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The map label for this gene is aceF [H]

Identifier: 218930435

GI number: 218930435

Start: 3818758

End: 3820287

Strand: Reverse

Name: aceF [H]

Synonym: YPO3418

Alternate gene names: 218930435

Gene position: 3820287-3818758 (Counterclockwise)

Preceding gene: 218930436

Following gene: 218930434

Centisome position: 82.09

GC content: 51.63

Gene sequence:

>1530_bases
ATGTCTATAGAAATTAATGTACCAGACATCGGTGCAGATGAAGTGGAAGTCACCGAAATTATGGTGAAAGTGGGCGATAC
CGTTGAAGCGGAACAGTCGCTAATCACCGTTGAAGGCGATAAAGCTTCCATGGAAGTTCCTTCACCTCAGGCGGGCGTGG
TTAAAGAGATCAAAATTGCGGTTGGCGATAAAGTGGCTACCGGTTCTCTGATCATGGTCTTCGACGCTACGGGTGCCGCT
GCGGCACCGGTTAAAGCAGAAGAAAAACCGGCGGCGCCTGCTCAGGTAGCGGCTCCGGCAGCCTCTGCGGCGAAAAATGT
TGAAGTGCCAGATATCGGTGATGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGATAAAGTTGACGCCGAAC
AATCACTGATTACGGTTGAAGGCGACAAAGCGTCGATGGAAGTGCCCGCACCGTTTGCTGGTATCGTGAAAGAAATCAAA
ATCAGTACCGGCGACAAAGTGAAAACCGGCTCTCTGATTATGGTCTTCGAAGTTGAAGGTGCAGCGCCAGCGCCAGCCGC
CGCACCTGCCGCCAAAGCAGAAAGCAAAGGCGAGTTTGCCGAGAATGACGCTTACGTGCATGCCACGCCGGTTATCCGTC
GTCTGGCGCGTGAGTTCGGTGTGAACCTGGCGAAGGTGAAAGGGACAGGCCGTAAGGGCCGTATCCTGCGCGAAGACATT
CAAGCTTACGTGAAAGATGCCGTGAAACGTGCCGAAGCTGCACCAGCAGCGGCTGGCGGCGGCCTGCCGGGCATATTGCC
TTGGCCAAAAGTTGATTTCAGTAAATTTGGTGAAATCGAAGAAGTCGAATTGGGCCGTATCCAGAAAATTTCTGGTGCGA
ACCTGAGCCGTAACTGGGTCATGATCCCACATGTGACGCAATTCGATGAAGCGGATATCACTGAAGTTGAAGCCTTCCGT
AAGCAACAGAACATCGAAGCTGAGAAGAAAAAACAAGACCTGAAAATCACCCCGCTGGTGTTCCTGATGAAGGCCGCCGC
TAAAGCACTGGAAGAATTCCCACGCTTTAACAGCTCCATTTCCGAAGATGGTCAGAAACTGACGCTGAAGAAATACATCA
ATATCGGTGTGGCGGTTGATACGCCTAACGGCTTGGTAGTTCCAGTATTCCGTGACGTCAACAAAAAGGGTATTGTCGAG
TTGTCTCGTGAGCTATCTGTCATCTCCAAGAAAGCACGTGATGGCAAGCTGACAGCATCTGACATGCAAGGCGGCTGTTT
CACTATCTCCAGTCTGGGCGGTATCGGCGGTACGGCATTTACGCCAATCGTCAATGCGCCAGAAGTGGCTATCTTGGGTG
TATCAAAATCATCCATGAAACCTGTCTGGAATGGTAAAGAGTTTGCTCCACGCCTGATGTTACCGCTGTCTCTGTCCTTC
GATCACCGTGTGATTGATGGTGCCGCGGGTGCACGCTTCGCCGCGTATATCGCTACCATTATGGCGGATATTCGCCGTCT
GGTGATGTAA

Upstream 100 bases:

>100_bases
CGCGGTGACATCGACACCAGTGTAGTTGCTGAAGCAATTACTAAGTTTGGTATCGACGCTGATAAAGTTAACCCGCGTCT
GGCATAAGAGGTAGAGAATA

Downstream 100 bases:

>100_bases
TCGCCAAGGCCGGCTTCGTGCCGGCCTTGTTGTGGTTACTGCTCTTGTTATTGGTGATCTTGTTATTACTGATCACCAAT
AGAGAAAAGACACTTATAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAA
AAPVKAEEKPAAPAQVAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIK
ISTGDKVKTGSLIMVFEVEGAAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDI
QAYVKDAVKRAEAAPAAAGGGLPGILPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTQFDEADITEVEAFR
KQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVE
LSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVWNGKEFAPRLMLPLSLSF
DHRVIDGAAGARFAAYIATIMADIRRLVM

Sequences:

>Translated_509_residues
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAA
AAPVKAEEKPAAPAQVAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIK
ISTGDKVKTGSLIMVFEVEGAAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDI
QAYVKDAVKRAEAAPAAAGGGLPGILPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTQFDEADITEVEAFR
KQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVE
LSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVWNGKEFAPRLMLPLSLSF
DHRVIDGAAGARFAAYIATIMADIRRLVM
>Mature_508_residues
SIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAAA
APVKAEEKPAAPAQVAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKI
STGDKVKTGSLIMVFEVEGAAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDIQ
AYVKDAVKRAEAAPAAAGGGLPGILPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTQFDEADITEVEAFRK
QQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVEL
SRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVWNGKEFAPRLMLPLSLSFD
HRVIDGAAGARFAAYIATIMADIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=418, Percent_Identity=31.5789473684211, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI31711992, Length=413, Percent_Identity=30.7506053268765, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI19923748, Length=206, Percent_Identity=38.3495145631068, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI203098816, Length=442, Percent_Identity=27.1493212669683, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI203098753, Length=443, Percent_Identity=26.86230248307, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI260898739, Length=142, Percent_Identity=37.3239436619718, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786305, Length=525, Percent_Identity=76.7619047619048, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=395, Percent_Identity=32.1518987341772, Blast_Score=179, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=406, Percent_Identity=29.8029556650246, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=210, Percent_Identity=38.0952380952381, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=27.5058275058275, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=27.5641025641026, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=414, Percent_Identity=29.2270531400966, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=338, Percent_Identity=27.5147928994083, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=30.9352517985612, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=36.4485981308411, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 53950; Mature: 53819

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIA
CEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMVFDATGAAAAPVKAEEKPAAPAQVAAPAASAAKNVEVPDIGDDEVEVT
ECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCHHHHH
EVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKISTGDKVKTGSLIMVFEVEG
HHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHCCCCCCCEECCCEEEEEEECC
AAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDI
CCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
QAYVKDAVKRAEAAPAAAGGGLPGILPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCEE
MIPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSI
EECCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCH
SEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVELSRELSVISKKARDGKLTAS
HCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECC
DMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVWNGKEFAPRLMLPLSLSF
CCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEEEECCC
DHRVIDGAAGARFAAYIATIMADIRRLVM
CCEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIA
EEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMVFDATGAAAAPVKAEEKPAAPAQVAAPAASAAKNVEVPDIGDDEVEVT
ECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCHHHHH
EVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKISTGDKVKTGSLIMVFEVEG
HHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHCCCCCCCEECCCEEEEEEECC
AAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDI
CCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
QAYVKDAVKRAEAAPAAAGGGLPGILPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCEE
MIPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSI
EECCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCH
SEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVELSRELSVISKKARDGKLTAS
HCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECC
DMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVWNGKEFAPRLMLPLSLSF
CCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEEEECCC
DHRVIDGAAGARFAAYIATIMADIRRLVM
CCEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]