFRYAII
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRAEYNPAPDLASALSTPKKQHYPFLSAEEMPYFIRDLEGYTGSIITKNAAKILMLTGV
CCCCCCCCCCHHHHHHCCCHHHCCCCCCCCCCCHHHHHHCCCCCCEEECCCCEEEEEECC
RTKEMRFATWQEIDLEGGLWEIPAERMKMRRPHIVPLSTQVIALFKQLLPITGHYPYIFI
CHHHHHHHHHHEEECCCCCEECCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
GLNDRKKPISKETVNQVIELLGYKGRATGHGFRHTMSTILHEQGYDSAWIELQLAHVDKN
EECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SIRGTYNHAQYLEKRREMLQWYADLIFNFFRKNYERI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVS
CCCCCHHHHCCCHHHCEEECCCCCCEEEEECCCCCCCEEEEEECCCHHEEECCCHHHHH
LAEARSKRDVARKQVADGTDPAEVKKAEKLAQRLSSENSFEAISREWHKAKADRWSLGYR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHH
EEIMSTFEADIFPYIGKRPIAEITPLELLDVLQRIEKRGALEKTRKVRQRCGEVFRYAII
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRAEYNPAPDLASALSTPKKQHYPFLSAEEMPYFIRDLEGYTGSIITKNAAKILMLTGV
CCCCCCCCCCHHHHHHCCCHHHCCCCCCCCCCCHHHHHHCCCCCCEEECCCCEEEEEECC
RTKEMRFATWQEIDLEGGLWEIPAERMKMRRPHIVPLSTQVIALFKQLLPITGHYPYIFI
CHHHHHHHHHHEEECCCCCEECCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
GLNDRKKPISKETVNQVIELLGYKGRATGHGFRHTMSTILHEQGYDSAWIELQLAHVDKN
EECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SIRGTYNHAQYLEKRREMLQWYADLIFNFFRKNYERI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]