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The map label for this gene is alaS [H]

Identifier: 218930326

GI number: 218930326

Start: 3683714

End: 3686341

Strand: Reverse

Name: alaS [H]

Synonym: YPO3305

Alternate gene names: 218930326

Gene position: 3686341-3683714 (Counterclockwise)

Preceding gene: 218930327

Following gene: 218930325

Centisome position: 79.21

GC content: 50.0

Gene sequence:

>2628_bases
ATGAGCAAGAGCACCGCTGAGATCCGTCAAGCGTTTCTCGATTTCTTCCATAGCAAGGGACACCAGGTTGTATCAAGCAG
CACTTTGGTCCCTAATAATGACCCTACGCTGTTGTTTACCAATGCCGGGATGAACCAGTTTAAGGACGTTTTCTTAGGTT
TGGATAAACGGGCCTATTCCCGTGCGACGACCTCTCAGCGCTGCGTGCGAGCGGGTGGTAAGCATAACGACCTGGAAAAC
GTAGGTTATACCGCGCGTCATCATACTTTCTTCGAAATGTTGGGTAACTTCAGCTTCGGCGATTACTTCAAGCATGATGC
TATCAACTTCGCTTGGGAGTTACTGACCAGTGAACAGTGGTTTAATTTACCAAAAGAAAAACTTTGGGTAACGGTGTACG
AGACCGATGACGAGGCCTATAACATCTGGGCTAACGAAGTCGGCGTTCCTCATGAGCGTATCATCCGTATCGGTGATAAC
AAAGGGGGGGCCTTTGCCTCAGATAACTTCTGGCAAATGGGTGATACCGGCCCTTGTGGCCCATGTTCAGAGATTTTCTT
TGACCATGGTGATCATATCTGGGGTGGTCCTCCAGGGTCTGCAGAAGAAGATGGCGATCGCTATATCGAGATCTGGAATA
TCGTCTTTATGCAGTTTAACCGTCAGTCTGACGGTACGATGCTGCCATTGCCGAAGCCATCGGTTGATACCGGCATGGGG
CTGGAACGTATTGCTGCGGTATTACAGCATGTTAACTCCAACTATGAGATTGACCTGTTCCGTGATTTGATTGCCGCAGT
CGCAGATGTCACGGGGGCGACGGATTTATCCAGCAAGTCGTTGCGGGTTATTGCTGATCACATTCGTTCTTGTGCTTTCT
TGATTTCAGATGGTGTGATCCCATCGAATGAAAACCGCGGCTATGTCTTACGCCGTATTATTCGCCGTGCAATTCGCCAC
GGGAATATGTTGGGTGCGAAAGAGACTTTCTTCTACAAACTGGTGGCTCCCTTGATCGCGGTTATGGGGCCTGCTGCTGC
TGAGTTAAAACAACAGCAAGCGATGGTAGAGCAAGTATTAAAGACGGAAGAAGAGCAGTTTGCCAGGACGTTAGAGCGCG
GTTTGGCGCTGCTTGATGACGAGCTCAGCAAGCTTACCGGCGATACCCTTGATGGGGAAACGGCCTTCCGCTTGTACGAT
ACCTATGGCTTCCCAGTTGATCTAACGGCAGATGTTTGTCGCGAACGTAACCTGAAAGTCGATGAAGCTGGCTTTGAGCA
AGCGATGGAAGCTCAACGTCGCCGTGCCCGTGAATCCAGTGGTTTTGGCGCAGATTACAACAGCTTGATCCGTGTTGATA
GCGCAAGCCAATTTTCTGGTTATGACCATGTCCAACAACACGCGACGGTTACTGCTCTATTCCGTAATGGCGAGGCCGTT
GATGAGATTCACGCAGGTGAAGAAGCCGTTGTCGTACTGAATCGGACACCATTCTACGGTGAATCGGGTGGTCAGGTCGG
CGATAAGGGGGAACTGAAAAATGCGACAGCAACCTTTAGCGTTACTGATACTCAGAAATATGGTCAGGCTATCGGGCACG
TAGGAATATTGACTACCGGCACGTTAAGAGTTAACCACAGCGTCGAAGCATTGGTTGATGTTGTGCGTCGTAACCGTATC
CGTCTGAATCACTCCGCGACCCACTTACTGCATGCTGCGTTACGTAACGTCTTGGGCGAGCATGTGGCCCAGAAGGGGTC
ACTGGTTAATGACAAATATCTACGCTTTGATTTCTCTCATTTCGAAGCAATGAAGCCGGAACAAATCCGGTTGGTAGAAG
ATCTGGTCAATGAACAAATCCGCCGTAACATGCCGGTTCAGACCGAAGTGATGGAACTGGATGCGGCGAAAGAAAAAGGT
GCAATGGCACTGTTCGGCGAGAAGTATGACGACCAAGTACGTGTTCTGACCATGGGTGACTTCTCAACAGAGTTGTGTGG
GGGGACTCATGCTAGCCGCACGGGGGATATAGGGTTATTCCGCATCCTGACCGAATCTGGCACGGCTGCGGGTATTCGTC
GTATTGAAGCCGTCACTGGCGAGGGCGCGATTGCTTTATTGCATCAGCAAAGCGACCTGTTACAGGACGTCGCTCATCTG
GTGAAAGGTGATATTCATAATCTGGCAGACAAAGTCCGGGCGGTATTGGATCGCAGTAAAATGCTTGAGCGTGAGTTGCA
ACAGTTGAAAGATCAGCAGGCAGCTCAGGAAAGTGCCTCGTTGTCTTCAAGCGCGAAATTGATCAATGGTGTGAAGCTTT
TGGTCAGTCAGTTGGATAACGTTGAACCAAAAATGCTACGGACAATGGTCGATGACCTTAAAAATCAACTGGGTTCTGCC
ATTATTGTATTGGCCACCACAGCTGATGATAAGGTAAGTTTGATTGTTGGGGTGACCAAGGACCTGACAGGTAAAGTAAA
AGCGGGTGAATTAATCGCGGACATTGCTCAGCAGGTGGGCGGTAAAGGCGGTGGTCGTCCTGATATGGCTCAGGCGGGGG
GTACTGATGTGCAGGCTTTGCCAGCGGCATTAGCCAGCGTAGAAGCATGGGTAGCATCCCGGATGTAA

Upstream 100 bases:

>100_bases
CTTATTCCAACTTTTGTTCGTGAGCTTATCCATTGCGTTGTCGCTAATCTGTACACGATTTGCTCACGGGCCATCCTTTT
AGCTTGATTTCAGGACAATT

Downstream 100 bases:

>100_bases
GTAATCATCTATTAAATCAACACGCCATATCTCGGTACTAGGTATGGCGTTTTTTAATTTTCCTGTCATAGTTCAATCAC
AAAGTTAAACGCAAAGTTGT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MSKSTAEIRQAFLDFFHSKGHQVVSSSTLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQWFNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDN
KGGAFASDNFWQMGDTGPCGPCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVIPSNENRGYVLRRIIRRAIRH
GNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVLKTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYD
TYGFPVDLTADVCRERNLKVDEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTGTLRVNHSVEALVDVVRRNRI
RLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKG
AMALFGEKYDDQVRVLTMGDFSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDNVEPKMLRTMVDDLKNQLGSA
IIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVGGKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM

Sequences:

>Translated_875_residues
MSKSTAEIRQAFLDFFHSKGHQVVSSSTLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQWFNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDN
KGGAFASDNFWQMGDTGPCGPCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVIPSNENRGYVLRRIIRRAIRH
GNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVLKTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYD
TYGFPVDLTADVCRERNLKVDEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTGTLRVNHSVEALVDVVRRNRI
RLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKG
AMALFGEKYDDQVRVLTMGDFSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDNVEPKMLRTMVDDLKNQLGSA
IIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVGGKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM
>Mature_874_residues
SKSTAEIRQAFLDFFHSKGHQVVSSSTLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLENV
GYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQWFNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDNK
GGAFASDNFWQMGDTGPCGPCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMGL
ERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVIPSNENRGYVLRRIIRRAIRHG
NMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVLKTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYDT
YGFPVDLTADVCRERNLKVDEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAVD
EIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTGTLRVNHSVEALVDVVRRNRIR
LNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKGA
MALFGEKYDDQVRVLTMGDFSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHLV
KGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDNVEPKMLRTMVDDLKNQLGSAI
IVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVGGKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=988, Percent_Identity=37.6518218623482, Blast_Score=549, Evalue=1e-156,
Organism=Homo sapiens, GI38569417, Length=971, Percent_Identity=33.8825952626159, Blast_Score=454, Evalue=1e-127,
Organism=Escherichia coli, GI1789048, Length=875, Percent_Identity=82.4, Blast_Score=1508, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=977, Percent_Identity=35.6192425793245, Blast_Score=525, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17536681, Length=756, Percent_Identity=32.6719576719577, Blast_Score=370, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324911, Length=809, Percent_Identity=41.7799752781211, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24582809, Length=979, Percent_Identity=36.6700715015322, Blast_Score=552, Evalue=1e-157,
Organism=Drosophila melanogaster, GI45552267, Length=979, Percent_Identity=36.6700715015322, Blast_Score=552, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24658214, Length=824, Percent_Identity=30.3398058252427, Blast_Score=341, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96332; Mature: 96201

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSTAEIRQAFLDFFHSKGHQVVSSSTLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYS
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHH
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHH
FNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDNKGGAFASDNFWQMGDTGPCG
HCCCCHHEEEEEEECCCCCEEEEHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
CHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEEEEHHEECCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAIRHGNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYDTYGFPVDLTADVCRERNLKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
CHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCHHCCCHHHHHHHHHHEEHCCCCCHH
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTG
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEEC
TLRVNHSVEALVDVVRRNRIRLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSH
EEEECCCHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHH
FEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKGAMALFGEKYDDQVRVLTMGD
HHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECC
FSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
CCHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDN
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVG
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSTAEIRQAFLDFFHSKGHQVVSSSTLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYS
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHH
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHH
FNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDNKGGAFASDNFWQMGDTGPCG
HCCCCHHEEEEEEECCCCCEEEEHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
CHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEEEEHHEECCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAIRHGNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYDTYGFPVDLTADVCRERNLKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
CHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCHHCCCHHHHHHHHHHEEHCCCCCHH
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTG
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEEC
TLRVNHSVEALVDVVRRNRIRLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSH
EEEECCCHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHH
FEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKGAMALFGEKYDDQVRVLTMGD
HHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECC
FSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
CCHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDN
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVG
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA