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The map label for this gene is btuF [H]

Identifier: 218930402

GI number: 218930402

Start: 3778511

End: 3779353

Strand: Direct

Name: btuF [H]

Synonym: YPO3385

Alternate gene names: 218930402

Gene position: 3778511-3779353 (Clockwise)

Preceding gene: 218930401

Following gene: 218930403

Centisome position: 81.19

GC content: 53.62

Gene sequence:

>843_bases
ATGATGCCACTGGGTCTTTTTCCTCTGCCACGCGCAGCAGCAGTGTTGCTAATTAGCCTGCTAACACTCCCAGCACAGGC
TGCCGAACGGGTTATCAGTTTATCGCCCAGTACCACGGAACTGGCTTATGCTGCAGGTTTGGGCGATAAGTTGGTCGCGG
TCAGTGCGTACTCTGATTACCCGGAATCGGCTAAAAAGTTAGAACATGTGGCGTCGTGGCAAGGCATTAACGTTGAGCGT
ATTCTGGCACTAAAACCCGATCTGATCCTCGCCTGGCGTGGAGGTAATCCACAACGGCCGCTGGATCAATTAGCGGCGTT
GGGTATTCCGATTTTCTACTCTGACCCCACGCATATCGATCAGATTGCCAGCGATCTGGATAAGCTAGCGCAATACAGCC
CGCACCCCGAGCAGGCACATCAGGCCGCTGAACAATTCCGTCAACACGTCAACACACTGCGTGATCGCTATGCCCGCAGT
CAGCCCAAACGTACCTTTTTGCAATTCGGTACTCAGCCACTGTTTACCAGCTCCGGGCACACCTTACAGAGTGAAGTCGT
TTCACTCTGTGGTGGAGAAAATATTTTTGCCGACAGCCGGGTTCCCTGGCCACAAGTCAGTCGTGAGCAAGTGATGACAC
GTAAGCCACAGGTCATCGTGGTAAGCGGTACGCAGTCGCAAGTCGATAACGTCTCCGCCTTTTGGTTGCCTCAGCTTGTG
GTACCTGTTATCGCCCTGAATGAAGACTGGTTTAATCGCGCCAGCCCACGTATCCTGCTGGCCGCTCAACAGCTGTGCCA
ACAAATGGCCAGCATTCCAACCCCTGTCGCGGAGTCACATTAA

Upstream 100 bases:

>100_bases
AAGCATCTCACCTAAGCTTTGAAGAGTTCTTGGTGGTGGCGGCGAAACAATCGACCCTAATGATCAAAGCCATGTTAACC
ACCTTGGCGCAACGCGGTTA

Downstream 100 bases:

>100_bases
TGCTGGTTTATTGGCTGGATATCTTAGGTACTGCGGTATTTGCTATCTCTGGTGTATTACTGGCAGGAAAACTACGTATG
GATCCATTTGGCGTACTGGT

Product: vitamin B12-transporter protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MMPLGLFPLPRAAAVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDYPESAKKLEHVASWQGINVER
ILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHIDQIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARS
QPKRTFLQFGTQPLFTSSGHTLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH

Sequences:

>Translated_280_residues
MMPLGLFPLPRAAAVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDYPESAKKLEHVASWQGINVER
ILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHIDQIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARS
QPKRTFLQFGTQPLFTSSGHTLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH
>Mature_280_residues
MMPLGLFPLPRAAAVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDYPESAKKLEHVASWQGINVER
ILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHIDQIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARS
QPKRTFLQFGTQPLFTSSGHTLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=260, Percent_Identity=54.6153846153846, Blast_Score=291, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30814; Mature: 30814

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLGLFPLPRAAAVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDY
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCC
PESAKKLEHVASWQGINVERILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHID
HHHHHHHHHHHHCCCCCHHHEEEECCCEEEEECCCCCCCHHHHHHHHCCCEEECCCHHHH
QIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARSQPKRTFLQFGTQPLFTSSGH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
TLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
HHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHH
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMPLGLFPLPRAAAVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDY
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCC
PESAKKLEHVASWQGINVERILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHID
HHHHHHHHHHHHCCCCCHHHEEEECCCEEEEECCCCCCCHHHHHHHHCCCEEECCCHHHH
QIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARSQPKRTFLQFGTQPLFTSSGH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
TLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
HHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHH
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA