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The map label for this gene is mrcB [H]

Identifier: 218930410

GI number: 218930410

Start: 3788401

End: 3790875

Strand: Reverse

Name: mrcB [H]

Synonym: YPO3393

Alternate gene names: 218930410

Gene position: 3790875-3788401 (Counterclockwise)

Preceding gene: 218930411

Following gene: 218930409

Centisome position: 81.46

GC content: 53.37

Gene sequence:

>2475_bases
ATGTCTGGGGACGATCGTGAGCCGATAGGGCGCAAGGGCAAGAAAGCCCAACCCAAAGCAGCACCAAAACGTCCACTGCG
CCGTCGGCGTGATGAAGATGAATACGAAGAAGATTATGACGAAGAACAAAATGATTATGATGACGAGGAGGAACCGATGC
CAAGAAAAGTAGCATCACGCCCACCTCGCAAGAAACGCCGTTGGCTCGGGTTGTTGATCAAACTGTTCCTGATTGGGGCC
GTGGCTTTGGCGATCTATGGGGTTTACCTCGATTCGCAAATCCGCAGCCGTATTGATGGCAAAGTCTGGCAATTACCGGC
TGCCGTGTATGGCCGCATGGTCAATCTTGAGCCGGGTATGTCCTACAGCAAAAAAGAGATGATCGCCTTGCTGGAAGGGA
TGCAATACCGTCAGGTCAGTCGCATGACCCGCCCTGGGGAGTTCACGGTTCAGAACGACAGTATCGATATCTTGCGCCGG
CCGTTCGATTTCCCTGATGGTAAAGAAGGCCAAATTCGCGCCCGTTTACTGTTCAAAAATGATCGTCTGGCCCAGATTCA
AAACCTGGATAATCAGCGTGATTTCGGGCTATTCCGCCTCGATCCCAAGTTGATTACCATGCTGCAATCACCTAACGGTG
AACAGCGTCTGTTTGTTCCGCGCGCCGGGTTCCCTGATTTGCTGGTGGACACCTTGCTGGCGACTGAAGACCGCCATTTC
TATGAGCATGATGGCGTCAGCCCGTATTCCATTGCTCGAGCCGTGGTCGCTAACCTGACAGCCGGTAAAGCGGTGCAGGG
GGGCAGTACGCTGACGCAACAGTTAGTGAAAAACCTGTTCCTGACCAATGAACGTTCGCTGGTACGTAAACTTAACGAGG
CCTATATGGCCCTGTTGATGGATTATCGCTACAGCAAAGATCGGATCTTGGAACTCTATCTGAACGAAGTCTATCTTGGT
CAGAGTGGTGGTGACCAAATCCGTGGCTTCCCGCTGGGTAGCTTGTATTACTTTGGCCGCCCGGTTGATGAATTGAGTCT
CGATCAGCAAGCCATGTTGGTTGGCATGGTGAAAGGGGCCTCACTGTATAACCCGTGGCGTAATCCTAAGCTGGCACTTG
AGCGGCGTAATTTGGTCCTGCGCTTGTTGCAGAATCAAGGGATTATCGATGCTGAGCTTTACACCATGCTCAGTGCCCGT
CCGCTGGGGGTGCAGCCGAAAGGCGGAGTCATCACACCTCAACCTGCTTTTATGCAGATGGTGCGCCAGGAGCTGCAACA
GAAGTTGGGTAACAAGGTCAATGATCTGTCTGGCGTGAAGATCTTCACCACCTTGGATCCGGTTTCACAAGATGCCGCAG
AAAAAGCCATCGAAGACGGTGTTCCGGCGCTGAGAGCCGCCCGTAATATGAATGACCTGGAAGCGGCGATGGTGGTGGTT
GACCGTTTCAGTGGCGAAGTGCGTGCCATGGTGGGCGGTTCACAGCCACAATTTGCCGGTTTTAACCGTGCGATGCAGGC
CCGCCGTTTGGTGGGGTCACTTGCCAAACCACCAACCTATCTGGCCGCGTTGAGTGAGCCGGATAAGTACCGCCTCAATA
CCTGGCTTTCGGACCAACCGCTGTCGCTTAAATTGTCGAACGGTTCATTGTGGCAGCCGAAAAACTATGATCGCCAGTTC
CGTGGTCAGGTCATGTTGATGGATGCGTTGGTGAACTCACTGAACATCCCAACAGTCAATTTGGGGATGTCAGTGGGCTT
AGATCAGATCAGCGCCACACTGCAACGCCTTGGGATCCCGAAATCAGTCATTAATCCGGTCCCAGCCATGTTATTGGGGG
CGATTGACCTGACTCCGGTTGAAGTTGCGCAGGAGTATCAGACAATCGCCAGTGGTGGCAACCGTGCACCGTTGTCGGCT
GTGCGTTCGGTTATTGCTGAGGATGGGACCGTGCTGTATCAGAGCTTCCCACAGGCAGAACGTATGGTGCCTGCACAAGC
TTCTTACCTGACGCTGTATGCGATGCAGCAAGGGGTTGTCCGTGGTACATCACGCTCTCTCTCGGCGAAGTTCGGCAAAT
ATAATCTGGCGGCCAAAACCGGGACAACCAACGATCTGCGTGACAGTTGGTTTGCCGGTATTGATGGCAAAGAAGTGACC
ATTGCCTGGATTGGGCGCGACAATAACGGCCCGACTAAGCTGACCGGGGCCAGCGGCGCGCTAACCTTGTATCGCCGCTA
TCTGGAAAACCAAACGCCGTTGCCGCTGATTCTGCAACCGCCAGAAGGCATTAGCCAAATGAACATTGATTCGGCGGGGA
ACTTCGTCTGTGGTGAAGGGAGCGGGATGCGCGTCATTCCTGTTTGGACCGAAAATCCGCAAGCTTTGTGTCAGGGATCA
ACACCCACTCAAGACCCCACAAAACCTAACGATGATGGTGTTGCCGATTGGATCAAAGAGATGTTTGGCCAATAA

Upstream 100 bases:

>100_bases
GGCTGATAGCCAATAAATTCAGATGTTTCTCGTTTCTATCCGGTGAATTGGGGAACACATTAAGCCGCAATAATCACCAA
TGCGCGTGGAGATAGGAAAC

Downstream 100 bases:

>100_bases
AATGTGTGGTCAATAAAATGTGACGGCGGGCGTGGTTTACAGACTGCGCCCGCCAATTTTTTTCTTTGCGCTTCCCTTCT
TCGTTTCCCCCAACAAATCA

Product: penicillin-binding protein 1b

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 824; Mature: 823

Protein sequence:

>824_residues
MSGDDREPIGRKGKKAQPKAAPKRPLRRRRDEDEYEEDYDEEQNDYDDEEEPMPRKVASRPPRKKRRWLGLLIKLFLIGA
VALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMSYSKKEMIALLEGMQYRQVSRMTRPGEFTVQNDSIDILRR
PFDFPDGKEGQIRARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNGEQRLFVPRAGFPDLLVDTLLATEDRHF
YEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLNEAYMALLMDYRYSKDRILELYLNEVYLG
QSGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGASLYNPWRNPKLALERRNLVLRLLQNQGIIDAELYTMLSAR
PLGVQPKGGVITPQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAAEKAIEDGVPALRAARNMNDLEAAMVVV
DRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRLNTWLSDQPLSLKLSNGSLWQPKNYDRQF
RGQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIPKSVINPVPAMLLGAIDLTPVEVAQEYQTIASGGNRAPLSA
VRSVIAEDGTVLYQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGKYNLAAKTGTTNDLRDSWFAGIDGKEVT
IAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSAGNFVCGEGSGMRVIPVWTENPQALCQGS
TPTQDPTKPNDDGVADWIKEMFGQ

Sequences:

>Translated_824_residues
MSGDDREPIGRKGKKAQPKAAPKRPLRRRRDEDEYEEDYDEEQNDYDDEEEPMPRKVASRPPRKKRRWLGLLIKLFLIGA
VALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMSYSKKEMIALLEGMQYRQVSRMTRPGEFTVQNDSIDILRR
PFDFPDGKEGQIRARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNGEQRLFVPRAGFPDLLVDTLLATEDRHF
YEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLNEAYMALLMDYRYSKDRILELYLNEVYLG
QSGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGASLYNPWRNPKLALERRNLVLRLLQNQGIIDAELYTMLSAR
PLGVQPKGGVITPQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAAEKAIEDGVPALRAARNMNDLEAAMVVV
DRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRLNTWLSDQPLSLKLSNGSLWQPKNYDRQF
RGQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIPKSVINPVPAMLLGAIDLTPVEVAQEYQTIASGGNRAPLSA
VRSVIAEDGTVLYQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGKYNLAAKTGTTNDLRDSWFAGIDGKEVT
IAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSAGNFVCGEGSGMRVIPVWTENPQALCQGS
TPTQDPTKPNDDGVADWIKEMFGQ
>Mature_823_residues
SGDDREPIGRKGKKAQPKAAPKRPLRRRRDEDEYEEDYDEEQNDYDDEEEPMPRKVASRPPRKKRRWLGLLIKLFLIGAV
ALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMSYSKKEMIALLEGMQYRQVSRMTRPGEFTVQNDSIDILRRP
FDFPDGKEGQIRARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNGEQRLFVPRAGFPDLLVDTLLATEDRHFY
EHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLNEAYMALLMDYRYSKDRILELYLNEVYLGQ
SGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGASLYNPWRNPKLALERRNLVLRLLQNQGIIDAELYTMLSARP
LGVQPKGGVITPQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAAEKAIEDGVPALRAARNMNDLEAAMVVVD
RFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRLNTWLSDQPLSLKLSNGSLWQPKNYDRQFR
GQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIPKSVINPVPAMLLGAIDLTPVEVAQEYQTIASGGNRAPLSAV
RSVIAEDGTVLYQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGKYNLAAKTGTTNDLRDSWFAGIDGKEVTI
AWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSAGNFVCGEGSGMRVIPVWTENPQALCQGST
PTQDPTKPNDDGVADWIKEMFGQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=847, Percent_Identity=68.8311688311688, Blast_Score=1130, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=283, Percent_Identity=34.6289752650177, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1788867, Length=512, Percent_Identity=28.515625, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI1789601, Length=127, Percent_Identity=36.2204724409449, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 91817; Mature: 91686

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDDREPIGRKGKKAQPKAAPKRPLRRRRDEDEYEEDYDEEQNDYDDEEEPMPRKVASR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHCC
PPRKKRRWLGLLIKLFLIGAVALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCEEEECCCCC
SYSKKEMIALLEGMQYRQVSRMTRPGEFTVQNDSIDILRRPFDFPDGKEGQIRARLLFKN
CCHHHHHHHHHHCHHHHHHHHCCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECC
DRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNGEQRLFVPRAGFPDLLVDTLLATEDRHF
CCHHHHHCCCCCCCCCEEEECHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCH
YEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLNEAYMALLM
HHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DYRYSKDRILELYLNEVYLGQSGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGA
HHCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHCCCCCHHHHCCCHHHHHHHHHCCC
SLYNPWRNPKLALERRNLVLRLLQNQGIIDAELYTMLSARPLGVQPKGGVITPQPAFMQM
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAAEKAIEDGVPALRAARNMNDLEAAMVVV
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
DRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRLNTWLSDQP
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCC
LSLKLSNGSLWQPKNYDRQFRGQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIP
CEEEECCCCCCCCCCCCHHHCCCEEEHHHHHHHCCCCEEECCHHCCHHHHHHHHHHHCCC
KSVINPVPAMLLGAIDLTPVEVAQEYQTIASGGNRAPLSAVRSVIAEDGTVLYQSFPQAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHH
RMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGKYNLAAKTGTTNDLRDSWFAGIDGKEVT
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCCEEE
IAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSAGNFVCGEG
EEEEEECCCCCCEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEECCC
SGMRVIPVWTENPQALCQGSTPTQDPTKPNDDGVADWIKEMFGQ
CCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SGDDREPIGRKGKKAQPKAAPKRPLRRRRDEDEYEEDYDEEQNDYDDEEEPMPRKVASR
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHCC
PPRKKRRWLGLLIKLFLIGAVALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCEEEECCCCC
SYSKKEMIALLEGMQYRQVSRMTRPGEFTVQNDSIDILRRPFDFPDGKEGQIRARLLFKN
CCHHHHHHHHHHCHHHHHHHHCCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECC
DRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNGEQRLFVPRAGFPDLLVDTLLATEDRHF
CCHHHHHCCCCCCCCCEEEECHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCH
YEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLNEAYMALLM
HHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DYRYSKDRILELYLNEVYLGQSGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGA
HHCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHCCCCCHHHHCCCHHHHHHHHHCCC
SLYNPWRNPKLALERRNLVLRLLQNQGIIDAELYTMLSARPLGVQPKGGVITPQPAFMQM
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAAEKAIEDGVPALRAARNMNDLEAAMVVV
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
DRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRLNTWLSDQP
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCC
LSLKLSNGSLWQPKNYDRQFRGQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIP
CEEEECCCCCCCCCCCCHHHCCCEEEHHHHHHHCCCCEEECCHHCCHHHHHHHHHHHCCC
KSVINPVPAMLLGAIDLTPVEVAQEYQTIASGGNRAPLSAVRSVIAEDGTVLYQSFPQAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHH
RMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGKYNLAAKTGTTNDLRDSWFAGIDGKEVT
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCCEEE
IAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSAGNFVCGEG
EEEEEECCCCCCEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEECCC
SGMRVIPVWTENPQALCQGSTPTQDPTKPNDDGVADWIKEMFGQ
CCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3882429; 8202364; 9278503; 3920658; 8645198; 9244263; 3330753; 1885547; 10037771; 10564478; 9841666 [H]