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The map label for this gene is speE [H]

Identifier: 218930428

GI number: 218930428

Start: 3809283

End: 3810173

Strand: Direct

Name: speE [H]

Synonym: YPO3411

Alternate gene names: 218930428

Gene position: 3809283-3810173 (Clockwise)

Preceding gene: 218930427

Following gene: 218930429

Centisome position: 81.85

GC content: 48.48

Gene sequence:

>891_bases
ATGTCACAGAAAGAACTATGGTATGAAACATTACATGCCAACTTCGGCCAGTACTTTTCTGTTGAGAATGTATTGTTCCG
CGAAAAAACCGAGCATCAGGACCTGGTGATTTTTGAAAACCCAGAGTTGGGCCGCGTAATGGCATTAGATGGTGTGGTGC
AAACGACAGAGCGTGATGAGTTTATCTACCACGAAATGATGACACACGTTCCTTTGCTGGCCCACGGGCAAGCAAAAAAA
GTGCTGATCATCGGCGGTGGCGATGGCGCAATGCTGCGAGAAGTCAGCCGCCACAAGAATATTGAGCAGATTACCATGGT
GGAAATTGACGCCGGGGTCGTAGAATTTTGCCGCCAGTATTTGCCTAATCATAGCGCGGGGGCGTATGACGATCCTCGTT
TCAAGCTAGTCATCGACGATGGTGTGAATTTCGTTAATCAGACCACTGAAAAATTCGATGTCATCATTTCTGACTGTACC
GATCCTATCGGTCCTGGCGAAAGCCTGTTTACCTCTGTATTTTATGAGGGATGCGCCCGTAGCCTGAATGAGGGGGGGAT
CTTCGTTGCACAAAACGGCGTCTGTTTCCTACAACAGGATGAAGCCGTCAATAGCCATAACAAACTTAGCCACTATTTCA
GTGACGTTAGTTTTTATCAGGCCGCAATTCCAACGTATTACGGCGGTATTATGACCTTTGCCTGGGCGACACAGAACCCG
GCTCTGCGCCAGTTGGATCTCGCCACCTTGCAAAACCGCTTTGCCCAAGCTGGCTTGGCCTGCCGCTACTATAACCCAGC
GATTCATGTCGGCAGCTTTGCTTTACCACAGTATTTGCTAGATGCCTTAACGACGATACCCAAAGTCATTGGCGTCGATT
CAAGTGAATAA

Upstream 100 bases:

>100_bases
GCCTTTTGCCTGAAATTTGACCGTGCAACCTCAGGCAGAGTTGGCTATGATATTGCCCCCCATTTTTCATGGCTGTTACT
GCGAAGGAGAAGCCCCGAAC

Downstream 100 bases:

>100_bases
GAGCCGCTAGCAACGCAGTAGTGCCGAGTAAAAAAGGGATAAATGTTGAGTATTCATTTGTAAGGAGGTGAACTAAATTG
TCCAAGCTTAAACTGCACGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSQKELWYETLHANFGQYFSVENVLFREKTEHQDLVIFENPELGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKK
VLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQYLPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCT
DPIGPGESLFTSVFYEGCARSLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE

Sequences:

>Translated_296_residues
MSQKELWYETLHANFGQYFSVENVLFREKTEHQDLVIFENPELGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKK
VLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQYLPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCT
DPIGPGESLFTSVFYEGCARSLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE
>Mature_295_residues
SQKELWYETLHANFGQYFSVENVLFREKTEHQDLVIFENPELGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKKV
LIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQYLPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCTD
PIGPGESLFTSVFYEGCARSLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNPA
LRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=288, Percent_Identity=37.1527777777778, Blast_Score=197, Evalue=9e-51,
Organism=Escherichia coli, GI1786312, Length=288, Percent_Identity=80.2083333333333, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25154106, Length=270, Percent_Identity=39.6296296296296, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI212645616, Length=226, Percent_Identity=41.1504424778761, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=38.1118881118881, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6325326, Length=275, Percent_Identity=35.2727272727273, Blast_Score=179, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24645443, Length=278, Percent_Identity=38.1294964028777, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=36.2790697674419, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 33167; Mature: 33036

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKELWYETLHANFGQYFSVENVLFREKTEHQDLVIFENPELGRVMALDGVVQTTERDE
CCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHCCCCHH
FIYHEMMTHVPLLAHGQAKKVLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQY
HHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCTDPIGPGESLFTSVFYEGCAR
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
HCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE
CHHHHHHHHHHHHHHHHCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQKELWYETLHANFGQYFSVENVLFREKTEHQDLVIFENPELGRVMALDGVVQTTERDE
CCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHCCCCHH
FIYHEMMTHVPLLAHGQAKKVLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQY
HHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCTDPIGPGESLFTSVFYEGCAR
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
HCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE
CHHHHHHHHHHHHHHHHCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA