Click here to switch to the map view.

The map label for this gene is clpB

Identifier: 218930300

GI number: 218930300

Start: 3654692

End: 3657265

Strand: Reverse

Name: clpB

Synonym: YPO3275

Alternate gene names: 218930300

Gene position: 3657265-3654692 (Counterclockwise)

Preceding gene: 218930301

Following gene: 218930294

Centisome position: 78.59

GC content: 47.9

Gene sequence:

>2574_bases
ATGCGTCTGGATCGTCTTACCAGTAAATTCCAGCTTGCCCTCGCCGATGCCCAGTCTTTAGCTCTTGGGCGCGACAACCA
GTTTATTGAACCGTTACATTTGATGAGCGCACTGCTTAATCAGGATGGGGGGACGGTTCGCCCTTTATTGACCTCGGCAG
GGATTAATGTTGCACGTTTACGTAGCGACATTGAGCAAGCTTTAGGTCGTCTTCCTCAAGTTGAAGGCACCGGCGGTGAT
GTTCAACCATCAAACGAGCTGGTTCGTGTATTAAATCTTTGCGACAAATTAGCGCAGAAACGTTCGGATAAGTTTATCTC
TTCCGAGCTGTTCGTCCTGGCCGTACTTGAAGATCGTGGCACGTTAACTGACATGTTAAAGGCTGCAGGTGCAACCGTCG
CTATAATAGATAAGGCAATTGAAACCATGCGTGGTGGTGACAAAGTCGATGATCAGGGTGCTGAAGATCAGCGCCAGGCG
TTGAAAAAATTCACTATCGACCTGACTGAGCGTGCCGAGCAAGGCAAACTCGATCCGGTTATTGGCCGTGATGAAGAAAT
TCGCCGTACTATTCAGGTATTACAACGTCGAACCAAAAATAACCCAGTGTTGATTGGTGAGCCTGGGGTGGGTAAAACCG
CTATTGTGGAAGGTTTGGCGCAACGCATTGTGAACGGCGAAGTGCCAGAAGGCTTGAAGCATAAGCGTGTACTCTCATTA
GATATGGGGTCGCTGATTGCCGGTGCCAAATATCGTGGTGAATTTGAAGAGCGTTTGAAAGGCGTACTGAACGACTTATC
AAAACAAGAAGGCAGTGTGATCTTGTTTATTGATGAGTTGCATACCATGGTCGGTGCCGGTAAAGGCGATGGGGCGATGG
ATGCCGGTAACATGCTGAAACCTGCATTAGCGCGAGGTGAGTTACATTGTGTGGGGGCCACTACCCTCGATGAATACCGT
CAATATATAGAGAAAGATGCTGCGCTCGAGCGGCGTTTCCAAAAAGTGTATGTTGCGGAACCAACGGTAGAAGACACTAT
CGCTATTTTACGTGGGCTGAAAGAGCGCTACGAACTGCACCATCACGTGCAGATCACTGACCCAGCTATTGTGGCAGCCG
CAACATTGTCACACCGGTATATTTCTGATCGTCAGTTACCGGATAAAGCTATCGACCTCATTGATGAGGCAGGTTCCAGC
ATCCGTATGCAAATGGATTCAAAACCCGAATCATTGGATCGTCTAGAGCGCCGGATTATTCAGCTTAAACTGGAACAGCA
GGCCCTAAAAAAAGAGTCAGATGACGCCAGTAAAAAACGGTTGGAAATGCTCAATACTGAGTTAGAGCAAAAAGAGCGTG
AATATTCTGAACTGGAAGAAGAGTGGAAAGCAGAAAAAGCTTCACTCACCGGGACTCAGAATATCAAGACGGAGTTGGAA
CAAGCTAAAATCACCTTAGAACAAGCTCGTCGAGTAGGTGATCTGGCTCGGATGTCAGAACTTCAGTACGGTACAATCCC
TGAGCTGGAAAAACAGCTGGCGGCGGCAACCGCGCTAGAAGGGAAAACCATGAAGTTGCTGCGTAACCGTGTCACAGAAA
TCGAAGTTGCTGAAGTCCTGGCTCGCTGGACCGGTATCCCGGTATCTCGCATGTTGAAAAGTGAGCGGGATAAGCTGCTA
CGTATGGAACAGGACTTGCATCAACGAGTCATTGGGCAAGATGAAGCTGTTGAAGCCGTCTCCAACGCTATCCGTCGGAG
TCGCGCAGGGTTGTCTGATCCAAACCGACCAATTGGTTCGTTCTTATTCTTAGGGCCAACGGGGGTTGGTAAAACTGAAT
TGTGTAAAGCGTTGGCAACGTTCTTGTTCGACAGTGACGATGCCATGGTGCGAATCGACATGTCCGAGTTTATGGAGAAA
CATTCCGTATCGCGGTTGGTCGGTGCACCTCCTGGCTATGTTGGGTATGAAGAAGGTGGCTATCTGACGGAAGCGGTTCG
TCGTCGTCCTTATTCTGTGATTTTGCTGGATGAAGTTGAAAAAGCACATCCAGATGTTTTCAACATATTGTTACAGGTAT
TGGATGATGGTCGTTTGACCGACGGGCAGGGGAGAACTGTCGACTTTCGTAACACGGTAGTCATTATGACCTCTAACCTT
GGTTCAGATTTGATTCAGGAACATTTCGGCCAAATGAATTACAGCGAAATGAAGACAGTGGTTATGGATGTTGTTAGTCA
CCACTTCCGTCCTGAATTTATTAACCGTATAGATGAGGTTGTTGTCTTCCACCCACTTGGTCGGGCGCACTTGGCTTCTA
TTGCCAACATTCAACTGGAGCGTTTGTATAAACGTCTGGAAGAACGTGGCTATGAAGTGAAGATTACTCAGCCTGCATTG
GAATTCTTGGGTGAAGCTGGCTTCGACCCTGTTTATGGGGCGCGGCCATTAAAACGTGCAATTCAGCAGGAAATTGAGAA
CCCGCTGGCACAGCAGATACTTTCTGGCAAACTAATACCGGGAATCCCCGTGACTTTAGATGTTGAAAATGGGCATATTA
TTGCTAAGCAGTAA

Upstream 100 bases:

>100_bases
AGAACGCATTATTTTAATATAGTGTCAAAATAACCTTGAAAAATTGAGGGATGACCTCATTTAATCTCCAGTAGCAATTT
TGACCAATATTGGAGGTGTT

Downstream 100 bases:

>100_bases
TAGCTGATCCTCTAAGGGGCGAGAAATCGCCCCTTTTTTGTTGTTTTTTTCTTCATATATTGCATTTATGATGCATTAGT
GAGCGGTTGAAAAGTTTTTT

Product: protein disaggregation chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQVEGTGGD
VQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQA
LKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSS
IRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLKSERDKLL
RMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEVKITQPAL
EFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ

Sequences:

>Translated_857_residues
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQVEGTGGD
VQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQA
LKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSS
IRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLKSERDKLL
RMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEVKITQPAL
EFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ
>Mature_857_residues
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQVEGTGGD
VQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQA
LKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSS
IRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLKSERDKLL
RMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEVKITQPAL
EFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=320, Percent_Identity=37.1875, Blast_Score=218, Evalue=3e-56,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=86.5810968494749, Blast_Score=1525, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=419, Percent_Identity=39.8568019093079, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6320464, Length=710, Percent_Identity=53.0985915492958, Blast_Score=762, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=838, Percent_Identity=43.0787589498807, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_YERPE (Q74X11)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AI0397
- RefSeq:   NP_668245.1
- RefSeq:   NP_992046.1
- RefSeq:   YP_002348175.1
- ProteinModelPortal:   Q74X11
- GeneID:   1145861
- GeneID:   1176093
- GeneID:   2766388
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3275
- KEGG:   ypk:y0914
- KEGG:   ypm:YP_0656
- HOGENOM:   HBG413133
- OMA:   YITERRL
- ProtClustDB:   PRK10865
- BioCyc:   YPES187410:Y0914-MONOMER
- BioCyc:   YPES214092:YPO3275-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- PRINTS:   PR00300
- SMART:   SM00382
- TIGRFAMs:   TIGR03346

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 95646; Mature: 95646

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHH
RSDIEQALGRLPQVEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
TLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQALKKFTIDLTERAEQGKLDPV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLK
CHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSSIRMQMDSKPESLDRLERRII
HCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
QLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ARWTGIPVSRMLKSERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLE
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RLYKRLEERGYEVKITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIP
HHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GIPVTLDVENGHIIAKQ
CCCEEEEECCCEEEECC
>Mature Secondary Structure
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHH
RSDIEQALGRLPQVEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
TLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQALKKFTIDLTERAEQGKLDPV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLK
CHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSSIRMQMDSKPESLDRLERRII
HCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
QLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ARWTGIPVSRMLKSERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLE
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RLYKRLEERGYEVKITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIP
HHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GIPVTLDVENGHIIAKQ
CCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430