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The map label for this gene is nanK

Identifier: 218930067

GI number: 218930067

Start: 3373928

End: 3374800

Strand: Reverse

Name: nanK

Synonym: YPO3020

Alternate gene names: 218930067

Gene position: 3374800-3373928 (Counterclockwise)

Preceding gene: 218930070

Following gene: 218930066

Centisome position: 72.52

GC content: 56.59

Gene sequence:

>873_bases
ATGGGAAAAGGGTTAGCACTGGATATCGGTGGAACAAAAATTGCCGCCGCGGTGGTCACTGAGAGTGGCATGTTGATTGG
CCGCCAGCAAATAGCAACACCACGAGGCGGTGCCGGACAACTGGCTGCCGCACTGGAAACACTGATAGCGCCTTACCGCC
ATCAGGTTGATTTTATTGCTGTTGCGTCAACGGGCATTATCAGCGGGGGCCGTCTGACGGCGCTCAATCCGGCCAATTTG
GGCGGTCTGGCTGACTTCCCATTGTACGACTGTATTCGCTCTATCAGTGATCTGCCTTGTGTCCTGCTGAACGACGGTCA
GGCTGCGGCATGGGCTGAATACCAGGCGCTGGGTGATAAAAATGACAATATGATGTTTGTGACCGTTTCCACTGGTGTGG
GCGGCGGCATCATTTTGAATAAAAAACTGTTGGTCGGGCAACGTGGGCTGGCAGGGCATATCGGCCATACGCTGTCTGAT
CCTCATGGTGTTTTGTGTGGTTGCGGTCGCCGGGGATGTGTCGAGAGCGTGGCATCAGGCACGGCTATTGGGGCAGAAAC
CTTGGGGTGGAAGCAACCGGTATCGGCTGCCACGGTGTTTGACATGGCGCAGCAAGGGGATGCGCAGGCGGGAAAAGTCA
TTAACCGTTCTGCCGCAGCCATTGCTCAGATGTTGGCCGACATGAAGATGGCGCTGGATCTGGAGGTGGTTATTCTGGGG
GGCAGTGTCGGGTTGGCGGTTGGCTACCTGGAGAGGGTGGTTGCCGCCCAGAAGACATTACCGGGTATTTACCGCGTCCC
CGTACAAGAGGCACATCACCGTCAGGATAGTGGATTACTCGGGGCGGCACTGTGGGCCAGAACGTCGTTGTGA

Upstream 100 bases:

>100_bases
ACTCCAAACTAAAAATAACAATGGAGTTTGCGCTAAAAGATTGGCATAGTTATCCATTCAGCGTGAGTAGACTCGTTAAG
ATACAAGAGGGTTACGCCAG

Downstream 100 bases:

>100_bases
TCACGATCAGTGATATACCGTTATTGAGTCACTTACCGTTATCAGTAAATATACCGTCATCAGTCAATATCGAGGCATAA
CATGATCAGTGGATCCTTGG

Product: N-acetylmannosamine kinase

Products: NA

Alternate protein names: ManNAc kinase; N-acetyl-D-mannosamine kinase

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MGKGLALDIGGTKIAAAVVTESGMLIGRQQIATPRGGAGQLAAALETLIAPYRHQVDFIAVASTGIISGGRLTALNPANL
GGLADFPLYDCIRSISDLPCVLLNDGQAAAWAEYQALGDKNDNMMFVTVSTGVGGGIILNKKLLVGQRGLAGHIGHTLSD
PHGVLCGCGRRGCVESVASGTAIGAETLGWKQPVSAATVFDMAQQGDAQAGKVINRSAAAIAQMLADMKMALDLEVVILG
GSVGLAVGYLERVVAAQKTLPGIYRVPVQEAHHRQDSGLLGAALWARTSL

Sequences:

>Translated_290_residues
MGKGLALDIGGTKIAAAVVTESGMLIGRQQIATPRGGAGQLAAALETLIAPYRHQVDFIAVASTGIISGGRLTALNPANL
GGLADFPLYDCIRSISDLPCVLLNDGQAAAWAEYQALGDKNDNMMFVTVSTGVGGGIILNKKLLVGQRGLAGHIGHTLSD
PHGVLCGCGRRGCVESVASGTAIGAETLGWKQPVSAATVFDMAQQGDAQAGKVINRSAAAIAQMLADMKMALDLEVVILG
GSVGLAVGYLERVVAAQKTLPGIYRVPVQEAHHRQDSGLLGAALWARTSL
>Mature_289_residues
GKGLALDIGGTKIAAAVVTESGMLIGRQQIATPRGGAGQLAAALETLIAPYRHQVDFIAVASTGIISGGRLTALNPANLG
GLADFPLYDCIRSISDLPCVLLNDGQAAAWAEYQALGDKNDNMMFVTVSTGVGGGIILNKKLLVGQRGLAGHIGHTLSDP
HGVLCGCGRRGCVESVASGTAIGAETLGWKQPVSAATVFDMAQQGDAQAGKVINRSAAAIAQMLADMKMALDLEVVILGG
SVGLAVGYLERVVAAQKTLPGIYRVPVQEAHHRQDSGLLGAALWARTSL

Specific function: Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NanK subfamily

Homologues:

Organism=Escherichia coli, GI87082230, Length=273, Percent_Identity=49.4505494505495, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=29.1390728476821, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081733, Length=318, Percent_Identity=24.8427672955975, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87082127, Length=175, Percent_Identity=29.1428571428571, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1786891, Length=167, Percent_Identity=26.3473053892216, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787878, Length=254, Percent_Identity=24.4094488188976, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NANK_YERP3 (A7FG96)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400274.1
- ProteinModelPortal:   A7FG96
- SMR:   A7FG96
- STRING:   A7FG96
- GeneID:   5387903
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1295
- eggNOG:   COG1940
- HOGENOM:   HBG730260
- OMA:   NIMEDIT
- ProtClustDB:   PRK05082
- BioCyc:   YPSE349747:YPSIP31758_1295-MONOMER
- HAMAP:   MF_01234
- InterPro:   IPR000600

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.60

Molecular weight: Translated: 29746; Mature: 29615

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKGLALDIGGTKIAAAVVTESGMLIGRQQIATPRGGAGQLAAALETLIAPYRHQVDFIA
CCCCEEEEECCCEEEEEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEE
VASTGIISGGRLTALNPANLGGLADFPLYDCIRSISDLPCVLLNDGQAAAWAEYQALGDK
EECCCEECCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCC
NDNMMFVTVSTGVGGGIILNKKLLVGQRGLAGHIGHTLSDPHGVLCGCGRRGCVESVASG
CCCEEEEEEECCCCCCEEEECEEEECCCCCCHHCCCCCCCCCCEEECCCCHHHHHHHHCC
TAIGAETLGWKQPVSAATVFDMAQQGDAQAGKVINRSAAAIAQMLADMKMALDLEVVILG
CCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
GSVGLAVGYLERVVAAQKTLPGIYRVPVQEAHHRQDSGLLGAALWARTSL
CCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
GKGLALDIGGTKIAAAVVTESGMLIGRQQIATPRGGAGQLAAALETLIAPYRHQVDFIA
CCCEEEEECCCEEEEEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEE
VASTGIISGGRLTALNPANLGGLADFPLYDCIRSISDLPCVLLNDGQAAAWAEYQALGDK
EECCCEECCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCC
NDNMMFVTVSTGVGGGIILNKKLLVGQRGLAGHIGHTLSDPHGVLCGCGRRGCVESVASG
CCCEEEEEEECCCCCCEEEECEEEECCCCCCHHCCCCCCCCCCEEECCCCHHHHHHHHCC
TAIGAETLGWKQPVSAATVFDMAQQGDAQAGKVINRSAAAIAQMLADMKMALDLEVVILG
CCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
GSVGLAVGYLERVVAAQKTLPGIYRVPVQEAHHRQDSGLLGAALWARTSL
CCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA