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The map label for this gene is yacK

Identifier: 218930426

GI number: 218930426

Start: 3806901

End: 3808502

Strand: Reverse

Name: yacK

Synonym: YPO3409

Alternate gene names: 218930426

Gene position: 3808502-3806901 (Counterclockwise)

Preceding gene: 218930431

Following gene: 218930425

Centisome position: 81.84

GC content: 53.25

Gene sequence:

>1602_bases
ATGCATCGCCGTGATTTTCTTAAGTTAACGGCCGCTCTTGGAGCTGCCACATCACTGCCTTTATGGAGCCGAGCGGCATT
GGCCGCAGATTTTTCCCCGTTACCCATTCCCCCTCTGCTCCAACCGGATGCCAACGGTAAAATTAATCTGAATATTCAGA
CGGGGAGTGTGGTCTGGTTACCTTCCACTGCGACGCAAACCTGGGGCTATAACGGTAATTTATTGGGTCCAGCGATTCGT
TTGCAGCGGGGTAAAGCGGTAACCATTGATATCACTAATGCTTTACCGGAAGCGACCACAGTACATTGGCATGGTTTGGA
GATCCCCGGCGAGGTTGATGGTGGCCCACAGGCGTTGATTCAGCCAGGGGCAAAGCGTCAGGTTACCTTCGCGGTGGAGC
AACCCGCCGCAACGTGTTGGTTCCATCCGCACACTCACAGTAAAACGGGTCACCAAGTGGCGATGGGGTTAGGCGGGTTA
GTCCTGATTGATGACAGCGACAGTGAGACGCTGCCGTTGCCAAAACAGTGGGGCGTGGACGATATTCCCGTAATTTTGCA
GGATAAGTTACTCGATAAACATGGGCAGGTTGACTATCAGCTTGATGTGATGACCGCCGCAGTCGGCTGGTTTGGTGACC
GGATGCTGACTAACGGCGTTCCTTATCCGCAACAAATTACGCCACGTGGCTGGGTGCGATTACGGCTACTTAATGGCTGT
AATGCCCGTTCGCTGAATCTGGCGCTTAGCGATGGCCGGCCAATGTATGTGATTGCCAGCGACGGCGGATTATTAGCCGA
ACCCGTTGTGGTGCGTGAGTTACCGATATTGATGGGCGAACGTTTCGAAGTGCTGGTGGATACCCGCGATGGTCAGTCTC
TCGATTTGGTCACCCTGCCCGTTACGCAGATGGGCATGACCTTGGCCCCGTTTGATCAGCCGCTGCCCGTGCTACGGATC
CAACCCTCACTGGCGATCGGCAGTCAGGTTTTGCCCGAGTCTCTCGTGGTGATCCCGGAATTAGCCGATGTCACTGGTGT
GCAGGAGCGCTGGTTCCAACTGATGATGGATCCAAAGCTCGATATGCTGGGGATGCAGGCCTTAGTGGCGCGTTATGGCA
TGAAAGCCATGGCCGGTATGAATATGAATCATGGTGACATGGGGGCGATGGACCATGGCAATAGACCAGATATGAGCCAG
GGCAAAATGAAAGGCATGGATCATGGCACAATGAACGGTGCGCCAGCCTTTAATTTCAGTCACGCGAATAGGATTAACGG
TAAAGCTTTCTCGATGACCGAACCCGCGTTTGACGCGAAGCAGGGCAAATATGAGAAATGGACCATTTCAGGTGAAGGCG
ACATGATGCTACATCCATTCCATGTTCACGGCACACAGTTCCGTATTTTAACGGAGAACGGCAAACCGCCAGCAGAGCAT
CGCCGGGGATGGAAAGACATAGTACGTGTTGAAGGCGCACGCAGTGAAATATTGGTGCGCTTTAATTATCTCGCCCCTGC
GAGTACGCCTTATATGGCTCACTGCCACTTATTGGAACATGAAGATACTGGCATGATGCTGGGCTTTACCGTCAGCGCCT
GA

Upstream 100 bases:

>100_bases
GGTTTTTATATCCATATATCCATGTGCTATGCAGGTATTTACCTACGGTCAGCATTTACCCATGATCAGCATTTACTTAT
CATCAAGAAGGGAGACGACC

Downstream 100 bases:

>100_bases
GCCACACCCCGGTCGGTTGACCGGGGTGTTTTTTATAAAACCGCCATCAATGCATCCCACTGCGCATTTTTGTTATACTT
TGTCTCCCCTAAGCTGGCAG

Product: multicopper oxidase

Products: NA

Alternate protein names: Copper efflux oxidase

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWLPSTATQTWGYNGNLLGPAIR
LQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALIQPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGL
VLIDDSDSETLPLPKQWGVDDIPVILQDKLLDKHGQVDYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLPVTQMGMTLAPFDQPLPVLRI
QPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDPKLDMLGMQALVARYGMKAMAGMNMNHGDMGAMDHGNRPDMSQ
GKMKGMDHGTMNGAPAFNFSHANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
RRGWKDIVRVEGARSEILVRFNYLAPASTPYMAHCHLLEHEDTGMMLGFTVSA

Sequences:

>Translated_533_residues
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWLPSTATQTWGYNGNLLGPAIR
LQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALIQPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGL
VLIDDSDSETLPLPKQWGVDDIPVILQDKLLDKHGQVDYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLPVTQMGMTLAPFDQPLPVLRI
QPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDPKLDMLGMQALVARYGMKAMAGMNMNHGDMGAMDHGNRPDMSQ
GKMKGMDHGTMNGAPAFNFSHANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
RRGWKDIVRVEGARSEILVRFNYLAPASTPYMAHCHLLEHEDTGMMLGFTVSA
>Mature_533_residues
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWLPSTATQTWGYNGNLLGPAIR
LQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALIQPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGL
VLIDDSDSETLPLPKQWGVDDIPVILQDKLLDKHGQVDYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLPVTQMGMTLAPFDQPLPVLRI
QPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDPKLDMLGMQALVARYGMKAMAGMNMNHGDMGAMDHGNRPDMSQ
GKMKGMDHGTMNGAPAFNFSHANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
RRGWKDIVRVEGARSEILVRFNYLAPASTPYMAHCHLLEHEDTGMMLGFTVSA

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains

Homologues:

Organism=Escherichia coli, GI1786314, Length=531, Percent_Identity=64.9717514124294, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI1789394, Length=526, Percent_Identity=32.6996197718631, Blast_Score=249, Evalue=4e-67,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): CUEO_YERPE (Q8ZBK0)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AC0414
- RefSeq:   YP_002348301.1
- ProteinModelPortal:   Q8ZBK0
- SMR:   Q8ZBK0
- IntAct:   Q8ZBK0
- GeneID:   1176226
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3409
- KEGG:   ypk:y0777
- KEGG:   ypm:YP_0276
- HOGENOM:   HBG655385
- OMA:   LLKFRIH
- ProtClustDB:   PRK10965
- BioCyc:   YPES187410:Y0777-MONOMER
- BioCyc:   YPES214092:YPO3409-MONOMER
- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.60.40.420
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3; SSF49503 Cupredoxin

EC number: NA

Molecular weight: Translated: 58329; Mature: 58329

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00080 MULTICOPPER_OXIDASE2; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWL
CCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEE
PSTATQTWGYNGNLLGPAIRLQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALI
CCCCCCCCCCCCCEECCEEEEECCCEEEEEECCCCCCCEEEEEECEECCCCCCCCCHHHC
QPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGLVLIDDSDSETLPLPKQWGVD
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHCCCC
DIPVILQDKLLDKHGQVDYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
CCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLP
CCEEEEEEECCCCEEEEEECCCCCEECCCHHHHCCHHHCCCEEEEEECCCCCEEEEEEEC
VTQMGMTLAPFDQPLPVLRIQPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDPKL
HHHCCCEECCCCCCCCEEEECCCHHCCCHHCCCCEEEEECHHHHCCHHHHHHHHHCCCCH
DMLGMQALVARYGMKAMAGMNMNHGDMGAMDHGNRPDMSQGKMKGMDHGTMNGAPAFNFS
HHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
CCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEEECCEEEEEEECCCCCCHHH
RRGWKDIVRVEGARSEILVRFNYLAPASTPYMAHCHLLEHEDTGMMLGFTVSA
HCCHHHHHEECCCCCEEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWL
CCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEE
PSTATQTWGYNGNLLGPAIRLQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALI
CCCCCCCCCCCCCEECCEEEEECCCEEEEEECCCCCCCEEEEEECEECCCCCCCCCHHHC
QPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGLVLIDDSDSETLPLPKQWGVD
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHCCCC
DIPVILQDKLLDKHGQVDYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
CCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLP
CCEEEEEEECCCCEEEEEECCCCCEECCCHHHHCCHHHCCCEEEEEECCCCCEEEEEEEC
VTQMGMTLAPFDQPLPVLRIQPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDPKL
HHHCCCEECCCCCCCCEEEECCCHHCCCHHCCCCEEEEECHHHHCCHHHHHHHHHCCCCH
DMLGMQALVARYGMKAMAGMNMNHGDMGAMDHGNRPDMSQGKMKGMDHGTMNGAPAFNFS
HHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
CCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEEECCEEEEEEECCCCCCHHH
RRGWKDIVRVEGARSEILVRFNYLAPASTPYMAHCHLLEHEDTGMMLGFTVSA
HCCHHHHHEECCCCCEEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430